Construction of a recombinant lentivirus-mediated shRNA expression vector targeting the human PSMD10 gene and validation of RNAi efficiency in RPMI­8226 multiple myeloma cells.

Multiple myeloma (MM) is one of the most common malignant blood cancers. Previous studies have reported that proteasome 26S subunit non-ATPase 10 (PSMD10) is an oncoprotein with complex roles in hepatocellular carcinoma and other malignant tumors. Notably, research on the relationship between PSMD10 and tumorigenesis of MM has rarely been reported. The present study was designed to explore the possibility of PSMD10 as a therapeutic target in the treatment of MM, and the use of RNA interference (RNAi) to determine the function PSMD10. A recombinant lentivirus-mediated short hairpin RNA (shRNA) targeting human PSMD10 mRNA was constructed and used to decrease endogenous PSMD10 expression in the MM RPMI-8226 cell line in vitro. Expression of the PSMD10-targeting shRNA in RPMI-8226 cells transduced with the recombinant vector could be tracked by observing the expression of green fluorescent protein after infection. A transient transgenic RPMI-8226 cell line was generated by transducing cells with the packaged viral particles. Western blot analysis indicated that the protein levels of PSMD10 in the PSMD10-shRNA MM cells were significantly lower than those in the cells transduced with the negative control shRNA. Notably, RT-qPCR analysis did not reveal a marked change in the PSMD10 mRNA level; thus, the knockdown effect of the PSMD10-shRNA may occur during translation. Furthermore, apoptosis of MM cells was increased by silencing PSMD10 expression. Overall, the results demonstrated that the lentivirus-mediated shRNA vector-based RNAi expression system is an efficient method to silence PSMD10 gene expression in the MM RPMI-8226 cell line. It may provide a basis to study the role of PSMD10 in tumor cells, and may be a reliable gene therapy strategy in the clinic.

Diagnostic value of using 18F-FDG PET and PET/CT in immunocompetent patients with primary central nervous system lymphoma: A systematic review and meta-analysis.

BACKGROUND: 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography (PET) and PET/CT have become two of the most powerful tools for malignant lymphoma exploration, but their diagnostic role in primary central nervous system lymphoma (PCNSL) is still disputed. The purpose of our study is to identify the usefulness of 18F-FDG PET and PET/CT for detecting PCNSL. RESULTS: A total of 129 patients, obtained from eight eligible studies, were included for this systematic review and meta-analysis. The performance of 18F-FDG PET and PET/CT for diagnosing PCNSL were as follows: the pooled sensitivity was 0.88 (95% CI: 0.80-0.94), specificity was 0.86 (95% CI: 0.73-0.94), positive likelihood ratio (PLR) was 3.99 (95% CI: 2.31-6.90), negative likelihood ratio (NLR) was 0.11 (95% CI: 0.04-0.32), and diagnostic odds ratio (DOR) was 33.40 (95% CI: 10.40-107.3). In addition, the area under the curve (AUC) and Q index were 0.9192 and 0.8525, respectively. MATERIALS AND METHODS: PubMed/MEDLINE, Embase and Cochrane Library were systematically searched for potential publications (last updated on July 16th, 2016). Reference lists of included articles were also checked. Original articles that reported data on patients who were suspected of having PCNSL were considered suitable for inclusion. The sensitivities and specificities of 18F-FDG PET and PET/CT in each study were evaluated. The Stata software and Meta-Disc software were employed in the process of data analysis. CONCLUSIONS: 18F-FDG PET and PET/CT showed considerable accuracy in identifying PCNSL in immunocompetent patients and could be a valuable radiological diagnostic tool for PCNSL.

Primary testicular lymphoma with subcutaneous masses as the sole manifestation of the first relapse and central nervous system lymphoma as the second relapse: A case report and literature review.

