ABSTRACT
INTRODUCTION: The aim of our study was to evaluate the concordance between the two antifungal susceptibility testing of Candida sp. isolates using agar diffusion method: Neo-Sensitabs(®) tablets and Bio-Rad(®) disks. MATERIALS AND METHODS: This is a prospective study conducted in the Laboratory of Parasitology and Mycology of the Mohammed V military teaching hospital from February to August 2012. Upon receiving blood cultures and peripheral sites samples, the identification of Candida isolates performed using routine phenotypic standard tests and the realization of the antifungal susceptibility was carried out on Neo-sensitabs(®) tablets and Bio-Rad(®) disks. RESULTS: A total of 38 Candida strains were isolated: 15 C. albicans (39%), 13 C. glabrata (34%), 5 C. tropicalis (13%), 4 C. krusei (11%) and 1 C. dubliniensis (3%). There were no significant difference (P>0.05) in susceptibility rate between both methods for all antifungal agents tested except for 5-fluorocytosine. The concordance percentage between two methods was 100% for amphotericin B, 97.4% for fluconazole, 94.7% for voriconazole and 73% for 5-fluorocytosine. CONCLUSION: Both methods are easy to perform, rapid and cost effective. Our results showed the best agreement between the two methods for testing the susceptibility of Candida isolates to amphotericin B, fluconazole and voriconazole while for the 5-fluorocytosine, the concordance rate was low.
Subject(s)
Antifungal Agents/pharmacology , Candida/drug effects , Immunodiffusion/methods , Microbial Sensitivity Tests/methods , Amphotericin B/pharmacology , Fluconazole/pharmacology , Flucytosine/pharmacology , Humans , Prospective Studies , Tablets , Voriconazole/pharmacologyABSTRACT
Intestinal parasitic infections are among the most widespread of human infections in developing countries, and children are the most vulnerable. The aim of this study was to determine the prevalence of protozoa and intestinal helminthes, as well as the risk factors of intestinal parasites in schoolchildren in Salé city, Morocco. This is a study of incidence and prevalence conducted prospectively over a period of five months in schoolchildren in Salé city. The collection of stool was performed over three days (j1, j3, j5). In addition, adhesive cellophane tape slide evaluation was performed on day 7 for all students included in this work. During the study period, we included 123 students. Seventy-six children (61.7%) were infected by intestinal parasites. The age group 12-14 years is by far the most affected. Protozoa were found in 57.7% (N = 71) of children examined. Amoebae family parasites were predominant. Helminths were present in 26% (N = 32) of the schoolchildren. Forty-five (36.6%) children were poly-parasitized. This work shows that the prevalence of intestinal parasitism is quite high among primary schoolchildren in Salé city. Several parasite species are found. This finding is explained by unhealthy living conditions and poor hygiene, predisposing to endemicity and perpetuation of the transmission. The impact on health is not negligible especially when compounded by malnutrition. The best way to fight this scourge is prevention and awareness.
Subject(s)
Intestinal Diseases, Parasitic/epidemiology , Schools/statistics & numerical data , Adolescent , Child , Female , Humans , Intestinal Diseases, Parasitic/etiology , Intestinal Diseases, Parasitic/parasitology , Male , Morocco/epidemiology , Prevalence , Risk Factors , Urban Population/statistics & numerical dataABSTRACT
INTRODUCTION: Mucormycosis is the third among deep fungal opportunistic infections after candidiasis and aspergillosis. It is a rare fungal infection in children, but often fatal, which occurs in immunocompromised patients. It is caused by fungi belonging to the order of mucorales. It causes extensive damage and decaying soft parts. The authors report the case of a sinonasal mucormycosis with fatal outcome in a child suffering from hemophagocytic syndrome. REPORT: PL, aged 23 months, resulting from non-consanguineous parents, hospitalized for management of hemophagocytic syndrome lasting for 2 months suspected on clinical and biological data. This diagnosis was confirmed on histology. The etiological diagnosis was negative. A broad-spectrum antibiotics and corticosteroids was introduced. A month later, the patient developed necrotic lesions in the nose and facing the right maxillary sinus. CT scan of facial mass objectived ethmoïdomaxillary bilateral sinusitis. The mycological examination of a nasal swab showed the presence of non-compartmentalized hyphae, culture on Sabouraud chloramphenicol medium without actidione at 37°C isolated Absidia corymbifera. Treatment with amphotericin B was initiated but not tolerated. The negative trend was rapidly leading to death. CONCLUSION: Rhinocerebral mucormycosis is a rare fungal infection in children, we must know how to keep it in mind. The mycological examination and/or histology of a local levy allows rapid diagnosis. Treatment should be initiated urgently to improve the prognosis.
ABSTRACT
We report a case of severe keratitis due to Fusarium solani in a young man in the Sahara in Morocco where the climate is arid. This patient reported had a grain of sand in his right eye for a week after a sandstorm. On admission he had a corneal abscess. Despite rapid diagnosis and initiation of treatment with available antifungal drugs: amphotericin B and natamycin eye drops, the prognosis worsened and led to the enucleation of the right eye. Faced with a suspected eye infection after a microtrauma caused by grains of sand carried by a sandstorm, it is important to take biological samples to search for fungal infections among other. It is also important to have new triazole antifungal drugs available to treat ocular mycosis rapidly and effectively.
