In vitro quality parameters of whole blood-derived platelets pooled using two different platelet pooling sets and stored up to 7 days are similar.

BACKGROUND: Increasing the number of collections of whole blood-derived platelets (WBDP) and lengthening the allowable storage time may alleviate platelet (PLT) shortages. There is a need for new PLT pooling sets that can provide acceptable quality on Day 7 of storage. STUDY DESIGN AND METHODS: This pool-and-split study compared WBDP prepared using the platelet-rich plasma method with the novel IMUGARD WB PLT pooling set and a control pooling set. After pooling and filtration, PLT products were tested on Days 1, 5, and 7. Large volume delayed sampling (LVDS) cultures were taken on Day 2. RESULTS: The median postfiltration residual white blood cell (rWBC) content was 0.18 million per product (maximum 1.26 million; n = 69) with mean PLT recovery of 88.5 ± 2.8% for the new set and median 0.23 million (maximum 1.83 million) rWBC with 87.5 ± 2.5% recovery for the control. Day 5 mean pH22°C were 7.18 ± 0.12 and 7.13 ± 0.10 for the new and control set, respectively. Day 5 in vitro quality parameters were within 20% between the two pooling sets. The new set Day 7 pH22°C was acceptable (7.07 ± 0.17, 100% ≥ 6.3), and most parameters were within 20% of Day 5 values. CONCLUSION: WBDP quality for the new pooling set is acceptable across a battery of in vitro tests when stored up to 7 days and meets FDA regulatory criteria. The quality parameters were similar between the new pooling set and the control set on Day 5. This new set is compatible with LVDS.

Feasibility evaluation of two novel systems for the automated preparation and extended storage of DMSO cryopreserved platelets.

BACKGROUND: Manufacturing methods for dimethyl sulfoxide (DMSO)-cryopreserved platelets (CPPs) are manual and labor intensive. Thawing and prepare-for-transfusion steps are in an open system that requires transfusion within 4 h. A fill-and-finish system (CUE) can automate the manufacturing process. A newly configured bag system allows freezing, thawing, and use of resuspension solutions while maintaining the functionally closed system, and extending the post-thaw shelf life beyond 4 h. Our objective is to evaluate the feasibility of the CUE system and the functionally closed bag system. STUDY DESIGN AND METHODS: DMSO was volumetrically added to double-dose apheresis platelets, concentrated, and delivered to a 50- or 500-mL ethylene-vinyl acetate (EVA) bag by the CUE (n = 12). The functionally closed bag system contained 25 mL platelet additive solution 3 (PAS-3) in a 50-mL EVA bag. Control CPP (n = 2) were manually prepared. PAS-3 and CPP were thawed together. CPP were stored up to 98 h (20-24°C) and tested using a standard assay panel. RESULTS: CUE prepared CPP met the design targets: volume, platelet content, and DMSO concentration. CUE CPP P-selectin was high. CD42b, phosphatidylserine (PS) expression, and live cell percentage were favorable compared to controls and favorably maintained over storage. The thrombin generation potency was slightly reduced compared to controls. The 50 mL EVA bag maintained pH for up to 30 h, and the 500 mL EVA bag beyond 76 h. DISCUSSION: The CUE system presents a technically feasible method to prepare CPP. A functionally closed bag system with resuspension solution was successful and can extend the post-thaw storage time of CPP.

Percepção dos enfermeiros sobre o uso da caderneta de saúde da pessoa idosa na atenção primária / Perception of nurses on the use of the health booklet of the elderly in primary care / Percepción de los enfermeros sobre la utilización de la cartilla sanitaria del anciano en atención primaria

