[Immobilization of EcoR1, Pae1 and Lpl1 restriction enzymes]. / Immobilizatsiia restriktaz EcoR1, Pae1 i Lpl1]

In search for sorbents (silica gels, styrene-divinylbenzene copolymers), for immobilization of some restriction endonucleases, derivatives of trityl-containing silochroms are shown to bind EcoRI, PaeI and LplI endonucleases with the retention of 10-20, 60-70 and 40-60% activity, respectively. The immobilized restriction endonucleases have the unchanged substrate specificity, can be used several times and are stable at storage. Tritylaminopropylsilochrom is suggested to be the sorbent of choice.

[Ion-exchange hemosorption in the intensive therapy of liver insufficiency in patients with obstructive jaundice]. / Ionoobmennaia gemosorbtsiia v intensivnoi terapii pechenochnoi nedostatochnosti u bol'nykh obturatsionnoi zheltukhi.

Hemosorption on thromboresistant ion-exchange resins synthetized at D. I. Mendeleev Moscow Chemical Technological Institute (MCTI) was used in combined therapy of hepatic failure. Use was made of anion-exchange resin A-I-II MCTI, catonit C-I-II MCTI, polyampholit. Stability of hemodynamic parameters, absence of blood element disturbances, effective sampling of anionic and cationic metabolites have been observed.