ABSTRACT
OBJECTIVE: The aim of this study was to screen the differential genes related to ferroptosis in osteoporosis patients. MATERIALS AND METHODS: GEO2R was used to screen the differential genes related to ferroptosis in osteoporosis patients by searching the relevant chips in the GEO database, and Spearman's correlation analysis was used to describe the correlation between quantitative variables without normal distribution. p-values lower than 0.05 were considered statistically significant. Another group of osteoporosis patients was selected in the GEO database to verify the significantly differentially expressed genes. RESULTS: The results showed that 10 samples in chip GSE35956 were identified as research objects, and a total of 5 ferroptosis differential genes were screened out: ATP5MC3, CDKN1A, MT1G, NCOA4, SLC1A5, of which 3 up-regulated genes (CDKN1A, MT1G, SLC1A5), 2 down-regulated genes (ATP5MC3, NCOA4). The above differential genes were placed in 19 samples of chip GSE35959 for verification, and the same expression trend was obtained, but only the MT1G difference was statistically significant. CONCLUSIONS: The gene correlation test found that MT1G and ATP5MC3 had a strong negative correlation.
Subject(s)
Ferroptosis , Osteoporosis , Humans , Ferroptosis/genetics , Databases, Factual , Gene Expression , Osteoporosis/genetics , Reference Values , Minor Histocompatibility Antigens , Amino Acid Transport System ASCABSTRACT
This paper reports the determination of promethazine hydrochloride with Nafion coated glassy carbon electrode by anodic stripping voltammetry. There is a linear relationship between the concentration and peak height in the range of 4 x 10(-8)-5 x 10(-5) mol/L and 8 x 10(-5)-1 x 10(-3) mol/L for promethazine hydrochloride. The raw material and tablets of promethazine hydrochloride have been analysed and good results were obtained.
Subject(s)
Promethazine/analysis , Electrodes , Fluorocarbon PolymersABSTRACT
This paper discusses the reasons why the stability of procaine coated wire electrode is bad. A coated wire electrode was made by coating polyvinyl alcohol (containing procaine hydrochloride) on Ag-AgCl wire followed by covering with PVC membrane. This electrode show to have fine response performance, the stability is also improved greatly.
Subject(s)
Electrodes , Procaine/analysisABSTRACT
Using platinum plate coated with PVC membrane as indicator electrode, amitriptyline hydrochloride was titrated with sodium tetraphenylborate by zero-current oscillo-potentiometry titration and zero-current oscillo-bipotentiometric titration. Both methods are precise, audio-visual, simple and convenient and can be used in the determination of other medicines.