Introdução: A Atenção Primária à Saúde capta as necessidades de saúde da população, tem a responsabilidade de realizar ações de promoção e proteção à saúde, prevenção de agravos, reabilitação, redução de danos e manutenção da saúde, estratificando os riscos e direcionando os cuidados para outros pontos da rede. Tendo em vista o crescimento progressivo da população idosa, as equipes de Atenção Primária à Saúde têm papel fundamental no desenvolvimento de ações de abordagem integral à saúde deste grupo, aplicando instrumentos como a caderneta de saúde da pessoa idosa, desenvolvida para potencializar o acompanhamento de saúde dos indivíduos a partir dos 60 anos. Objetivo: Identificar a percepção da utilização da caderneta de saúde da pessoa idosa pelos enfermeiros em unidades de Atenção Primária à Saúde. Materiais: Participaram do estudo seis enfermeiros um de cada uma das unidades escolhidas. Métodos: Trata-se de um estudo descritivo e exploratório com abordagem qualitativa. Coletou-se dados através de entrevista semiestruturada, com gravação de áudio, no ambiente de trabalho dos enfermeiros no mês de agosto de 2022, com duração média de 10 a 20 minutos, realizada em seis unidades de Atenção Primária de um munícipio do oeste do Paraná, sendo três unidades básicas de saúde e três unidades de saúde da família. Resultados e Discussão: Foram entrevistados seis enfermeiros ambos do sexo feminino e a idade varia de 30 a 56 anos, com atuação na unidade que corresponde a pesquisa de três a cinco anos. 66,6 % dos enfermeiros conheciam a caderneta de saúde da pessoa idosa e 33,3% não tiveram acesso a esta ferramenta sendo que nenhum dos enfermeiros entrevistados a utiliza, mesmo atuando na Atenção Primaria à Saúde há mais de 10 anos. Considerações finais: Apesar das enfermeiras reconhecerem a importância deste instrumento para o cuidado da saúde do idoso, dando a ele e a família mais conhecimento sobre seu estado de saúde, elas encontram vários obstáculos para implantá-la de modo satisfatório, tanto para equipe quanto para o idoso.

Introduction: Primary Health Care captures the health needs of the population, has the responsibility to perform actions to promote and protect health, prevent diseases, rehabilitation, harm reduction and health maintenance, stratifying the risks and directing care to other points in the network. Considering the progressive growth of the elderly population, the Primary Health Care teams have a fundamental role in the development of actions of integral approach to the health of this group, applying tools such as the health booklet of the elderly, developed to enhance the health monitoring of individuals over 60 years. Objective: To identify the perception of the use of the health booklet for the elderly by nurses in Primary Health Care units. Materials: Six nurses participated in the study, one from each of the chosen units. Methods: This is a descriptive and exploratory study with a qualitative approach. Data were collected through semi- structured interviews, with audio recording, in the nurses' work environment in August 2022, with an average duration of 10 to 20 minutes, carried out in six units of Primary Care in a city in western Paraná, three basic health units and three family health units. Results and Discussion: Six nurses were interviewed, both female and ranging in age from 30 to 56 years old, working in the unit that corresponds to the research from three to five years. 66.6% of the nurses knew the health booklet for the elderly and 33.3% did not have access to this tool. None of the interviewed nurses uses it, even though they have been working in Primary Health Care for more than 10 years. Final considerations: Although nurses recognize the importance of this tool for elderly people's health care, giving them and their families more knowledge about their health status, they face several obstacles to implement it in a satisfactory way, both for the team and for the elderly.

Introducción: La Atención Primaria de Salud capta las necesidades de salud de la población, tiene la responsabilidad de realizar acciones de promoción y protección de la salud, prevención de enfermedades, rehabilitación, reducción de daños y mantenimiento de la salud, estratificando los riesgos y dirigiendo los cuidados a otros puntos de la red. Dado el progresivo crecimiento de la población anciana, los equipos de Atención Primaria de Salud tienen un papel clave en el desarrollo de acciones de abordaje integral de la salud de este colectivo, aplicando herramientas como la cartilla de salud del anciano, desarrollada para potenciar el seguimiento de la salud de los individuos a partir de los 60 años. Objetivo: Identificar a percepção da utilização da cartilla de saúde da pessoa idosa pelos enfermeiros em unidades de Atenção Primária à Saúde. Materiais: Participaram do estudo seis enfermeiros um de cada uma das unidades escolhidas. Método: Este é um estudo descriptivo e exploratório com abordagem qualitativa. Los datos fueron recolectados a través de entrevistas semiestructuradas, con grabación de audio, en el ambiente de trabajo de enfermeros en agosto de 2022, con una duración media de 10 a 20 minutos, realizadas en seis unidades de Atención Primaria de un municipio del oeste de Paraná, tres unidades básicas de salud y tres unidades de salud familiar. Resultados y Discusión: Fueron entrevistados seis enfermeros, ambos del sexo femenino, edad entre 30 y 56 años, trabajando en la unidad correspondiente a la investigación de tres a cinco años. El 66,6% de las enfermeras conocían la cartilla de salud del anciano y el 33,3% no tenían acceso a esta herramienta y ninguna de las enfermeras entrevistadas