Primary testicular lymphoma (PTL) accounts for ~1% of all non-Hodgkin's lymphomas and has a marked tendency for systemic relapse. The current study presents a unique case of testicular diffuse large B-cell lymphoma of non-germinal center B-cell subtype, with subcutaneous masses as the sole manifestation of the first relapse and central nervous system lymphoma as the second relapse. Subcutaneous relapse and subsequent brain relapse are extremely rare signs of PTL dissemination. The patient received methotrexate-based combined chemotherapy and achieved a partial response. This case presents a rare pattern of treatment failure in this malignant clinical entity.

Predictors of return to work and duration of absence following work-related hand injury.

The aims of the study were to explore the situation and the potential determinants of return-to-work (RTW) and the absence duration following work-related hand injury, and to provide evidence for the future intervention strategy of improving RTW. A prospective cohort of workers with work-related hand injury from three selected hospitals in East China was followed up on the outcomes of RTW up to 8 months after discharge. Demographic and clinical data were collected during admission; economic factors, psychological factors and RTW outcomes were, respectively, investigated using a structured questionnaire via phone call after discharge from the hospitals in 0.5 month, 2 months, 4 months and 8 months. Univariate analysis and Cox regression model were used to examine the associations between potential determinants and outcomes of the RTW. Out of the 246 cases, 192 (78.1%) eventually returned to work with the median duration of the absence of 44.0 days during the 8-month follow-up. Factors from demographic, clinical, economic and psychological domains affected RTW in the univariate analyses. Receiving timely treatment at outpatient clinics, less serious injury, no tendon trauma and no skin loss were found to be significantly beneficial to RTW, while workers with the decreased monthly salary during absence and lower pre-injury salary were likely to take longer sick leave. Most of the workers successfully achieved RTW after work-related hand injury. Proper clinical treatment and rehabilitation, as well as economic and social support seem to have played vital roles in prompting RTW that should be prioritised for the intervention strategy.

Hydrogen storage properties of Li-Mg-N-H systems with different ratios of LiH/Mg(NH2)2.

In this work, the hydrogen desorption and structural properties of the Li-Mg-N-H systems with different LiH/Mg(NH2)2 ratios are systemically investigated. The results indicate that the system with the LiH/Mg(NH2)2 ratio of 6/3 transforms into Li2NH and MgNH, and then, the mixture forms an unknown phase by a solid-solid reaction, which presumably is the ternary imide Li2Mg(NH)2; the system with the LiH/Mg(NH2)2 ratio of 8/3 transforms into 4Li2NH and Mg3N2 after releasing H2 at T < 400 degrees C; the system with the LiH/Mg(NH2)2 ratio of 12/3 transforms into 4Li3N and Mg3N2 after releasing H2 at T > 400 degrees C, where the LiMgN phase is formed by the reaction between Li3N and Mg3N2. The characteristics of the phase transformations and the thermal gas desorption behaviors in these Li-Mg-N-H systems could be reasonably explained by the ammonia mediated reaction model, irrespective of the difference in the LiH/Mg(NH2)2 ratios.

Mechanism of hydrogenation reaction in the Li-Mg-N-H system.

The Li-Mg-N-H system composed of 3 Mg(NH2)2 and 8 LiH reversibly desorbs/absorbs approximately 7 wt % of H2 at 120-200 degrees C and transforms into 4 Li2NH and Mg3N2 after dehydrogenation. In this work, the mechanism of the hydrogenation reaction from 4 Li2NH and Mg3N2 to 8 LiH and 3 Mg(NH2)2 was investigated in detail. Experimental results indicate that 4 Li2NH is first hydrogenated into 4 LiH and 4 LiNH2. At the next step, 4 LiNH2 decomposes into 2 Li2NH and 2 NH3, and the emitted 2 NH3 reacts with (1/2) Mg3N2 and produces the (3/2) Mg(NH2)2 phase, while the produced 2 Li2NH is hydrogenated into 2 LiH and 2 LiNH2 again. Such successive steps continue until all 4 Li2NH and Mg3N2 completely transform into 8 LiH and 3 Mg(NH2)2 by hydrogenation.