Celastrol enhances the viability of random-pattern skin flaps by regulating autophagy through the AMPK-mTOR-TFEB axis.

In reconstructive and plastic surgery, random-pattern skin flaps (RPSF) are often used to correct defects. However, their clinical usefulness is limited due to their susceptibility to necrosis, especially on the distal side of the RPSF. This study validates the protective effect of celastrol (CEL) on flap viability and explores in terms of underlying mechanisms of action. The viability of different groups of RPSF was evaluated by survival zone analysis, laser doppler blood flow, and histological analysis. The effects of CEL on flap angiogenesis, apoptosis, oxidative stress, and autophagy were evaluated by Western blot, immunohistochemistry, and immunofluorescence assays. Finally, its mechanistic aspects were explored by autophagy inhibitor and Adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) inhibitor. On the seventh day after surgery, the survival area size, blood supply, and microvessel count of RPSF were augmented following the administration of CEL. Additionally, CEL stimulated angiogenesis, suppressed apoptosis, and lowered oxidative stress levels immediately after elevated autophagy in ischemic regions; These effects can be reversed using the autophagy inhibitor chloroquine (CQ). Specifically, CQ has been observed to counteract the protective impact of CEL on the RPSF. Moreover, it has also been discovered that CEL triggers the AMPK-mTOR-TFEB axis activation in the area affected by ischemia. In CEL-treated skin flaps, AMPK inhibitors were demonstrated to suppress the AMPK-mTOR-TFEB axis and reduce autophagy levels. This investigation suggests that CEL benefits the survival of RPSF by augmenting angiogenesis and impeding oxidative stress and apoptosis. The results are credited to increased autophagy, made possible by the AMPK-mTOR-TFEB axis activation.

A novel SpaSA based hyper-parameter optimized FCEDN with adaptive CNN classification for skin cancer detection.

Skin cancer is the most prevalent kind of cancer in people. It is estimated that more than 1 million people get skin cancer every year in the world. The effectiveness of the disease's therapy is significantly impacted by early identification of this illness. Preprocessing is the initial detecting stage in enhancing the quality of skin images by removing undesired background noise and objects. This study aims is to compile preprocessing techniques for skin cancer imaging that are currently accessible. Researchers looking into automated skin cancer diagnosis might use this article as an excellent place to start. The fully convolutional encoder-decoder network and Sparrow search algorithm (FCEDN-SpaSA) are proposed in this study for the segmentation of dermoscopic images. The individual wolf method and the ensemble ghosting technique are integrated to generate a neighbour-based search strategy in SpaSA for stressing the correct balance between navigation and exploitation. The classification procedure is accomplished by using an adaptive CNN technique to discriminate between normal skin and malignant skin lesions suggestive of disease. Our method provides classification accuracies comparable to commonly used incremental learning techniques while using less energy, storage space, memory access, and training time (only network updates with new training samples, no network sharing). In a simulation, the segmentation performance of the proposed technique on the ISBI 2017, ISIC 2018, and PH2 datasets reached accuracies of 95.28%, 95.89%, 92.70%, and 98.78%, respectively, on the same dataset and assessed the classification performance. It is accurate 91.67% of the time. The efficiency of the suggested strategy is demonstrated through comparisons with cutting-edge methodologies.

Genome assembly of autotetraploid Actinidia arguta highlights adaptive evolution and enables dissection of important economic traits.

Actinidia arguta, the most widely distributed Actinidia species and the second cultivated species in the genus, can be distinguished from the currently cultivated Actinidia chinensis on the basis of its small and smooth fruit, rapid softening, and excellent cold tolerance. Adaptive evolution of tetraploid Actinidia species and the genetic basis of their important agronomic traits are still unclear. Here, we generated a chromosome-scale genome assembly of an autotetraploid male A. arguta accession. The genome assembly was 2.77 Gb in length with a contig N50 of 9.97 Mb and was anchored onto 116 pseudo-chromosomes. Resequencing and clustering of 101 geographically representative accessions showed that they could be divided into two geographic groups, Southern and Northern, which first diverged 12.9 million years ago. A. arguta underwent two prominent expansions and one demographic bottleneck from the mid-Pleistocene climate transition to the late Pleistocene. Population genomics studies using paleoclimate data enabled us to discern the evolution of the species' adaptation to different historical environments. Three genes (AaCEL1, AaPME1, and AaDOF1) related to flesh softening were identified by multi-omics analysis, and their ability to accelerate flesh softening was verified through transient expression assays. A set of genes that characteristically regulate sexual dimorphism located on the sex chromosome (Chr3) or autosomal chromosomes showed biased expression during stamen or carpel development. This chromosome-level assembly of the autotetraploid A. arguta genome and the genes related to important agronomic traits will facilitate future functional genomics research and improvement of A. arguta.