Violência na Estratégia de Saúde da Família: riscos para a saúde dos trabalhadores e ao atendimento / Violence in the Family Health Strategy: risks to workers' health and care / Violencia en la estrategia de salud de la familia: riesgos para la salud de los trabajadores y la atención

Objetivo: identificar a ocorrência da violência na Estratégia de Saúde da Família e analisar as repercussões para a saúde dos trabalhadores e ao atendimento. Método: estudo qualitativo, descritivo, realizado com 27 trabalhadores de uma unidade de Estratégia de Saúde da Família localizada no município do Rio de Janeiro em 2018. Trabalhou-se com a técnica de entrevista semiestruturada mediante roteiro e para a categorização dos depoimentos a análise de conteúdo temática. Projeto de pesquisa aprovado pelo Comitê de Ética em Pesquisa. Resultados: identificou-se a violência psicológica perpetrada por usuários através de ameaças, xingamentos e intimidações e a urbana com a exposição dos profissionais a tiros, assaltos e brigas durante as visitas domiciliares ocasionando repercussões na saúde mental dos trabalhadores e prejuízos para a qualidade do atendimento. Conclusão: a violência ocupacional é um risco a integridade física e psíquica dos trabalhadores e a qualidade do atendimento.

Objective: to identify the occurrence of violence in Brazil's Family Health Strategy and to analyze the repercussions on workers' health and on care. Method: this qualitative, descriptive study was conducted in 2018 with 27 workers from a Family Health Strategy unit in the city of Rio de Janeiro, using the semi-structured, scripted interview technique, and thematic content analysis to categorize interviewee discourse. The project was approved by the research ethics committee. Results: the violence identified was psychological, as perpetrated by users in threats, name calling and intimidation, and urban, in health personnel's exposure to gunfire, robbery, and fights during home visits, which had repercussions on the workers' mental health and impaired the quality of care. Conclusion: occupational violence is a risk to health workers' physical and mental integrity and to the quality of care.

Objetivo: identificar la ocurrencia de violencia en la Estrategia de Salud de la Familia de Brasil y analizar las repercusiones en la salud y la atención de los trabajadores. Método: este estudio cualitativo descriptivo se realizó en 2018 con 27 trabajadores de una unidad de Estrategia de Salud de la Familia en la ciudad de Río de Janeiro, utilizando la técnica de entrevista semiestructurada, guionizada y análisis de contenido temático para categorizar el discurso del entrevistado. El proyecto fue aprobado por el comité de ética en investigación. Resultados: la violencia identificada fue psicológica, perpetrada por los usuarios en amenazas, insultos e intimidación, y urbana, en la exposición del personal de salud a disparos, robos y peleas durante las visitas domiciliarias, lo que repercutió en la salud mental de los trabajadores y perjudicó la calidad de atención. Conclusión: la violencia laboral es un riesgo para la integridad física y mental de los trabajadores de la salud y para la calidad de la atención.

