The combined effects of treated sewage discharge and land use on rivers.

Freshwater ecosystems are increasingly threatened by multiple anthropogenic stressors. Release of treated sewage effluent and pollution from agricultural or urban sources can independently reduce water quality with implications for ecological communities. However, our knowledge of the combined effects of these stressors is limited. We performed a field study to quantify the combined effect of treated sewage discharge and land use on nutrient concentrations, sewage fungus presence and communities of macroinvertebrates and benthic algae. Over three seasons in four rivers we found that a model which included an interaction between sewage pollution and time of the year (i.e. months) was the best predictor of nutrient concentrations and the abundance of algae and sewage fungus. Both macroinvertebrate and algae communities shifted downstream of sewage input. Specifically, more tolerant groups, such as cyanobacteria and oligochaetes, were more abundant. The EPT (Ephemeroptera, Plecoptera and Tricoptera) water quality score was best explained by an interaction between month and agriculture in the surrounding landscape. Overall, our results show that sewage discharge has a significant impact on water quality and benthic riverine communities, regardless of the surrounding land uses. Agricultural inputs, however, could be more important than treated sewage discharge in reducing the abundance of sensitive invertebrate taxa. We need both improvements to wastewater treatment processes and reductions in agricultural pollution to reduce threats to vulnerable freshwater communities.

10 kHz laser-induced schliere anemometry for velocity, Mach number, and static temperature measurements in supersonic flows.

A new, to the best of our knowledge, technique for measuring velocity and Mach number in freestream flow is discussed and demonstrated. The technique, laser-induced schliere anemometry, uses a laser to write a laser-induced schliere in the flow, which can then be imaged using high-speed schlieren imaging. Here, we use a laser-induced plasma from the focusing of nanosecond-duration laser pulses from a pulse burst laser to write the disturbance. The resulting localized index of refraction gradient left from the plasma is tracked well beyond the plasma emission lifetime using schlieren imaging, and velocity is found from tracking or through a simple correlation analysis. The blast wave is also used to independently determine the Mach number via the Mach cone effect, which provides information about the mean static temperature. This technique shows great potential for use in characterizing freestream flow in supersonic facilities and is demonstrated here in a Mach 2 blowdown facility and a Mach 4 Ludwieg tube.

Targeted Vagus Nerve Stimulation does not Disrupt Cardiac Function in the Diabetic Rat.

In this study, we acutely identified a target branch of the vagus nerve known as the pancreatic branch of the vagus nerve, which exclusively innervates the pancreas by applying electrical stimulus to the known cervical vagus nerve and observing compound neural action potentials at the target nerve. In a set of chronically implanted rats, the target nerve was again cuffed using an electrode and also implanted with a continuous glucose monitor. A model of type 1 diabetes (T1D) was chemically induced and hyperglycemic state confirmed. After induction, stimulation was applied to the pancreatic branch of the vagus nerve and heart rate variability measured to assess the targeted nature of the stimulation. Pancreatic vagus nerve stimulation in a diabetic model was not found to influence heart rate demonstrating the ability of targeted stimulation to be used as for organ-specific neuromodulation while minimizing side effects.