LINC00662 promotes melanoma progression by competitively binding miR-107 and activating the ß-catenin signaling pathway.

Melanoma is a highly malignant tumor in the body. Long non-coding RNAs (lncRNAs) have been reported to be involved in the development of various tumors. Emerging evidence demonstrates the critical role of lncRNAs in melanoma development. In this study, we aimed to investigate the expression, biological function and regulatory mechanism of LINC00662 in melanomas. First, we found that LINC00662 was up-regulated in melanoma tissues and cell lines. High expression of LINC00662 in melanomas was associated with a poor patient prognosis. Silencing of LINC00662 suppressed the proliferation, migration, and invasion of melanoma cells in vitro and in vivo, while overexpression of LINC00662 promoted melanoma cell proliferation in vitro. Bioinformatics analysis, dual-luciferase assay, and RIP assay confirmed that LINC00662 competitively regulated miR-107. Silencing of LINC00662 upregulated miR-107 expression in a melanoma cell line. Inhibition of miR-107 significantly reversed the inhibitory effect of LINC00662 silencing on cell proliferation and migration. Furthermore, POU3F2 was validated as a downstream target of LINC00662/miR107 and was downregulated when LINC00662 was silenced. Overexpressing POU3F2 attenuated the effect of si-LINC00662 on cellular functions. In addition, the results also showed that the ß-catenin pathway was involved in a si-LINC00662-induced function in melanoma. Overall, our results confirmed that LINC00662 promoted melanoma progression by sponging miR107 and inducing POU3F2, highlighting the mechanism of the LINC00662/miR-107/POU3F2 axis in melanoma cell proliferation and invasion.

Occurrence characteristics and risk assessment of microplastics in agricultural soils in the loess hilly gully area of Yan' an, China.

Large amounts of microplastics accumulated in the soil of agricultural fields with the rapid development of mulch agriculture. The enrichment of microplastics endangered the growth of crops and food security, and it also posed ecological risks. In this study, we investigated microplastics in a typical agriculture area of Yan' an City, in the loess hilly gully area of China. The characteristics of microplastics including their abundances, sizes, and types were measured through laser direct infrared spectrometer. The potential sources of microplastics were analyzed and the risk of soil microplastic pollution was evaluated. It was elaborated that the average abundances of microplastics in soil, water, and fertilizer were 4505 ± 435 n·kg-1, 91 ± 27 n·L-1, and 39,629 ± 10,114 n·kg-1, respectively. Microplastics with particle sizes < 100 µm accounted for >90 %. The smaller the particle size, the higher the content of microplastics. The top three polymers were polyethylene (PE, 37.4 %), polyethylene terephthalate (PET, 15.0 %), and ethylene vinyl acetate (EVA, 8.9 %), respectively. Agricultural mulch, plastic film, domestic waste, surface water irrigation, and organic compost were probably the potential sources of soil microplastics. The ecological risk evaluation showed that overall sampling sites had a minor ecological risk of microplastic pollution based on their abundance, while the polymer type showed a relatively high ecological risk for the investigated agricultural soils. Polyvinylchloride (PVC) and polymethylmethacrylate (PMMA) contribute considerably to the ecological risk, and their inputs to the farmland environment should be strictly limited. There was no significant carcinogenic risk to humans. This study would provide the basic reference for the current situation and risk assessment of farmland soil microplastics pollution in the loess hilly gully area of China.

A Case of Pseudohypoparathyroidism Misdiagnosed as Idiopathic Epilepsy for 5 Years: Clinical Analysis and Follow-up Outcomes.

