Influence of clinical zirconia surface treatments on microscopic characteristics and adhesion-proliferation behavior of human gingival fibroblasts.

Zirconia is favored in dental implant applications due to its biocompatibility, mechanical properties, and esthetic appeal, particularly in its interaction with soft oral tissues such as the gingiva. To optimize zirconia for clinical use, surface treatments like sanding and polishing are essential. The aim of this study was to investigate the effects of clinical surface treatments on the microscopic characteristics of zirconia and the adhesion and proliferation of human gingival fibroblasts (HGFs). Scanning electron microscopy (SEM) and fluorescence microscopy were utilized to examine the microscopic morphology and roughness resulting from various clinical surface treatment procedures on zirconia and to assess their impact on the microscopic appearance and behavior of HGFs. The results showed that the application of surface treatment procedures, particularly polishing treatments, resulted in the formation of a regular shallow groove morphology and a significant reduction in roughness in zirconia. This was accompanied by improved cell proliferation, cell adhesion, and the expression of integrin ß1 in HGFs. The results suggest that smoother zirconia surfaces promote better cell-material interactions, potentially improving the clinical success of dental implants. This research contributes to our understanding of the optimal surface roughness for soft tissue adhesion and the effect of different micro-morphologies on HGF attachment.

Effect of calcium hydrothermal treatment of zirconia abutments on human gingival fibroblasts.

Zirconia materials have been increasingly used in implant rehabilitation due to their excellent physical and esthetic properties. Stable peri-implant epithelial tissue adhesion to the transmucosal implant abutment may significantly enhance the efficacy of implant long-term stability. However, it is difficult to form stable chemical or biological bindings with peri-implant epithelial tissue due to the strong biological inertia of zirconia materials. In the present study, we investigated whether calcium hydrothermal treatment of zirconia promotes sealing of peri-implant epithelial tissue. In vitro experiments were performed to analyze the effects of calcium hydrothermal treatment on zirconia surface morphology and composition by scanning electron microscopy and energy dispersive spectrometry. Immunofluorescence staining of adherent proteins, namely, F-actin and integrin ß1, in human gingival fibroblast line (HGF-l) cells was performed. In the calcium hydrothermal treatment group, there was higher expression of these adherent proteins and increased HGF-l cell proliferation. An in vivo study was conducted by extracting the maxillary right first molars of rats and replacing them with mini-zirconia abutment implants. The calcium hydrothermal treatment group showed better attachment at the zirconia abutment surface, which inhibited horseradish peroxidase penetration at 2 weeks post-implantation. These results demonstrated that calcium hydrothermal treatment of zirconia improves the seal between the implant abutment and surrounding epithelial tissues, potentially increasing the long-term stability of the implant.