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1.
Bioorg Khim ; 18(2): 210-6, 1992 Feb.
Article in Russian | MEDLINE | ID: mdl-1534987

ABSTRACT

In search for sorbents (silica gels, styrene-divinylbenzene copolymers), for immobilization of some restriction endonucleases, derivatives of trityl-containing silochroms are shown to bind EcoRI, PaeI and LplI endonucleases with the retention of 10-20, 60-70 and 40-60% activity, respectively. The immobilized restriction endonucleases have the unchanged substrate specificity, can be used several times and are stable at storage. Tritylaminopropylsilochrom is suggested to be the sorbent of choice.


Subject(s)
Deoxyribonuclease EcoRI/metabolism , Deoxyribonucleases, Type II Site-Specific/metabolism , Enzymes, Immobilized , Bacteriophage lambda/metabolism , DNA, Viral/metabolism
3.
Anesteziol Reanimatol ; (4): 40-2, 1989.
Article in Russian | MEDLINE | ID: mdl-2817498

ABSTRACT

Hemosorption on thromboresistant ion-exchange resins synthetized at D. I. Mendeleev Moscow Chemical Technological Institute (MCTI) was used in combined therapy of hepatic failure. Use was made of anion-exchange resin A-I-II MCTI, catonit C-I-II MCTI, polyampholit. Stability of hemodynamic parameters, absence of blood element disturbances, effective sampling of anionic and cationic metabolites have been observed.


Subject(s)
Cholestasis/therapy , Critical Care/methods , Hemoperfusion , Ion Exchange Resins/therapeutic use , Liver Diseases/therapy , Aged , Aged, 80 and over , Humans , Middle Aged
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