We report a 15-year-old Chinese girl who presented with intermittent seizure episodes and had been misdiagnosed as having idiopathic epilepsy 5 years previously. Laboratory testing revealed hypocalcemia, hyperphosphatemia, and a high parathyroid hormone (PTH) concentration. She was subsequently shown to have pseudohypoparathyroidism type Ib (PHPIb) based on the results of methylation analysis of the GNAS gene, which showed a loss of methylation of the differentially methylated regions (DMR) of GNAS-AS1, GNAS-XL, and GNAS-A/B; and a gain of methylation of the DMR of the GNAS-NESP55 region. We adjusted the patient's medication by prescribing calcium and calcitriol supplements, and gradually reduced the doses of antiepileptic drugs, until they had been completely discontinued. As a result, the patient did not experience any further seizures or epileptiform symptoms; and had normal plasma calcium, phosphorus, and 25-hydroxyvitamin D concentrations and 24-hour urinary calcium excretion. In addition, her PTH concentration gradually normalized over 12 months, and no urinary stones were found on ultrasonographic examination. In conclusion, the clinical presentation of PHP is complex, and the condition is often misdiagnosed. The diagnosis and follow-up of the present patient have provide valuable insights that should contribute to informed clinical decision-making and the implementation of appropriate treatment strategies.

Characterization and Risk Factors of Folliculitis after Hair Transplantation: A Multicenter Retrospective Study.

BACKGROUND: Postoperative folliculitis is a common complication of hair transplantation (HT) requiring effective preventive interventions. This study characterized postoperative folliculitis and determined risk factors in patients underwent HT. METHODS: We retrospectively reviewed 1317 patients who underwent HT and completed 9-month follow-up between January 2018 and June 2021 at four medical centers. The incidence of postoperative folliculitis and patient demographics were assessed. Logistic regression analysis was used to identify risk factors, and the characteristics of different types of folliculitis were compared. RESULTS: The overall incidence of postoperative folliculitis was 12.11%, and clinical characteristics varied among the different types of folliculitis. Surgery in summer (odds ratio [OR], 1.772, 95% confidence interval [CI]: 1.05-2.992), number of transplant grafts ≥4000 (OR: 4.818, 95% CI: 1.45-16.014), transplant density >45 grafts per/cm 2 (OR: 2.152, 95% CI: 1.376-3.367), and first nursing time >3 days (OR: 1.555, 95% CI: 1.088-2.223) were the main risk factors for postoperative folliculitis. CONCLUSIONS: Postoperative folliculitis after HT presents different characteristics. Surgical factors and postoperative nursing were demonstrated to be related to folliculitis. Therefore, we propose a preventive folliculitis model based on preoperative, intraoperative, and postoperative factors.

Nutrition and melanoma: the contribution of trace elements in onset, progression, and treatment of melanoma.

Melanoma is a highly malignant and drug-resistant disease that imposes a substantial economic burden on the world. There are many studies linking trace elements to diverse types of cancers, including melanoma. This review elucidates the relationship between trace elements exposure and melanoma. It was identified that copper, manganese, selenium, zinc, iron, and many other trace elements were associated with melanoma in humans. In terms of epidemiology, different elements have different correlations with melanoma. These trace elements affect the occurrence and development of melanoma through various mechanisms, such as oxidative stress and the MAPK pathway. The literature on the role of trace elements in the pathogenesis and treatment of melanoma depicts promising prospects for this field.

Eye tracking and eye expression decoding based on transparent, flexible and ultra-persistent electrostatic interface.

Eye tracking provides valuable insight for analyzing visual attention and underlying thinking progress through the observation of eye movements. Here, a transparent, flexible and ultra-persistent electrostatic sensing interface is proposed for realizing active eye tracking (AET) system based on the electrostatic induction effect. Through a triple-layer structure combined with a dielectric bilayer and a rough-surface Ag nanowire (Ag NW) electrode layer, the inherent capacitance and interfacial trapping density of the electrostatic interface has been strongly enhanced, contributing to an unprecedented charge storage capability. The electrostatic charge density of the interface reached 1671.10 µC·m-2 with a charge-keeping rate of 96.91% after 1000 non-contact operation cycles, which can finally realize oculogyric detection with an angular resolution of 5°. Thus, the AET system enables real-time decoding eye movements for customer preference recording and eye-controlled human-computer interaction, supporting its limitless potentiality in commercial purpose, virtual reality, human computer interactions and medical monitoring.