Trabalhando no território: violência relacionada ao trabalho da equipe de saúde da Estratégia de Saúde da Família / Working in the territory: violence related to the work of the health team of the Family Health Strategy

O presente estudo tem como objeto a "violência relacionada ao trabalho da equipe de saúde da Estratégia de Saúde da Família e as implicações para a saúde dos trabalhadores". Os objetivos do estudo foram: identificar os tipos de violência relacionados ao trabalho na ESF, na visão dos trabalhadores da saúde; descrever as repercussões da violência relacionadas ao trabalho para a saúde dos trabalhadores e analisar as estratégias de enfrentamento adotadas pelos trabalhadores. Estudo qualitativo do tipo descritivo e exploratório. O campo foi uma unidade de ESF da Área Programática 3.2, localizada no município do Rio de Janeiro. Participaram do estudo 27 trabalhadores, sendo: 13 agentes comunitários de saúde, 2 auxiliares de saúde bucal, 3 enfermeiros, 4 médicos, 4 técnicos de enfermagem, 1 técnico de saúde bucal. Na coleta dos depoimentos, trabalhou-se com a técnica de entrevista, mediante um roteiro e, na caracterização sociodemográfica e ocupacional, adotou-se um instrumento estruturado. As entrevistas foram realizadas na própria unidade e gravadas após a autorização do estudo pelo Comitê de Ética em Pesquisa da Universidade do Estado do Rio de Janeiro ­ CEP/UERJ nº 2.509.816 ­, pela Secretaria Municipal de Saúde ­ CEP/SMS-RJ nº 2.668.601­, e pela assinatura do Termo de Consentimento Livre e Esclarecido. Após a transcrição dos depoimentos na íntegra, aplicou-se a técnica de análise de conteúdo temática, cujos resultados foram discutidos à luz da psicodinâmica do trabalho. Sobre os tipos de violência, identificou-se, principalmente a psicológica perpetrada pelos usuários, através de agressão verbal, intimidações e ameaças, além da violência no território devido à exposição dos trabalhadores a tiroteios, tráfico de drogas e assaltos. Acerca das repercussões da violência para a saúde dos trabalhadores, identificou-se o sofrimento psíquico através das queixas de medo, insegurança, estresse, nervosismo e tensão que, além de repercutirem na saúde dos trabalhadores, podem levar à insatisfação, desmotivação e, até mesmo, à desistência do trabalho como estratégia de manutenção da saúde. Frente à violência, os trabalhadores elaboram estratégias de defesa como o silêncio, a resignação, a banalização e o modus operandi mediante utilização de dispositivo de acesso seguro. Além das estratégias referidas, houve sugestões de suporte psicoterápico aos trabalhadores, realização de grupos de autoajuda e adoção de medidas de segurança na unidade de atendimento. Concluiu-se que a violência relacionada ao trabalho é um risco psicossocial que afeta a saúde dos trabalhadores, cabendo à organização do trabalho, em conjunto com esses profissionais, a elaboração de medidas que proporcionem a visibilidade dessa problemática, bem como estratégias de prevenção e enfrentamento coletivo. Ratifica-se a importância do apoio psicoterápico aos trabalhadores vítimas de violência e o fortalecimento dos fatores de proteção. Recomenda-se a continuidade de estudos desta natureza envolvendo outras unidades e um maior número de participantes, de modo a proporcionar maior visibilidade à violência que afeta a saúde dos trabalhadores e a qualidade das atividades de cunho assistencial e educativo à população local.

The present study has as its object the "violence related to the work of the health team of the Family Health Strategy (FHS) and its implications for the health of employees". The objectives of the study were: to identify the types of work-related violence in the FHS in the vision of health workers; describe the effects of work-related violence to workers' health and to analyze the coping strategies adopted by workers. Qualitative study of descriptive and exploratory type. The study field was a unit of FHS at the programmatic area 3.2, located in the city of Rio de Janeiro. Twenty-seven workers participated in the study: 13 community health agents, 2 oral health assistants, 3 nurses, 4 doctors, 4 nursing technicians, 1 dental health technician. In the collection of testimonies, we worked with the interview technique, using a script, and in order to analyze the demographic and occupational profiling a structured instrument was adopted. The interviews were conducted in the unit itself and recorded after the authorization for the study by the Research Ethics Committee of the Rio de Janeiro State University ­ CEP/UERJ nº 2.509.816 ­ and by the Municipal Health Secretariat ­ CEP/SMS- RJ n. 2.668.601­, and after signature of the informed consent (TFCC). After the transcription of the testimonies in its entirety, the technique of thematic content analysis was applied, the results of which were discussed in the light of the psychodynamics of work. Regarding the types of violence, the main one identified was the psychological violence perpetrated by users through verbal aggression, intimidation and threats and territorial violence due to the workers' exposure to drive-by shootings, drug trafficking and robberies. With respect to the repercussions of violence for the workers' health, psychic suffering was identified through their complaints of fear, insecurity, stress, nervousness and tension which, in addition to an impact on the workers' health, may lead to dissatisfaction, discouragement and even withdrawal from work as a health maintenance strategy. Faced with the violence, the workers develop defense strategies such as silence, resignation, trivialization and the modus operandi using secure access device. In addition to the mentioned strategies there were suggestions of psychotherapeutic support to workers, promotion of self-help groups, and adoption of security measures in the care unit. It was concluded that violence related to work is a psychosocial risk that affects the workers' health, and that the organization of work, in conjunction with these professionals, shall draw up measures which provide visibility of this issue, as well as develop prevention and coping strategies. The study verifies the importance of psychotherapeutic support to workers who are victims of violence and the strengthening of protection factors. It is recommended the continuation of studies of this nature involving other units and a greater number of participants in order to provide greater visibility to the violence that affects the workers' health as well as the quality of assistance and education measures provided to the local population.

