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4.
Biochimie ; 59(8-9): 687-92, 1977.
Article in French | MEDLINE | ID: mdl-563739

ABSTRACT

The treatment with ethylenediamine of Torulopsis candida yeast cell walls has permitted to isolate a mannoprotein. It has been possible to collect this glycoprotein in a pure state after separation of the different hydrosoluble products by gel filtrations and paper electrophoresis. This compound contains 62 per cent of mannose, 3 per cent of N-acetylglucosamine and 31 per cent of amino acids. The mannose units are attached by 1 leads to 2 linkage, short oligosaccharides are bound to serine of the peptide chain by O-glycosidic linkages while polysaccharides are attached to asparagine of this peptide chain by a N glycosidic linkages probably through the intermediate of chitobiose.


Subject(s)
Candida/analysis , Cell Wall/analysis , Glycoproteins , Amino Acids/analysis , Carbohydrates/analysis , Glycoproteins/isolation & purification , Mannose/analysis , Molecular Conformation
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