A curtain purification system based on a rabbit fur-based rotating triboelectric nanogenerator for efficient photocatalytic degradation of volatile organic compounds.

Efficient removal of air pollution caused by volatile organic compounds (VOCs) and particulate matter (PM) through distributed energy collected from the environment is an effective strategy to achieve both energy conservation and better air quality. Herein, a curtain purification system based on a rabbit fur-based rotary triboelectric nanogenerator (RR-TENG) and a collaborative photocatalysis technology was designed for indoor air purification. The high electrostatic field from RR-TENG enhances formaldehyde adsorption, while it can also efficiently adsorb PM2.5 simultaneously. More interestingly, the ultrahigh electric field provided by RR-TENG promotes the separation of photogenerated electron-hole pairs of the g-C3N4/TiO2 composite photocatalyst, generating more superoxide radicals (⋅O2-), hydroxyl radicals (⋅OH), and holes (h+) and thereby improving the photocatalytic efficiency. In a simulated reaction chamber of 9 L, the formaldehyde removal rate of the system can reach 79.2% within 90 min and RR-TENG rapidly reduces PM2.5 from 999 µg m-3 to 50 µg m-3 within 60 s. This study proposes a curtain purification system integrating the function of energy collection and photocatalytic purification, which can be applied for improving air quality and human health.

Circ_0000467 regulates proliferation, migration, invasion, and apoptosis in gastric cancer by targeting the miR-622/ROCK2 axis.

BACKGROUND: Gastric cancer (GC) ranks fourth as a cause of cancer-induced mortality worldwide. Recently, some studies have demonstrated that circular RNAs (circRNAs) play vital roles in human cancers, including GC. METHODS: The expression levels of circ_0000467, microRNA-622 (miR-622), and Rho-associated coiled-coil-containing protein kinase2 (ROCK2) were determined by RT-qPCR assay. The protein expression was quantified by western blot assay. The interaction relationship between miR-622 and circ_0000467 or ROCK2 was confirmed by dual-luciferase reporter assay and RIP assay. The biological behaviors of GC cells including proliferation, apoptosis, migration, and invasion were determined by EdU assay, colony-forming assay, flow cytometry, and transwell assay. The effects of circ_0000467 silencing in vivo were assessed by a xenograft experiment in nude mice. RESULTS: MiR-622 was downregulated and ROCK2 was upregulated in GC tissues and cells. Loss-of-function experiment revealed that overexpression of miR-622 decreased proliferation, migration, and invasion while it increased apoptosis in GC cells. Furthermore, ROCK2 was a functional target of miR-622, and upregulation of ROCK2 abolished miR-622-induced effects on GC cells. What's more, circ_0000467 was upregulated in GC, and inhibition of miR-622 reversed silencing of circ_0000467-caused effects on GC cells, suggesting that miR-622 was a target of circ_0000467. The suppression of circ_0000467 was able to slow the tumor growth in vivo. CONCLUSION: Mechanistically, circ_0000467 functioned as an oncogenic regulator in GC by specifically binding to miR-622 to upregulate ROCK2, which might be novel diagnostic markers for GC.

Membrane Applications in Autologous Cell Therapy.

Stem cell and cell therapies, particularly autologous cell therapies, are becoming a common practice. However, in order for these technologies to achieve wide-scale clinical application, the prohibitively high cost associated with these therapies must be addressed through creative engineering. Membranes can be a disruptive technology to reshape the bioprocessing and manufacture of cellular products and significantly reduce the cost of autologous cell therapies. Examples of successful membrane applications include expansions of CAR-T cells, various human stem cells, and production of extracellular vesicles (EVs) using hollow fibre membrane bioreactors. Novel membranes with tailored functions and surface properties and novel membrane modules that can accommodate the changing needs for surface area and transport properties are to be developed to fulfil this key role.

Bacillus suaedae sp. nov., isolated from the stem of Suaeda aralocaspica in north-west China.

