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J Bacteriol ; 185(11): 3469-72, 2003 Jun.
Article in English | MEDLINE | ID: mdl-12754247

ABSTRACT

The frequencies of nonselected mutations among adaptive Lac(+) revertants of Escherichia coli strains with and without the error-prone DNA polymerase IV (Pol IV) were compared. This frequency was more than sevenfold lower in the Pol IV-defective strain than in the wild-type strain. Thus, the mutations that occur during hypermutation are due to Pol IV.


Subject(s)
Adaptation, Physiological , DNA Polymerase beta/metabolism , Escherichia coli/enzymology , Mutation , Culture Media , Escherichia coli/genetics , Escherichia coli/growth & development , Escherichia coli Proteins/genetics , Escherichia coli Proteins/metabolism , Lactose/metabolism
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