[A FITZ-HUGH-CURTIS SYNDROME AS A PREMISE OF THE INFLAMMATORY DISEASES OF A HEPATOPANCREATOBILIARY ZONE ORGANS].

A content of a biliary ways microflora and its correlation with clinical form and severity of inflammatory-destructive processes in hepatopancreatobiliary zone were studied up. The investigation objective was the optimization of a treatment--diagnostic tactics for the complications and remote recurrences rate reduction in a complicated biliary calculous disease. There was established, that a standard bacteriological investigation do not give a possibility to estimate some causes of a biliary ducts affection, a chlamidial infection in particular. So on, for the individual antibacterial therapy choosing a more detailed and modern examination of patients is needed.

[THE FORMATION OF BIOFILM IN OPPORTUNISTIC MICROORGANISMS IN BLOOD PLASMA DEPENDING ON CONTENT OF IRON].

The article considers results of analysis offormation of biofilm of priority opportunistic pathogens in blood plasma and LB-broth. As compared with LB-broth, bloodplasma stimulates formation of biofilm of microorganisms in the following sequence: Staphylococcus aureus > Pseudomonas aeruginosa > Escherichia coli. The application oftechnique of infra-redspectroscopy of bio-films established that blood plasma promotes formation of external exopolysaccharides of S.aureus. The cultivation of bio-films in plasma depending on content of iron demonstrated that the analyzed strains of S. aureus, P. aeruginosa, E. coli form bio-films in a better way in plasma with normal content of iron and iron-deficient and iron-loaded plasma decreases their activity of formation of biofilm.

[The increasing and expression of virulence factors of opportunistic microorganisms in blood serum under various alternatives of iron homeostasis].

Staphylococcus aureus and Pseudomonas aeruginosa are foregroundpathogens of bacteriemia and sepsis. They produce large spectrum of such factors of pathogenicity permitting them to proliferate and survive in bloodstream as hydrolytic enzymes, adenosine diphosphate-ribosylarginine toxins, plasmocoagulase, etc. The occurrence of alteration of growth and expression of virulence factors of S. aureus and P. aeruginosa at fermentation in LB-broth depending on iron concentration was demonstrated previously. The conditions in vivo significantly differ the laboratory conditions. The activity of growth and expression of pathogenicity factors of S. aureus and P. aeruginosa at fermentation in blood serum of donors with different alternative of iron homeostasis was analyzed. The study established expression of genes of hemolytic phospholipase C (plcH), alginate (algD), exotoxin A (exoA) for P. aeruginosa and genes of protein A (spA), virulence global regulator (RNAIII) for S. aureus. The iron-deficient serum and serum with normal iron homeostasis decreased activity of growth of S. aureus and P. aeruginosa. The growth rate and expression level of all analyzed genes turned out higher at fermentation of S. aureus and P. aeruginosa in serums containing excess of iron (more than 30 mkM). The fermentation of P. aeruginosa in iron-deficient serum resulted in decreasing of expression level of genes plcH, algD, exoA. The expression of RNA III and spaA S.aureus in iron-deficient serum increased towards normal content of iron in blood. The example of S. aureus and P. aeruginosa demonstrated that expression of many virulence factors of opportunistic microorganisms depends on iron homeostasis of host organism. The survival and proliferation of microorganisms in blood depend on both immune status of host organism and biological characteristics of pathogen.

[The etiologic structure and biologic characteristics of agents of infections of bloodstream.]

As before, the infections of blood flow remain actual because of absence of universal approaches to their diagnostic and high lethality. Taking into account the fact that most frequently the sepsisogenic strains are selected in blood, studying of biological characteristics of hemocultures in comparison with strains isolated from other biotopes is very important for understanding the mechanisms of survival of pathogens in blood and prognosis development of septic complications. The article presents the results of microbiological analysis of blood carried out in multi-field clinic of Khanti-Mansiisk during 2007-2015. The following biological characteristics of S.aureus, E.coli, P.aeruginosa, C.albicans: hemolytic, catalase, anti-lysozyme, anti-complementary, biofilm forming, activity of hemocultures and strains isolated from other biotopes. The detection of hemolytic, catalase, anti-lysozyme, anti-complementary activities was implemented using photo-metric technique by capacity to lyse/inactivate corresponding substrate. The biofilm forming activity was evaluated according alteration of contact angle of moistening of surface of biofilm. The priority pathogens of infections of blood stream are coagulase-positive staphylococci - 38.3% (S.aureus), coagulase-negative staphylococci (S.epidermidis, S.hominis etc.), enterobacteria - 12.3% (E.coli, K.pneumonia), Pseudomonas aeruginosa - 4.1%, among fungi - C.albicans (2.5%). It is demonstrated that, in comparison with strains isolated from other biotops, hemocultures have statistically reliable higher values of catalase, anti-lysozyme, anti-complementary, biofilm forming activities. The conclusion is made concerning significance of these biological characteristics of pathogens for their survival in blood. It is proposed to use these indices as markers of sepsisogenic strains in prognosis of generalization of inflectional process.

[Immediate and long-term results of surgical treatment of benign breast disease].

