Defining metrics of visual acuity from theoretical models of observers.

Many experimental studies show that metrics of visual image quality can predict changes in visual acuity due to optical aberrations. Here we use statistical decision theory and Fourier optics formalism to demonstrate that two metrics known in the field of vision sciences are approximations of two different theoretical models of linear observers. The theory defines metrics of visual acuity to potentially predict changes in visual acuity due to optical aberrations, without needing a posteriori scale or offset. We illustrate our approach with experiments, using combinations of defocus and spherical aberration, and pure coma.

Absolute prediction of relative changes in contrast sensitivity with aberrations using a single metric of retinal image quality.

Metrics of retinal image quality predict optimal refractive corrections and correlate with visual performance. To date, they do not predict absolutely the relative change in visual performance when aberrations change and therefore need to be a-posteriori rescaled to match relative measurements. Here we demonstrate that a recently proposed metric can be used to predict, in an absolute manner, changes in contrast sensitivity measurements with Sloan letters when aberrations change. Typical aberrations of young and healthy eyes (for a 6 mm pupil diameter) were numerically introduced, and we measured the resulting loss in contrast sensitivity of subjects looking through a 2 mm diameter pupil. Our results suggest that the metric can be used to corroborate measurements of visual performance in clinical practice, thereby potentially improving patient follow-ups.

Predicting the effects of defocus blur on contrast sensitivity with a model-based metric of retinal image quality.

We measure the effect of defocus blur on contrast sensitivity with Sloan letters in the 0.75-2.00 arc min range of letter gaps. We compare our results with the prediction of the Dalimier and Dainty model [J. Opt. Soc. Am. A25, 2078 (2008)JOAOD60740-323210.1364/JOSAA.25.002078] and propose a new metric of retinal image quality that we define as the model limit for very small letters. The contrast sensitivity is measured for computationally blurred Sloan letters (0, 0.25, and 0.50 diopters for a 3 mm pupil) of different sizes (20/40 to 20/15 visual acuity), and subjects look through a small (2 mm) diaphragm to limit the impact of their own aberration on measurements. Measurements and model predictions, which are normalized by the blur-free condition, weakly depend on letter size and are in good agreement with our metric of retinal image quality. Our metric relates two approaches of modeling visual performance: complete modeling of the optotype classification task and calculation of retinal image quality with a descriptive metric.

The random walk of accommodation fluctuations.

The focusing distance of the eye fluctuates during accommodation. However, the visual role of these accommodation fluctuations is not yet fully understood. The fluctuation complexity is one of the obstacles to this long standing challenge in visual science. In this work we seek to develop a statistical approach that i) accurately describes experimental measurements and ii) directly generates randomized and realistic simulations of accommodation fluctuations for use in future experiments. To do so we use the random walk approach, which is usually appropriate to describe the dynamics of systems that combine both randomness and memory.

Correlation between Contrast Sensitivity and Modulation Transfer Functions.

SIGNIFICANCE: Previous studies found no correlation between visual acuity and optical quality in a population of young subjects with good vision. Using sinusoidal gratings, we systematically investigate the correlation between contrast sensitivity and optical quality as a function of spatial frequency. PURPOSE: This study describes the correlation between the contrast sensitivity function (CSF) and the modulation transfer function (MTF) in a sample of young and informed subjects. Our results are compared with prior studies on the correlation between visual acuity and metrics of image quality. We also compare our results with previous studies that compare the CSF, the MTF, and the neural contrast sensitivity function (NCSF). METHODS: The CSF of 28 informed subjects is measured in photopic conditions. The polychromatic MTF is computed from the measurements of monochromatic aberrations. The (CSF, MTF) correlation is estimated as the Pearson correlation coefficient, at each spatial frequency. The NCSF of each subject is estimated as the ratio of CSF to MTF. RESULTS: We obtain high correlation coefficients (0.8) in the range of spatial frequencies of 3 to 6 cycles per degree, which also corresponds to high NCSF. Correlation decreases with increasing spatial frequency in the range of 6 to 18 cycles per degree (down to 0.0 at 18 cycles per degree). In that range, optical and neural contrast sensitivities are both approximately reduced by factor 4. CONCLUSIONS: In our sample of young subjects with good vision, the CSF with sinusoidal gratings better differentiates eyes of good optical quality at intermediate spatial frequencies (3 to 6 cycles per degree) than at higher spatial frequencies (12 to 18 cycles per degree). At the highest tested spatial frequency of sinusoidal gratings (18 cycles per degree), there is no significant correlation between optical quality and contrast sensitivity.