Ptose miogênica na distrofia muscular oculofaríngea / Miogenic ptosis in oculopharyngeal muscular dystrophy

RESUMO Relato de caso de distrofia muscular oculofaríngea, doença genética de herança autossômica dominante e uma das causas de ptose miogênica adquirida. A paciente apresentou quadro de ptose palpebral bilateral e disfagia, achados clínicos típicos da doença, foi submetida a tratamento cirúrgico da ptose, com bom resultado estético e funcional.

ABSTRACT The authors report a case of oculopharyngeal muscular dystrophy, an autosomal dominant genetic disease, which leads to miogenic ptosis. This patient presented bilateral palpebral ptosis and dysphagia and underwent ptosis surgical treatment, with a good functional and aesthetic result.

Inactivation of Escherichia coli, Listeria monocytogenes, and Salmonella Enteritidis by Cymbopogon citratus D.C. Stapf. Essential Oil in Pineapple Juice.

In the present study, the efficacy of Cymbopogon citratus D.C. Stapf. essential oil (CCEO) to provoke a 5-log CFU/ml (5-log) inactivation in a mixed composite of Escherichia coli, Listeria monocytogenes, and Salmonella enterica serovar Enteritidis in pineapple (Ananas comosus (L.) Merril) juice (4°C) was assessed. Moreover, the effects of CCEO on the physicochemical and sensory quality parameters of pineapple juice were evaluated. The MIC of CCEO was 5 µl/ml against the composite mix examined. For L. monocytogenes and E. coli inoculated in juice containing CCEO (5, 2.5, and 1.25 µl/ml), a ≥5-log reduction was detected after 15 min of exposure. This same result was obtained for Salmonella Enteritidis incubated alone in pineapple juice containing CCEO at 5 and 2.5 µl/ml. Overall, Salmonella Enteritidis was the most tolerant and L. monocytogenes was the most sensitive to CCEO. The physicochemical properties (pH, titratable acidic [citric acid per 100 g], and soluble solids) of pineapple juice containing CCEO (2.5 and 1.25 µl/ml) were maintained. Juice containing CCEO (2.5 and 1.25 µl/ml) exhibited similar scores for odor, appearance, and viscosity compared with juice without CCEO. However, unsatisfactory changes in taste and aftertaste were observed in juices containing CCEO. These results suggest that CCEO could be used as an alternative antimicrobial compound to ensure the safety of pineapple juice, although CCEO at the tested concentrations negatively impacted its taste. Therefore, further studies are needed to determine the balance between microbial safety and taste acceptability of pineapple juice containing CCEO.