A bacterial strain, designated YZJH907-2T, was isolated from the stem of Suaeda aralocaspica, collected from the southern edge of the Gurbantunggut desert, Xinjiang, PR China. Cells of strain YZJH907-2T were Gram-stain-positive, aerobic and rod-shaped. They formed white or colourless circular colonies with smooth convex surfaces. Strain YZJH907-2T grew at 4-50 °C (optimum, 28-30 °C), pH 7.0-10.0 (optimum, pH 8.0-9.0) and with 0-10 % (w/v) NaCl (optimum, 3-7 %). The genomic DNA G+C content of strain YZJH907-2T was 38.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that the strain was most closely related to Bacillus alcalophilus DSM 485T (97.37 %), Bacillus kiskunsagensis B16-24T (96.87 %) and Bacillus bogoriensis LBB3T (96.71 %). Average nucleotide identity values between YZJH907-2T and B. alcalophilus DSM 485Tand B. bogoriensis LBB3T were 69.2 and 69.0 %, respectively. Digital DNA-DNA hybridization values of YZJH907-2T with B. alcalophilus DSM 485T and B. bogoriensis LBB3T were 19.6 and 20.4 %, respectively. The cell wall of strain YZJH907-2T contained meso-diaminopimelic acid, and the major and secondary isoprenoid quinones were MK-7 and MK-5, respectively. Results of fatty acids showed that anteiso-C15 : 0, iso-C15 : 0 and C16 : 0 were the predominant cellular fatty acids. Two-dimensional thin-layer chromatography analysis indicated that the polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified phospholipids and two unidentified glycolipids. Based on the genomic, phylogenetic and phenotypic analyses, strain YZJH907-2T represented a novel species of the genus Bacillus, and thus the name Bacillus suaedae sp. nov. is proposed. The type strain is YZJH907-2T (=CGMCC 1.18763T=KCTC 43335T).

Cranio-maxillofacial post-operative face prediction by deep spatial multiband VGG-NET CNN.

Current plastic and reconstructive surgery computational techniques are not precise and take a long time to perform. Therefore, these limitations reduced the adoption of computational techniques. Although computer-aided surgical preparation systems may help to enhance clinical results, minimize operating time and costs, they are too complicated and require detailed manual information, which restricts their usage in doctor-patient communication and clinical decision-making. In order to obtain the optimal aesthetic and reconstruction treatment results, these techniques must be designed and implemented carefully. Computer-aided modeling, planning, and simulation techniques enable the preoperational evaluation of various therapeutic strategies based on the 3D patient models. We offer the new deep-learning architecture for diagnostics, risk stratification, and post-operative simulation for face prediction. Initially, preprocessing was done by using the weighted adaptive median filter and Laplacian partial differential equation-based histogram equalization. Then the target area was converted to 3D for clear visualization by using the Smart restorative frustum model. Finally, the post-operative face prediction was constructed by using the deep spatial Multiband VGG NET CNN. We obtained a face dataset of 313,318 CT and their clinical records from different centers. The algorithms were developed by 21,095 scans (Qure25k data set). In addition, CQ500 datasets from various centers were compiled in two batches, B1 and B2, to validate the algorithms clinically. Four hundred ninety-one scans used the CQ500 dataset. Initially, we reconstructed the input image and then devised the post-operative face computationally. The suggested deep spatial Multiband VGG NET CNN showed the high range of post-operative face prediction accuracy. Therefore, successful metrics such as the Jaccard and dice scores have shown accurate outcomes compared to other traditional methods. MATLAB was used to obtain the output of proposed work. With the help of the suggested classifier, the prediction accuracy was 93.7%, sensitivity was 99.9%, and specificity was 99.8%, all of which were higher than traditional approaches. Here, the suggested method provides better results for post-operative face prediction to the applied dataset than any other existing mechanisms. It is a generalized attempt that can apply to other similar datasets as well.

Isometric Double-Layer Staggered Chain Teeth Triboelectric Nanogenerator.

