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Forensic Sci Int Genet ; 2(2): 126-33, 2008 Mar.
Article in English | MEDLINE | ID: mdl-19083807

ABSTRACT

We report the results of the seventh edition of the GEP-ISFG mitochondrial DNA (mtDNA) collaborative exercise. The samples submitted to the participant laboratories were blood stains from a maternity case and simulated forensic samples, including a case of mixture. The success rate for the blood stains was moderate ( approximately 77%); even though four inexperienced laboratories concentrated about one-third of the total errors. A similar success was obtained for the analysis of mixed samples (78.8% for a hair-saliva mixture and 69.2% for a saliva-saliva mixture). Two laboratories also dissected the haplotypes contributing to the saliva-saliva mixture. Most of the errors were due to reading problems and misinterpretation of electropherograms, demonstrating once more that the lack of a solid devised experimental approach is the main cause of error in mtDNA testing.


Subject(s)
Artifacts , Clinical Laboratory Techniques/standards , DNA Fingerprinting/standards , DNA, Mitochondrial/genetics , DNA/isolation & purification , Blood Stains , Computer Simulation , DNA/analysis , DNA/genetics , DNA, Mitochondrial/blood , DNA, Mitochondrial/chemistry , Data Interpretation, Statistical , Databases, Factual , Female , Forensic Medicine , Genetic Markers , Hair/chemistry , Haplotypes , Humans , Phylogeny , Polymerase Chain Reaction , Polymorphism, Genetic , Pregnancy , Quality Control , Reference Standards , Saliva/chemistry
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