Intracellular dynamics measurements with full field optical coherence tomography suggest hindering effect of actomyosin contractility on organelle transport.

Intracellular motion can be quantitatively monitored in tissues using coherence-gated microscopic techniques. With full-field optical coherence tomography (FFOCT), the use of high numerical aperture microscope objectives provides a high resolution mapping of intracellular dynamics that are probed with subwavelength sensitivity. In the upper temporal bandwidth that we have used (1-6 Hz) the main contribution to the dynamic signal arises from the overall dynamical, optically heterogeneous cytoplasm. We propose a method to specifically study the impact of actomyosin contractility on the intracellular dynamic signal by performing high throughput, comparative measurements of multicellular aggregates with and without blebbistatin action, a selective inhibitor of class-II myosins that disrupts actomyosin contractile activity. Our results indicate a significant increase in the fraction of the signal that decorrelates within 1 second after inhibition of contractility. This observation mitigates the anticipated importance of actomyosin contractile forces to directly move organelles, but highlights their role in hindering organelle transport via their stiffening effect of the viscoelastic cytoplasm.

Fluorescent correlation spectroscopy measurements with adaptive optics in the intercellular space of spheroids.

In this study we demonstrate the use of adaptive optics to correct the biasing effects of optical aberrations when measuring the dynamics of molecules diffusing between cells in multicellular spheroids. Our results indicate that, on average, adaptive optics leads to a reduction of the 3D size of the point spread function that is statistically significant in terms of measured number of molecules and diffusion time. The sensorless approach, which uses the molecular brightness as optimization metric, is validated in a complex, highly heterogeneous, biological environment. This work paves the way towards the design of accurate diffusion measurements of molecules in thick biological specimens.

Correction of cell-induced optical aberrations in a fluorescence fluctuation microscope.

We describe the effect of optical aberrations on fluorescence fluctuations microscopy (FFM), when focusing through a single living cell. FFM measurements are performed in an aqueous fluorescent solution and prove to be a highly sensitive tool to assess the optical aberrations introduced by the cell. We demonstrate an adaptive optics (AO) system to remove the aberration-related bias in the FFM measurements. Our data show that AO is not only useful when imaging deep in tissues but also when performing FFM measurements through a single cellular layer. This work paves the way for the application of FFM to complex three-dimensional multicellular samples.

MNOS stack for reliable, low optical loss, Cu based CMOS plasmonic devices.

We study the electro optical properties of a Metal-Nitride-Oxide-Silicon (MNOS) stack for a use in CMOS compatible plasmonic active devices. We show that the insertion of an ultrathin stoichiometric Si(3)N(4) layer in a MOS stack lead to an increase in the electrical reliability of a copper gate MNOS capacitance from 50 to 95% thanks to a diffusion barrier effect, while preserving the low optical losses brought by the use of copper as the plasmon supporting metal. An experimental investigation is undertaken at a wafer scale using some CMOS standard processes of the LETI foundry. Optical transmission measurments conducted in a MNOS channel waveguide configuration coupled to standard silicon photonics circuitry confirms the very low optical losses (0.39 dB.µm(-1)), in good agreement with predictions using ellipsometric optical constants of Cu.

Adaptive optics for fluorescence correlation spectroscopy.

Fluorescence Correlation Spectroscopy (FCS) yields measurement parameters (number of molecules, diffusion time) that characterize the concentration and kinetics of fluorescent molecules within a supposedly known observation volume. Absolute derivation of concentrations and diffusion constants therefore requires preliminary calibrations of the confocal Point Spread Function with phantom solutions under perfectly controlled environmental conditions. In this paper, we quantify the influence of optical aberrations on single photon FCS and demonstrate a simple Adaptive Optics system for aberration correction. Optical aberrations are gradually introduced by focussing the excitation laser beam at increasing depths in fluorescent solutions with various refractive indices, which leads to drastic depth-dependent bias in the estimated FCS parameters. Aberration correction with a Deformable Mirror stabilizes these parameters within a range of several tens of µm into the solution. We also demonstrate, both theoretically and experimentally, that the molecular brightness scales as the Strehl ratio squared.