The sliding freestanding layer triboelectric nanogenerator (SF-TENG) is a sustainable power source that can convert mechanical energy from linear or rotating mechanical motion to electrical energy. This paper proposes a double-layer staggered chain teeth TENG. Comparing the staggered electrode TENG and the double-layer staggered electrode TENG, the output voltage difference is relatively small. The electrode of the TENG is designed to the shape of chain teeth, which proves that TENG can be combined with a zipper, and the best distance among chain teeth in the TENG is determined through experiments. Compared with traditional zippers, the double-layer staggered chain teeth TENG can generate electrical energy during the continuous pulling of the zipper. The double-layer staggered chain teeth TENG has good performance. When the external load is 20 MΩ, the maximum output power reaches 20.18 µW. After the rectification and transformation, the generated electricity can light up 30 LED lights or more, and can also supply power to electronic devices. Through the chain teeth array, the open circuit voltage and transfer charge generated by the zipper during the continuous pulling process are improved. The double-layer staggered chain teeth TENG has a good usage environment in life, and this work will provide valuable insights for the development of SF-TENG technology.

Fish-Wearable Data Snooping Platform for Underwater Energy Harvesting and Fish Behavior Monitoring.

Conventional approaches to studying fish kinematics pose a great challenge for the real-time monitoring of fish motion kinematics. Here, a multifunctional fish-wearable data snooping platform (FDSP) for studying fish kinematics is demonstrated based on an air sac triboelectric nanogenerator (AS-TENG) with antibacterial coating. The AS-TENG not only can harvest energy from fish swimming but also serves as the self-powered sensory module to monitor the swimming behavior of the fish. The peak output power generated from each swing of the fishtail can reach 0.74 mW, while its output voltage can reflect the real-time behavior of the fishtail. The antibacterial coating on the FDSP can improve its biocompatibility and the elastic texture of the FDSP allows it to be tightly attached to fish. The wireless communication system is designed to transmit the sensory data to a cell phone, where the detailed parameters of fish motion can be obtained, including swing angle, swing frequency, and even the typical swing gestures. This FDSP has broad application prospects in underwater self-powered sensors, wearable tracking devices, and soft robots.

Pontibacter pamirensis sp. nov., isolated from saline-alkaline soil.

A novel bacterium, designated TRT317T, was isolated from saline-alkaline soil collected from the Pamir plateau in northwest China. Cells of this strain were Gram-stain-negative, aerobic rods and red-pink-coloured. Phylogenetic analysis using 16S rRNA gene sequences indicated that strain TRT317T showed the highest sequence similarity to the type strains of Pontibacter diazotrophicus (96.3 %) and Pontibacter yuliensis (96.2 %). Growth was observed at 4-40 °C, pH 6.0-10.0 and in the presence of up to 7 % (w/v) NaCl. The major fatty acids were iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I/anteiso-C17 : 1 B). The polar lipids included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified phospholipid, four unidentified glycolipids and five unidentified lipids. The whole-cell sugars of strain TRT317T were mannose, rhamnose, glucose, galactose, xylose, arabinose and four unidentified sugars. The sole respiratory quinone was MK-7. The genomic DNA G+C content of strain TRT317T was 47.7 mol%. The average nucleotide identity (ANI) value of strain TRT317T with P. diazotrophicus was 88.3 %, which is below the standard ANI threshold for species identification (95-96 %). Combined results of physiological, genotypic, phylogenetic and chemotaxonomic analyses demonstrated that strain TRT317T represents a novel species within the genus Pontibacter, for which the name Pontibacter pamirensis sp. nov. is proposed. The type strain is TRT317T (=CGMCC1.18690T=KCTC 82818T).

Sanguibacter suaedae sp. nov., isolated from the root of Suaeda aralocaspica in north-west PR China.

