ABSTRACT
The effect of uncoupler 2,4-dinitrophenol on the oxidative processes intensity in liver biomembranes of different age and sex of rats during longitudinal experiment was studied. It was established that 2,4-dinitrophenol in the used concentration hadn't essential negative effect on the organism of females and their posterity. On the young, 3-3,5-month old males it was shown that long-term xenobiotic administration had been accompanied by intensification of the rate of oxygen consumption, decrease of the rate of reactive oxygen species formation in microsomal redox-chain, decline in lipid hydroperoxides and protein carbonils levels in blood serum and liver microsomes, and also by increase of their mean lifespan. The obtained results may testify the possibility of correction of oxidative processes intensity in tissues of mammals and their lifespan by means of modulation of membrane electron transport chains activity.
Subject(s)
2,4-Dinitrophenol/pharmacology , Aging/metabolism , Liver/drug effects , Oxidative Stress/drug effects , Uncoupling Agents/pharmacology , Aging/drug effects , Animals , Electron Transport/drug effects , Female , Liver/growth & development , Liver/metabolism , Liver Function Tests , Male , Maternal Exposure , Mitochondria, Liver/drug effects , Mitochondria, Liver/metabolism , Oxygen Consumption/drug effects , Pregnancy , Rats , Rats, Wistar , Time FactorsABSTRACT
The effect of xenobiotics on protein oxidative damage and lifespan of Drosophila melanogaster Meig., line Oregon-R, was studied. Addition of the uncoupler 2,4-dinitrophenol (DNP) to the nutritional mixture results in an induction of synthesis of heat shock proteins and increase of lifespan of insects, whereas sodium nitroprusside (SNP) has negative effect on flies viability connected, probably, with activation of processes of proteins oxidative damage. It is shown that DNP essentially corrects the SNP negative action on insects' survival rates and this "normalizing" action is revealed both at a level of sensitivity of flies to exogenic stresses and protein carbonils level and at a level of insects lifespan as a whole. It is supposed that DNP protects from SNP negative action on flies viability by reduction of intensity of free radicals production and/or induction of heat shock proteins synthesis. Consequence of that is reduction of oxidative proteins damage degree and increase of survival rate (life span) of flies.
