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Immunological identification of the mouse H4 histamine receptor on spinal cord motor neurons using a novel anti-mouse H4R antibody.

Lethbridge, N L; Chazot, P L.

Inflamm Res ; 59 Suppl 2: S197-8, 2010 Mar.

Article in English | MEDLINE | ID: mdl-20020316

ABSTRACT

In this report, we describe the development of the first anti-mouse H(4) receptor antibody. Utilising this new immunological probe, new evidence is provided for oligomeric mH(4)Rs and the presence of H(4) receptors on a subpopulation of murine motor neurons. This expands the cell types where the H(4) receptor is expressed in the mammalian CNS.

Subject(s)

Antibodies, Blocking/pharmacology , Motor Neurons/metabolism , Receptors, G-Protein-Coupled/metabolism , Receptors, Histamine/metabolism , Spinal Cord/metabolism , Amino Acid Sequence , Animals , Antibody Specificity , Blotting, Western , Immunohistochemistry , Mice , Molecular Sequence Data , Receptors, G-Protein-Coupled/immunology , Receptors, Histamine/immunology , Receptors, Histamine H4 , Spinal Cord/cytology , Transfection

Generation and characterisation of the first anti-human H3R445/453 isoform specific antibody probe.

Lethbridge, N L; Shenton, F C; Chazot, P L.

Inflamm Res ; 58 Suppl 1: 43-4, 2009 Apr.

Article in English | MEDLINE | ID: mdl-19271149

Subject(s)

Antibodies/metabolism , Molecular Probes , Protein Isoforms/metabolism , Receptors, Histamine H3/metabolism , Animals , Antibodies/genetics , Cell Line , Humans , Protein Isoforms/genetics , Rabbits , Rats , Receptors, Histamine H3/genetics

ABSTRACT

Subject(s)

Subject(s)

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