ABSTRACT
The behavioral and biochemical studies showed that daily oral administration of sanumgerman (340 mg/kg, i.e. 1/5 LD50) for 3 days exerted a depressive influence on the central nervous system of the mouse. The mechanism of this action could be related to an inhibition of catecholaminergic and of serotoninergic central system activities.
Subject(s)
Antineoplastic Agents/pharmacology , Central Nervous System/drug effects , Germanium/pharmacology , Organometallic Compounds/pharmacology , Administration, Oral , Animals , Antineoplastic Agents/administration & dosage , Antineoplastic Agents/toxicity , Behavior, Animal/drug effects , Germanium/administration & dosage , Germanium/toxicity , Male , Mice , Organometallic Compounds/toxicityABSTRACT
In the behavioral and biochemical investigations performed on Albino Swiss mice it was found that sanumgerman possessed inhibitory properties on the CNS. The mechanism of this action seems to be connected with depression of central catecholaminergic and a stimulation of central serotoninergic neurons.
Subject(s)
Antineoplastic Agents/pharmacology , Germanium/pharmacology , Organometallic Compounds , Animals , Anticonvulsants , Antineoplastic Agents/toxicity , Body Temperature/drug effects , Brain Chemistry/drug effects , Catalepsy/chemically induced , Drug Interactions , Exploratory Behavior/drug effects , Germanium/toxicity , Lethal Dose 50 , Male , Mice , Motor Activity/drug effects , Nervous System Diseases/chemically induced , Reaction Time/drug effectsABSTRACT
The rate and mechanism of incorporation of polyene phosphatidylcholine (PPC) and lysophosphatidylcholine into rat brain have been studied with double labelled substrates administered intravenously. It was found that both substances could be taken up into the brain with transfer velocities of the order: lysophosphatidylcholine much greater than phosphatidylcholine greater than glycerophosphocholine. Similarities between the 3H/14C ratios of the substrates and the brain phosphatidylcholine, together with retention of the label in the original acyl moieties, as demonstrated by phospholipase A2 hydrolysis, gave conclusive proof that both PPC and lysophosphatidylcholine are taken up intact into the brain.
Subject(s)
Brain Chemistry , Lysophosphatidylcholines/metabolism , Animals , Brain/enzymology , Glycerylphosphorylcholine/metabolism , Kinetics , Phosphatidylcholines/metabolism , Phospholipases A/metabolism , Phospholipases A2 , Rats , Rats, Inbred StrainsSubject(s)
Phospholipids , Prostaglandins E, Synthetic/pharmacology , Prostaglandins, Synthetic/chemical synthesis , Animals , Blood Pressure/drug effects , Cats , Dose-Response Relationship, Drug , Guinea Pigs , Hydrolysis , In Vitro Techniques , Muscle Contraction/drug effects , Phosphatidylethanolamines/pharmacology , Phospholipids/chemical synthesis , Prostaglandins E/pharmacology , Prostaglandins E, Synthetic/metabolism , RatsABSTRACT
Lecithin:cholesterol acyltransferase (LCAT) has been partially purified by the combined method of ultracentrifugation and dextranblue-2000 4 B affinity chromatography. The enzyme was incubated with liposomes consisting of phosphatidylcholine-cholesterol in a molar ratio of 10/1. Chemically synthesized phosphatidylcholine substrates with labeled fatty acids in 1-and 2-position were chosen to evaluate the degree of transesterification. It was found that the fatty acid in the 1-position of phosphatidylcholine significantly influences cholesteryl ester formation, both by its direct involvement in the LCAT reaction and its contribution to the physico-chemical properties of phosphatidylcholine.
Subject(s)
Phosphatidylcholine-Sterol O-Acyltransferase , Phosphatidylcholines , Phosphatidylcholines/chemical synthesis , Chemical Phenomena , Chemistry , Cholesterol Esters/analysis , Fatty Acids/analysis , Humans , Molecular Conformation , Phosphatidylcholine-Sterol O-Acyltransferase/analysis , Phosphatidylcholine-Sterol O-Acyltransferase/isolation & purification , Phosphatidylcholines/analysisABSTRACT
The pharmacokinetics of orally applied "essential" phospholipids (EPL) (i.e. polyenyl phosphatidyl choline) was investigated in conscious and in anesthetized rats with the aid of radioactively labelled dilinolyl phosphatidylcholines (3H and 14C.). Absorption, distribution and metabolism of EPL were studied using autoradiography and by measuring the distribution of the abosrbed radioactivity in lymph, blood and internal organs, and by determining excretion and expiration.
