High-resolution sub-Doppler infrared spectroscopy of atmospherically relevant Criegee precursor CH2I radicals: CH2 stretch vibrations and "charge-sloshing" dynamics.

The combination of a pulsed supersonic slit-discharge source and single-mode difference frequency direct absorption infrared spectroscopy permit first high resolution infrared study of the iodomethyl (CH2I) radical, with the CH2I radical species generated in a slit jet Ne/He discharge and cooled to 16 K in the supersonic expansion. Dual laser beam detection and collisional collimation in the slit expansion yield sub-Doppler linewidths (60 MHz), an absolute frequency calibration of 13 MHz, and absorbance sensitivities within a factor of two of the shot-noise limit. Fully rovibrationally resolved direct absorption spectra of the CH2 symmetric stretch mode (ν2) are obtained and fitted to a Watson asymmetric top Hamiltonian with electron spin-rotation coupling, providing precision rotational constants and spin-rotation tensor elements for the vibrationally excited state. Analysis of the asymmetric top rotational constants confirms a vibrationally averaged planar geometry in both the ground- and first-excited vibrational levels. Sub-Doppler resolution permits additional nuclear spin hyperfine structures to be observed, with splittings in excellent agreement with microwave measurements on the ground state. Spectroscopic data on CH2I facilitate systematic comparison with previous studies of halogen-substituted methyl radicals, with the periodic trends strongly correlated with the electronegativity of the halogen atom. Interestingly, we do not observe any asymmetric CH2 stretch transitions, despite S/N ≈ 25:1 on strongest lines in the corresponding symmetric CH2 stretch manifold. This dramatic reversal of the more typical 3:1 antisymmetric/symmetric CH2 stretch intensity ratio signals a vibrational transition moment poorly described by simple "bond-dipole" models. Instead, the data suggest that this anomalous intensity ratio arises from "charge sloshing" dynamics in the highly polar carbon-iodine bond, as supported by ab initio electron differential density plots and indeed consistent with observations in other halomethyl radicals and protonated cluster ions.

[Contemporary technicqes in diagnostic patients with vaginal microbiota]. / Soucasné vyuzití technik v diagnostice recidivujícího vulvovaginálního dyskomfortu.

OBJECTIVE: Review of literature dealing with the specificity of the vagina from the standpoint of microbial colonization. DESIGN: Literature review. SETTING: Department of Obstetrics and Gynecology, Department of Clinical Microbiology, University Hospital and Medical Faculty in Hradec Kralove, Charles University Prague. METHODS: Analysis of the literature review and personal experience dealing with vaginal microbiota.

Necrotic fasciitis of the lateral muscle group of the thigh following outside-in transobturatory tape procedure.

OBJECTIVE: Sling procedures are the preferred surgical treatment of stress urinary incontinence (SUI) due to their high success rate and low risk of complications. DESIGN: Case report. SETTING: Department of Obstetrics and Gynecology, Medical Faculty of Charles University and Faculty Hospital Hradec Kralove. CASE REPORT: Necrotic fasciitis is a rare infectious complication, the mortality of which varies by age and can reach 73% if untreated. We present a case of a rare yet serious complication, necrotic fasciitis (NF), of a lateral muscle group of the thigh following antiincontinence implantation. Polypropylene transobturatory midurethral outside-in tape (TOT) was inserted, and NF occurred shortly after surgery. CONCLUSION: Effective treatment was based on a timely diagnosis, administration of wide-spectrum antibiotics, intensive care unit support, surgical treatment, and multidisciplinary cooperation. Timely initiation and adequate treatment are critical in preventing mortality.

[Amniotic fluid interleukin 6 levels in preterm premature rupture of membranes]. / Interleukin 6 v plodové vode pacientek s predcasným odtokem plodové vody.

