ABSTRACT
Eleven bioxanthracenes and two monomers, six novel in nature, were isolated from the insect pathogenic fungus Cordyceps pseudomilitaris BCC 1620. Growth optimization of the strain led to the improvement of bioxanthracenes production. The bioxanthracenes were evaluated for their antimalarial activity and cytotoxicity.
Subject(s)
Anthracenes/isolation & purification , Antimalarials/isolation & purification , Antimalarials/pharmacology , Hypocreales/classification , Hypocreales/metabolism , Animals , Anthracenes/metabolism , Anthracenes/pharmacology , Antimalarials/metabolism , Cell Line , Fermentation , Humans , Hypocreales/pathogenicity , Inhibitory Concentration 50 , Insecta/microbiology , Microbial Sensitivity Tests , Plasmodium falciparum/drug effects , Toxicity TestsABSTRACT
Bioassay-guided fractionation of the extract from the wood-decayed fungus Xylaria sp. BCC 1067 led to the isolation of five antiplasmodial compounds, (-)-depudecin, (+)-phaseolinone, (+)-phomenone, 19,20-epoxycytochalasin Q, and (E)-methyl 3-(4-methoxyphenoxy)propenoate. These structures were elucidated using spectroscopic methods, especially NMR analysis.
Subject(s)
Antimalarials/isolation & purification , Antineoplastic Agents/isolation & purification , Ascomycota/chemistry , Alkadienes/chemistry , Alkadienes/isolation & purification , Alkadienes/pharmacology , Animals , Antimalarials/chemistry , Antimalarials/pharmacology , Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Chromatography, High Pressure Liquid , Epoxy Compounds/chemistry , Epoxy Compounds/isolation & purification , Epoxy Compounds/pharmacology , Fatty Alcohols/chemistry , Fatty Alcohols/isolation & purification , Fatty Alcohols/pharmacology , Humans , Magnetic Resonance Spectroscopy , Naphthols/chemistry , Naphthols/isolation & purification , Naphthols/pharmacology , Plasmodium falciparum/drug effects , Sesquiterpenes/chemistry , Sesquiterpenes/isolation & purification , Sesquiterpenes/pharmacology , Tumor Cells, CulturedABSTRACT
Bioassay-guided fractionation of an extract from the fungus Myrothecium verrucaria BCC 112 resulted in the first isolation of roridin E acetate (5) from nature together with four common macrocyclic trichothecene isomers (1-4). Trichothecenes 1-5, while known as mycotoxins, were evaluated for their high antimalarial activity.
