ABSTRACT
Because of a possible relationship between microenvironmental disturbances and meiotic abnormalities and of a straight relationship between lower-quality semen in patient carrying a varicocele and first meiotic non-disjunction, bilateral bipolar testicular biopsies are realized according the thermic differential gradient described in varicocele. Systematic meiotic studies of multiple testicular biopsies from 65 azoospermic men with bilateral varicocele were done in a multi-centric study on microsurgical correction of bilateral varicocele with microthermic intra-operative evaluation using minimally invasive thermal microsensors (Betatherm 10K3MCD2). In the present study abnormal temperature raising, histomorphometric abnormalities (spermatocyte arrest) and meiotic abnormalities (class IIC) are strongly correlated. In the ten patients submitted to another testicular biopsy procedure six months after surgery for TESE, normal thermal differential is registered and no meiotic abnormalities recurrences are found.
Subject(s)
Meiosis , Oligospermia/pathology , Varicocele/surgery , Biopsy , Body Temperature , Cell Nucleus/ultrastructure , Chromosome Aberrations , Humans , Infertility, Male/etiology , Male , Microelectrodes/statistics & numerical data , Microsurgery , Oligospermia/etiology , Pachytene Stage , Testis/blood supply , Testis/pathology , Thermometers/statistics & numerical data , Varicocele/complicationsABSTRACT
Because of the fall in population in Europe, the evaluation of causes of male infertility is becoming more and more important. Testicular biopsy is a useful method of examination in the area of assisted reproduction. Testicular biopsies of 85 infertile males were evaluated histologically. According to the sperm count, 31 patients had azoospermia, 22 oligo-astheno-teratozoospermia (OATS), 29 oligozoospermia, and 3 astheno-teratozoospermia. The biopsies were carried out by atraumatic microsurgery. Samples were taken from the upper inner and lower outer quadrant of both sides. Bouin-fixed, paraffin-embedded blocks were cut and HE slides examined. Samples taken from azoospermic patients showed atrophy, maturation arrest, normal histology or Sertoli cell-only syndrome. Oligozoospermic patients presented maturation arrest, hypospermatogenesis or normal histology. In the OATS group maturation arrest hypospermatogenesis and atrophy were found. The spermatogram groups showed significant correlation with the homogeneity of the spermatogenesis. The sperm count alone is not sufficient in assessing spermatogenesis. Four samples give more adequate information than just one, because spermatogenesis is not homogeneous in the testis. After evaluating diagnostic biopsies, tissue for TESE can be taken from the most appropriate location in the testis.
Subject(s)
Oligospermia/pathology , Sperm Injections, Intracytoplasmic/methods , Spermatozoa/pathology , Testis/pathology , Adult , Atrophy/pathology , Biopsy , Humans , Male , Middle Aged , Oligospermia/etiology , Sertoli Cells/pathology , Sperm Count , Spermatogenesis/physiologyABSTRACT
Authors have performed testis biopsy on 21 male patients according to the predesigned protocol. The biopsy has been carried out from scrotal excision, with the exposure of both testis with microsurgical method. The results have been evaluated according to the spermogram groups. Correlation of FSH values and histological appearance of both testis was analyzed. Their method and experiences have been evaluated the effectiveness of assisted reproduction.