Sector resection of the breast is the standard surgical treatment of benign breast disease, but often it has unsatisfactory outcomes. The aim of the study was a comparative analysis of the effectiveness of surgical treatment of patients with benign breast diseases after sector resection and after modification of the procedure. Classical sector resection was performed on 45 women, 106 - underwent operations with using of plastic surgery elements. The effectiveness of treatment was defined by the presence of complications in the early postoperative period; the quality of life was studied using questionnaires--The Short Form Medical Study (SF-36) and The Breast Evaluation Questionnaire (BEQ)--before surgery and after 3 and 12 months after surgery. After sector resection was 44% of the complications in the early postoperative period, after the developed techniques - 17%. 3 months after the operation quality of life and aesthetic satisfaction of breast in patients of both groups decreased. 1 year after sector resection the rates of satisfaction of breast aesthetic appearance were significantly lower than the preoperative level. In patients after modified operations at 1 year was observed a significant improvement in well-being and satisfaction of aesthetic appearance of breast. The method of sector resection has a greater number of complications; it leads to deterioration of the aesthetic appearance of the breast in 1 year after surgery. Surgery techniques with elements of mammaplasty have fewer complications and improve quality of life after surgery.

[Polymorphism of gene of ІL-8 (А-251T) in patients with acute pancreatitis].

The research aims to investigate the IL-8 А-251Т (rs 4073) gene polymorphism in patients with acute pancreatitis. The frequency of allelic variants of cytokine gene in 100 patients was determined. Genotype А/Т was found in 47.0% of cases (47 patients) of acute pancreatitis, T/T alleles - in 37% of cases (37 patients), A/A alleles in 16% of cases (16 patients). In 73.3% of non-operated patients with destructive forms of acute pancreatitis the reliable (р<0,05) increase of heterozygous carriers of genotype А/Т were detected. In patients with edematous form of acute pancreatitis frequency of А/Т was detected in 42.2% of cases, In control group frequency of А/Т was detected in 46% of cases, The possible association between genotype А/Т and the destruction of the pancreas was determined.

[Relation of hydrophobicity index of microbial cells to their biofilm-forming ability].

By examining cell adhesion to n-hexadecane the hydrophobicity index of S. aureus, E. coli and P. aeruginosa was measured. It has been established that the increase in the hydrophobicity of the microbial cells reduces their ability to form a biofilm. It is shown that the divalent metal ions are capable of increasing the hydrophobicity index of microbial cells and reducing biofilm formation. It is concluded that it is necessary to use materials with a negatively charged surface for biofilm preventive measures.

[The quantitative evaluation of capacity of opportunistic pathogenic microorganisms to form biofilms in experiment].

The article discusses the technique of quantitative evaluation of capacity of opportunistic pathogenic microorganisms to form biofilm by means of measuring the contact angle of moistening of its surface. The specific rates of biofilm formation by hospital strains of microorganisms are determined. It is demonstrated that P. aeruginosa form biofilm on surface of glass plate better than S. aureus and C. albicans do.

Iron-dependent synthesis of hemolysins by Staphylococcus aureus.

We studied the effect of Fe(2+) on hemolysin synthesis by Staphylococcus aureus. Hemolytic activity of staphylococci was shown to be iron-dependent. Addition of Fe(2+) to the nutrient medium induced the synthesis of α-hemolysin by staphylococci. Increasing the concentration of Fe(2+) was followed by an increase in hemolytic activity of staphylococci. Inducible synthesis of α-hemolysin probably serves as an important pathogenetic factor in the development of staphylococcal infection in patients with excessive iron accumulation in the body.

[The treatment process optimization in patients suffering from acute disorder of mesenteric blood circulation].

The results of treatment of 253 patients, suffering an acute disorder of mesenteric blood circulation, were analyzed; the treatment program, applied in 55 patients, was optimized. Timely diagnosis in the tissues the ischemia stage as well as during restoration of blood circulation in a. mesenterica superior, while its proximal occlusion, application of a "second-look" tactics have had secured beneficial results of treatment in patients, the lethality was 33%. Intraoperative application of redoxmetry for estimation of the intestine life capacity have had promoted prophylaxis of the sutures insufficiency in interintestinal anastomoses. Optimization of the treatment tactics (two-staged surgical treatment with retrograde jejunal intubation, the postponed formation of anastomosis) gave possibility to reduce lethality in patients, suffering arterial occlusion from 75 to 60% (P<0.05).

[The investigation of the composition of liquid radioactive waste].

In investigation the process of composition sediment of liquid unorganic radioactive waste, that are forming in cistern-selectors at PNPI RAS, it was discovered apart from great quantity of ions of different metals and radionuclides considerable maintenance of organic material (to 30% and more from volume of sediment) unknown origin. A supposition was made about its microbiological origin. Investigation shows, that the main microorganisms, setting this sediment, are the bacterious of Pseudomonas kind, capable of effectively bind in process of grow the radionuclide 90Sr, that confirms the potential posibility of using this microorganisms for bioremediation of liquid low radioactive wastes (LRW).

[Modern views on the efficacy of different antibacterial preparations in the treatment and prophylaxis of inflammatory processes in patients after transurethral local ultrasonic hyperthermia of the prostate]. / Sovremennyi vzgliad na éffektivnost' primeneniia antibakterial'nykh preparatov raznykh grupp v lechenii i metafilaktike vospalitel'nykh protsessov u patsientov posle transuretral'noi lokal'noi mikrovolnovoi gipertermii predstatel'noi zhelezy.

Extensive clinical investigation was accomplished to study the influence of three antibacterial preparations groups (tetracyclines, aminoglycosides and fluorochinolons) on the phagocytic-macrophages and the immune systems in patients, to whom transurethral local microwave hyperthermia (TLMH) of prostate was performed for its benign hyperplasia. The data obtained witness high efficacy of fluorochinolons group preparation (tavanic) toward bacteria of different nosologic groups, as well as to their atypical forms. Pronounced efficacy and simplicity of application of the preparation had allowed to recommend its wide use in urologic practice.