[Effect of different types of interferon on the natural cellular cytotoxicity of lung cancer patients]. / Vliianie razlichnykh tipov interferona na estestvennuiu kletochnuiu tsitotoksichnost' u bol'nykh rakom legkikh.

The natural killer cell cytotoxicity was studied in the 4 hour and 18 hour cytotoxic reaction with cultured human tumour cells (K562 and lung adenocarcinoma) labelled by 51Cr. The natural killer cell activity of cancer patients was lowered only in 4 hour cytotoxic reaction with target cells K562. The natural and recombinant leucocyte interferon and natural immune interferon stimulated in vitro the activity of natural killer cells in most of the experiments (80.5 %) in the 4 hour and 18 hour cytotoxic reactions both from patients with initially lowered level, and with normal or high level of cytotoxic action of natural killer cells. In some experiments interferon preparations did not significantly influence the lysis of tumour cells or even suppressed the activity of effector cells. The heterogeneity of the population of natural killer cells and different immunomodulating effects of interferon were discussed.

[Cytotoxic action of natural killer cells on human tumor cells]. / Tsitotoksicheskoe deistvie estestvennykh kletok-killerov na opukholevye kletki cheloveka.

Natural killer cell cytotoxicity was studied in a 18-hour 51Cr-release assay in the cultures of human tumor target cells: K562 leukemia and lung adenocarcinoma (LAC) cells. The mean cytotoxic value was similar for K562 and LAC cells: 36.13 +/- 3.23% and 40.78 +/- 3.43%, respectively, although significant individual variability was recorded. The similar cytolytic action of blood mononuclear cells (MNC) on the two tumor lines was observed in 30% of normal donors. MNC from 30% donors produced more pronounced lytic action on K562 cells while MNC from other 30% donors lysed mainly LAC cells. In the competitive inhibition test cold K562 cells more effectively than cold LAC cells suppressed the MNC-induced lysis of both K562 and LAC cells.