OBJECTIVE: The purpose of this study was to determinate the changes of amniotic fluid interleukin 6 (IL-6) concentrations in patients with preterm premature rupture of the membranes (PPROM), and in the presence of microbial invasion of the amniotic cavity (MIAC) and histological chorioamnionitis (HCA). The aim was to examine amniotic fluid IL-6 in relation to MIAC and HCA. DESIGN: Prospective study. SETTING: Department of Obstetrics and Gynecology Medical Faculty Charles University Hradec Králové. METHODS: We studied 37 women between 24 and 36 weeks of gestation with PPROM. Samples of amniotic fluid were collected by transabdominal amniocentesis. Polymerase chain reaction for the genital mycoplasmas and culture for aerobic and anaerobic bacteria were performed. Twenty-eight of 37 patients placentas were collected and assessed for presence or absence HCA. IL-6 concentration in amniotic fluid were determined using a sensitive and specific diagnostic kit Human IL-6 Quantikine ELISA manufactured R&D Systems, USA. RESULTS: There was significant difference in the median amniotic fluid IL-6 concentration between patients with preterm rupture of the membranes with and without MIAC and HCA (patients with MIAC and HCA: median 915 pg/ml, range 651-1854 pg/ml vs. patients without MIAC and HCA: median 780 pg/ml, range 184-1059 pg/ml; p=0.047). There was no significant difference in the median amniotic fluid IL-6 concentration between patients with preterm rupture of the membranes with and without MIAC (patients with MIAC: median 915 pg/ml, range 195-1854 pg/ml vs. patients without MIAC: median 792 pg/ml, range 184-1993 pg/ml; p=0.53). There was no significant difference in the median amniotic fluid IL-6 concentration between patients with preterm rupture of the membranes with and without HCA (patients with HCA: median 829 pg/ml, range 195-1992 pg/ml vs. patients without HCA: median 768 pg/ml, range 184-1890 pg/ml; p = 0.31). CONCLUSION: Amniotic fluid IL-6 concentrations patients with PPROM with presence HCA and MIAC were significantly higher than IL-6 concentration patients without HCA and MIAC.

Group-specific component (GC) and transferrin (TF) subtypes in psoriatic patients.

Frequency of occurrence of TFC and GC subtypes and determining genes was evaluated in 90 and 68 nonrelated psoriatic patients, respectively. The obtained distributions of frequencies were compared with those recorded in healthy population. No statistically significant differences were observed.

[Examination of the correlation between transferrin C and atopic dermatitis]. / Badanie korelacji pomiedzy podtypami transferyny C a atopowym zapaleniem skóry.

Transferrin subtypes are considered to be some of genetic markers which can correlate with the incidence of certain diseases. The present study was designed to find out whether such a correlation is true for atopic dermatitis. Transferrin C subtypes (Tf CI, Tf C2, Tf C3) in blood serum were determined by the method of isoelectric focussing in polyacrylamide gel. Examinations were made in 30 atopic dermatitis patients. The control group was made up by a Polish population sample of 728 normal persons of both sexes. The results obtained were analysed statistically by means of test chi 2. There was no correlation between Tf C and atopic dermatitis (chi 2 = 0,638, d.f. = 2, 0,750 greater than p greater than 0,500).

Subtypes of the Gc gene (vitamin D binding protein) in the Polish population.

Gc subtypes were analyzed by isoelectric focusing on thin-layer polyacrylamide gel. In the sample of the Polish population including 278 persons, six Gc phenotypes were encountered: 1S, 1F-1S, 1F, 2-1S, 2-1F, 2 with the following frequencies: 0.342, 0.151, 0.014, 0.360, 0.072 and 0.061. Frequency of Gc1S was 0.597, Gc1F - 0.126 and Gc2 - 0.277. It was shown that a group system of the Gc protein in the Polish population was in good agreement with Hardy Weinberg equilibrium.

Genetic polymorphism of phosphoglycolate phosphatase (PGP) (E.C.:3.2.3.18) in the Polish population.

In a sample of the Polish population of 261 unrelated adults, six types of phosphoglycolate phosphatase (PGP) were encountered. From the distribution of phenotypes obtained, the following frequency of genes was calculated: PGP1 = 0.8429, PGP2 = 0.1399 and PGP3 = 0.0172. PGP types were also determined in 12 families with 30 children and in 55 mother-child pairs. In the group of children the observed distribution of types was consistent with assumed types. The usefulness of this system in disputed paternity cases is as high as 13.52%.

Inheritance of TfC subtypes in families and mother-child pairs.

Using the method of isoelectric focusing on polyacrylamide gel, TfC subtypes were determined in 90 families with 173 children in 242 mother-child pairs. The results obtained have confirmed the hypothesis that transferrin phenotypes are determined by a single autosomal locus in which multiple alleles are situated.

Transferrin subtypes in the Polish population.

Transferrin subtypes were determined by isoelectric focusing on a thin-layer polyacrylamide gel, using a mixture of ampholine of three pH ranges. In a sample of the Polish population numbering 361 subjects, six Tf subtypes dependent on three alleles Tfcc1, Tfcc2, Tfcc3 were encountered. They occurred with the following frequency: 0.744, 0.212, 0.044. It was found that Tf system in the Polish population was in a good agreement with Hardy Weinberg equilibrium.