ABSTRACT
We investigated the global placental gene expression profile in severe preeclampsia. Twenty-one women were randomly selected from 50 participants with uncomplicated pregnancies to match 21 patients with severe preeclampsia. A 30K Human Genome Survey Microarray v.2.0 (Applied Biosystems) was used to evaluate the gene expression profile. After RNA isolation, five preeclamptic placentas were excluded due to poor RNA quality. The series composed of 37 hybridizations in a one-channel detection system of chemiluminescence emitted by the microarrays. An empirical Bayes analysis was applied to find differentially expressed genes. In preeclamptic placentas 213 genes were significantly (fold-change>or=2 and pSubject(s)
Gene Expression Regulation, Developmental
, Placenta/metabolism
, Pre-Eclampsia/genetics
, Adult
, Angiogenesis Inducing Agents/metabolism
, Chorionic Gonadotropin/genetics
, Down-Regulation
, Female
, Gene Expression Profiling
, Humans
, Leptin/genetics
, Metalloproteases/genetics
, Oligonucleotide Array Sequence Analysis
, Parity
, Pre-Eclampsia/metabolism
, Pregnancy
, Receptors, Notch/genetics
, Reverse Transcriptase Polymerase Chain Reaction
, Up-Regulation