Remanescentes: uma história de exclusão / Remnant group: a history of exclusion

O projeto de Pesquisa Intervenção “PHENIX: A Ousadia do Renascimento da Subjetividade Cidad㔠vem interagindo com adolescentes pauperizados, desde o ano 2000. O Grupo dos Remanescentes foi uma das frentes de trabalho desse projeto, formado por solicitação da coordenadora pedagógica da Instituição Pública na qual esse projeto de pesquisa intervenção se atualiza. Era composto por adolescentes demais de 18 anos de idade, a quem a instituição julgou necessário continuar a dar amparo. Com eles intervimos a partir de 2002. Em meados de 2004 ouve uma ausência dos membros do grupo às reuniões semanais. Interrompemos as atividades; realizamos entrevistas com os adolescentes para obter informações das vivências deles nesse grupo e das razões do seu afastamento. Constatamos que esses jovens, excluídos socialmente, foram impedidos de participar em algumas das atividades da instituição, tornando-se sofrido para eles lá permanecerem. Observamos, também, que a entrada no mundo do trabalho foi um dos fatores do esfacelamento do grupo. A história desse grupo aponta que, contraditoriamente aos objetivos explícitos de “reintegração social” dessa instituição, ela acompanha a sociedade numa sutil rejeição e/ou realiza a contenção/controle desses excluídos da sociedade. (AU)

Since 2000 intervention research project entitled “Phoenix: Daring in the Rebirth of Autonomous Citizenship” has been interacting with pauperized young people. The Remnant Group was one of the working groups established through the request of the pedagogical coordinator of Assistance Institution in which this research/intervention project was carried out. The group consisted of over-18-year-old young people who continued to receive help by the Institution. Intervention occurred as from 2002. However, absence of group members in weekly meeting was detected as from the mid-2004. Activities were interrupted and interviews were conducted with the young people to collect information on the daily life and the reasons for their absence. Results show that these socially excluded young people were prohibited to participate in some of the Institution’s activities and their permanence became somewhat painful. The entrance into the labor market was one of the reasons for the dismembering of the group. Its history shows that, contrary to the explicit aims of “social integration” of the institution, the group appropriate themselves of society’s subtle rejection and restrict/control marginalized young people’s access to society. (AU)

Evaluation of effects of N-(2-hydroxybenzoyl) tyramine (riparin II) from Aniba riparia (NEES) MEZ (Lauracea) in anxiety models in mice.

In order to evaluate the effects produced by N-(2-hydroxybenzoyl) tyramine (riparin II) isolated from the unripe fruit of Aniba riparia (NEES) MEZ (Lauraceae) on the central nervous system, different behavioral tests were performed. Riparin II (rip II) was administered orally (p.o.) and intraperitoneally (i.p.) in male mice, at doses of 25, 50 and 75 mg/kg, and tested on elevated plus maze (EPM), open field, rota rod and hole board tests. The results revealed that rip II caused considered increase of the number of head dips in hole board test and increased the number of entries and the time of permanence in the open arms in plus maze test in both routes. No significant effect was evidenced on rota rod and open field test, except an increase observed in the number of rearing. These results showed that riparin II presents anxiolytic-like effects in the plus maze and hole board tests which are not influenced by the locomotor activity as detected in the open field test.

Anxiolytic-like effects of (O-methyl)-N-2,6-dihydroxybenzoyl-tyramine (riparin III) from Aniba riparia (Nees) Mez (Lauraceae) in mice.

This work presents behavioral effects of (O-methyl)-N-2,6-dihydroxybenzoyl-tyramine (riparin III) isolated from the unripe fruit of Aniba riparia (Nees) Mez (Lauraceae) in animal models of open field, rota rod, elevated plus maze and hole board tests in mice. Riparin III (ripIII) was administered orally, in male mice, at single doses of 25 and 50 mg/kg. The results showed that ripIII, at both doses, had no effects on the spontaneous motor activity in the rota rod test nor in the number of squares crossed in the open field test. However, riparin III decreased the number of grooming and rearing. In the plus maze test, ripIII, at both doses increased the following parameters: percentage of entries in the open arms (PEOA), time of permanence in the open arms (TPOA) and percentage of time of permanence in the open arms (PTOA) and at the dose of 50 mg/kg, increased the number of entries in the open arms (NEOA). Similarly, ripIII, at both doses, showed an increase in the number of head dips into the holes of the hole board test. These results show that riparin III presents anxiolytic effects in the plus maze and hole board tests which are not influenced by the locomotor activity in the open field test.