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1.
Cancer Cell ; 29(4): 587-601, 2016 Apr 11.
Article in English | MEDLINE | ID: mdl-27070705

ABSTRACT

The immune response influences the clinical course of colorectal cancer (CRC). Analyzing the invasive margin of human CRC liver metastases, we identified a mechanism of immune cell exploitation by tumor cells. While two distinct subsets of myeloid cells induce an influx of T cells into the invasive margin via CXCL9/CXCL10, CCL5 is produced by these T cells and stimulates pro-tumoral effects via CCR5. CCR5 blockade in patient-derived functional in vitro organotypic culture models showed a macrophage repolarization with anti-tumoral effects. These anti-tumoral effects were then confirmed in a phase I trial with a CCR5 antagonist in patients with liver metastases of advanced refractory CRC. Mitigation of tumor-promoting inflammation within the tumor tissue and objective tumor responses in CRC were observed.


Subject(s)
Adenocarcinoma/secondary , Chemokine CCL5/antagonists & inhibitors , Colorectal Neoplasms/immunology , Liver Neoplasms/secondary , Molecular Targeted Therapy , Neoplasm Proteins/antagonists & inhibitors , Receptors, CCR5/drug effects , Adenocarcinoma/drug therapy , Adenocarcinoma/immunology , Apoptosis/drug effects , Chemokine CCL5/biosynthesis , Chemokine CCL5/metabolism , Chemokines/physiology , Chemotaxis , Clinical Trials, Phase I as Topic , Clodronic Acid/pharmacology , Cyclohexanes/pharmacology , Cyclohexanes/therapeutic use , Humans , Interferon-alpha/metabolism , Liver Neoplasms/drug therapy , Liver Neoplasms/immunology , Liver Neoplasms/metabolism , Lung Neoplasms/drug therapy , Lung Neoplasms/secondary , Lymphocytes, Tumor-Infiltrating/immunology , Lymphocytes, Tumor-Infiltrating/metabolism , Macrophages/drug effects , Macrophages/metabolism , Maraviroc , NG-Nitroarginine Methyl Ester/pharmacology , Neoplasm Invasiveness , Neoplasm Proteins/physiology , Phenylurea Compounds/therapeutic use , Pilot Projects , Pyridines/therapeutic use , Receptors, CCR5/metabolism , STAT3 Transcription Factor/physiology , Survival Analysis , Triazoles/pharmacology , Triazoles/therapeutic use , Tumor Cells, Cultured , Tumor Microenvironment/drug effects
2.
Oncoimmunology ; 2(4): e24116, 2013 Apr 01.
Article in English | MEDLINE | ID: mdl-23734335

ABSTRACT

The immune system plays an important role in shaping the clinical course of colorectal cancer (CRC). However, it is still unclear how the immune infiltrates of primary CRC lesions and distant metastases by immune effector cells are related to each other. To address this issue, we quantified CD3+, CD8+ and granzyme B+ lymphocytes in primary CRC samples and corresponding liver metastases. This analysis showed that the prognostic predictions that can be drawn from the infiltration of immune cells in primary CRCs and their metastases are heterogeneous. To investigate whether such heterogeneity would also be observed within CRC hepatic metastases, the density of the immune infiltrate and cytokine production were assessed in opposite sides of the same metastatic lesion. In addition, tumor-infiltrating lymphocytes were assessed in sequential sections of the same metastatic lesion, with a spacing of 30 µm. In summary, consistent cell counts and cytokine levels were detected within the same lesion. The study of a case of synchronous metastases, however, suggested that different metastatic lesions within the same patient may be heterogeneous, perhaps indicating a major impact for local causes on tumor infiltration by immune cells. In summary, our study demonstrates a consistent degree of heterogeneity between primary tumors and hepatic metastases but an excellent intra-lesional homogeneity. These findings may be of key importance for patient stratification and the development of personalized strategies against CRC.

3.
J Innate Immun ; 3(4): 420-34, 2011.
Article in English | MEDLINE | ID: mdl-21178331

ABSTRACT

The CD6 scavenger receptor is known to be expressed on virtually all T cells and is supposed to be involved in costimulation, synapse formation, thymic selection and leukocyte migration. Here, we demonstrate that CD6 is differentially expressed by a subpopulation of peripheral CD56(dim) natural killer (NK) cells and absent on CD56(bright) NK cells. CD56(dim)CD16(+) cells represent the major NK subset in the periphery, and most cells within this group are positive for CD6. Most killer immunoglobulin-like receptor- and immunoglobulin-like transcript-positive cells also belong to the CD6(+) subpopulation, as expected from their restricted expression on CD56(dim) NK cells. In addition, CD6(+) NK cells are similar to the newly identified CD94(low)CD56(dim) NK subpopulation and most distant from the recently defined CD27(+) NK subpopulation based on the reverse correlation of expression between CD6 and CD27, a marker associated primarily with CD56(bright) NK cells. With respect to CD6 function on NK cells, direct CD6 triggering did not result in degranulation but induced secretion of cytokines (interferon-γ and tumor necrosis factor-α) and chemokines [CXCL10 (IP-10), CXCL1 (GRO-α)]. Thus, CD6 expression on peripheral NK cells marks a novel CD56(dim) subpopulation associated with distinct patterns of cytokine and chemokine secretion.


Subject(s)
Antigens, CD/metabolism , Antigens, Differentiation, T-Lymphocyte/metabolism , CD56 Antigen/metabolism , Chemokines/metabolism , Cytokines/metabolism , Killer Cells, Natural/metabolism , Receptors, Scavenger/metabolism , Cell Differentiation , Chemokine CXCL1/metabolism , Chemokine CXCL10/metabolism , Humans , Interferon-gamma/metabolism , Killer Cells, Natural/cytology , Killer Cells, Natural/immunology , Tumor Necrosis Factor-alpha/metabolism
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