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1.
Metabolites ; 12(10)2022 Oct 11.
Article in English | MEDLINE | ID: mdl-36295863

ABSTRACT

Vitamin B micronutrients are essential regulators of one carbon metabolism that ensures human health. Vitamin B9, or folate, lies at the heart of the folate cycle and converges with the methionine cycle to complete the one carbon pathway. Additionally, vitamin B6 contributes by orchestrating the flux of one carbon cycling. Dysregulation of vitamin B contributes to altered biochemical signaling that manifests in a spectrum of human diseases. This review presents an analysis of the past, present, and future work, highlighting the interplay between folate and vitamin B6 in one carbon metabolism. Emerging insights include advances in metabolomic-based mass spectrometry and the use of live-cell metabolic labeling. Cancer is used as a focal point to dissect vitamin crosstalk and highlight new insights into the roles of folate and vitamin B6 in metabolic control. This collection of vitamin-based research detailing the trends of one carbon metabolism in human disease exemplifies how the future of personalized medicine could unfold using this new base of knowledge and ultimately provide next-generation therapeutics.

2.
Nat Biotechnol ; 28(12): 1291-4, 2010 Dec.
Article in English | MEDLINE | ID: mdl-21113166

ABSTRACT

The construction of synthetic biological systems involving millions of nucleotides is limited by the lack of high-quality synthetic DNA. Consequently, the field requires advances in the accuracy and scale of chemical DNA synthesis and in the processing of longer DNA assembled from short fragments. Here we describe a highly parallel and miniaturized method, called megacloning, for obtaining high-quality DNA by using next-generation sequencing (NGS) technology as a preparative tool. We demonstrate our method by processing both chemically synthesized and microarray-derived DNA oligonucleotides with a robotic system for imaging and picking beads directly off of a high-throughput pyrosequencing platform. The method can reduce error rates by a factor of 500 compared to the starting oligonucleotide pool generated by microarray. We use DNA obtained by megacloning to assemble synthetic genes. In principle, millions of DNA fragments can be sequenced, characterized and sorted in a single megacloner run, enabling constructive biology up to the megabase scale.


Subject(s)
DNA/chemical synthesis , Genes, Synthetic , Oligonucleotide Array Sequence Analysis/methods , Polymerase Chain Reaction/methods , DNA/chemistry , Humans , Oligonucleotides/chemical synthesis , Oligonucleotides/chemistry , Robotics
3.
Electrophoresis ; 25(15): 2461-8, 2004 Aug.
Article in English | MEDLINE | ID: mdl-15300763

ABSTRACT

Proteome analysis represents significant challenges to the existing sample preparation techniques. Traditional methods, such as two-dimensional electrophoresis, typically separate high-molecular-weight proteins while discarding low-molecular-weight species. This approach is well justified considering the complexity of any proteome. However, it is desirable to extract the maximum amount of information from each sample to investigate the entire range of biomolecules. We have demonstrated that ultrafiltration not only improves two-dimensional electrophoresis (2-DE) resolution of the protein fraction but also yields the low-molecular-weight fraction amenable for further analysis by high-resolution mass spectrometry. This approach was successfully adapted to the variety of biological samples including cell and tissue lysates and serum. Therefore, ultrafiltration offers an alternative sample preparation technique that enables more thorough analysis of a proteome.


Subject(s)
Electrophoresis, Gel, Two-Dimensional/methods , Liver/metabolism , Proteome , Ultrafiltration/methods , Animals , Breast Neoplasms/metabolism , Cattle , Humans , Molecular Weight , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods , Tumor Cells, Cultured
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