A bacterial strain, designated YZGR15T, was isolated from the root of an annual halophyte Suaeda aralocaspica, collected from the southern edge of the Gurbantunggut desert, north-west PR China. Cells of the isolate were Gram-stain-positive, facultatively anaerobic, irregular rods. Growth occurred at 4-42 °C (optimum, 30-37 °C), at pH 6.0-9.0 (optimum, pH 7.0-7.5) and in the presence of 0-9 % (w/v) NaCl (optimum, 2-5 %). Phylogenetic analysis using 16S rRNA gene sequences indicated that strain YZGR15T showed the highest sequence similarity to Sanguibacter keddieii (98.27 %), Sanguibacter antarcticus (98.20 %) and Sanguibacter inulinus (98.06 %). Results of genome analyses of strain YZGR15T indicated that the genome size was 3.16 Mb, with a genomic DNA G+C content of 71.9 mol%. Average nucleotide identity and digital DNA-DNA hybridization values between strain YZGR15Tand three type strains were in the range of 76.5-77.8 % and 20.0-22.2 %, respectively. Analysis of the cellular component of strain YZGR15T revealed that the primary fatty acids were anteiso-C15 : 0, C16 : 0, C14 : 0 and iso-C16 : 0 and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids and two unidentified glycolipids. The cell-wall characteristic amino acids were glutamic acid, alanine and an unknown amino acid. The whole-cell sugars for the strain were mannose, ribose, rhamnose, glucose and an unidentified sugar. The predominant respiratory quinone was MK-9(H4). Based on the results of genomic, phylogenetic, phenotypic and chemotaxonomic analyses, strain YZGR15T represents a novel species of the genus Sanguibacter, for which the name Sanguibacter suaedae sp. nov. is proposed. The type strain is YZGR15T (=CGMCC 1.18691T=KCTC 49659T).

Self-Powered Room-Temperature Ethanol Sensor Based on Brush-Shaped Triboelectric Nanogenerator.

Highly sensitive ethanol sensors have been widely utilized in environmental protection, industrial monitoring, and drink-driving tests. In this work, a fully self-powered ethanol detector operating at room temperature has been developed based on a triboelectric nanogenerator (TENG). The gas-sensitive oxide semiconductor is selected as the sensory component for the ethanol detection, while the resistance change of the oxide semiconductor can well match the "linear" region of the load characteristic curve of TENG. Hence, the output signal of TENG can directly reveal the concentration change of ethanol gas. An accelerator gearbox is applied to support the operation of the TENG, and the concentration change of ethanol gas can be visualized on the Liquid Crystal Display. This fully self-powered ethanol detector has excellent durability, low fabrication cost, and high selectivity of 5 ppm. Therefore, the ethanol detector based on TENG not only provides a different approach for the gas detection but also further demonstrates the application potential of TENG for various sensory devices.

Adipose-derived stem cell sheets combined with ß-tricalcium phosphate/collagen-I fiber scaffold improve cell osteogenesis.

Transplantation of cell-based material is a promising approach for the treatment of critical bone defects. However, it is still limited by the lack of suitable scaffold material or abundant seeding cell sources. The present study aimed to establish a novel composite of an adipose-derived stem cell (ADSC) sheet and a synthetic porous ß-tricalcium phosphate/collagen-I fiber (ß-TCP/COL-I) scaffold to enhance osteogenic activity. ADSCs were isolated from 3-week-old female Sprague Dawley rats and the ADSC sheets were prepared in an osteoinductive medium. The study groups included the ADSC sheets/scaffold, scattered ADSCs/scaffold, ADSC sheet alone and scaffold alone. Scanning electron microscopy and energy-dispersive spectrometry were used to observe cell-scaffold interactions and analyze the relative calcium content on the composites' surface. Alizarin red S staining was used to examine the calcium deposition. ELISA and reverse transcription-quantitative PCR were used to detect the expression levels of alkaline phosphatase (ALP), osteocalcin (OCN) and osteopontin (OPN). The results revealed that ADSCs were able to tightly adhere to the ß-TCP/COL-I scaffold with no cytotoxicity. The calcifying nodules reaction was positive on ADSC sheets and gradually increased after osteogenic induction. In addition, the ß-TCP/COL-I scaffold combined with ADSC sheets was able to significantly enhance the expression levels of ALP, OCN and OPN and increase the superficial relative calcium content compared to scattered ADSCs/scaffold or the ADSC sheet alone (P<0.05). The results indicated that ADSCs possess a strong osteogenic potential, particularly in the cell-sheet form and when compounded with the ß-TCP/COL-I scaffold, compared to scattered ADSCs with a ß-TCP/COL-I scaffold or an ADSC sheet alone. This novel composite may be a promising candidate for bone engineering.