[The effect of irradiation on the inner laryngeal lymphatics (author's transl)]. / Der Bestrahlungseffekt auf die Lymphkapillaren des Kehlkopfes.

In a combined lymphangioscopical and ultrastructural examination 7 months post telecobalt 60 irradiation with 60 Gy, the morphological and functional aspects of the inner laryngeal lymph capillaries were studied. The prominent feature of the morphological changes was the endothelial damage of the lymphatic capillaries including swelling, vacuolisation and necrosis. Many lymph vessels were dilated but patent. A few capillaries where collapsed and surrounded by increased amounts of fibrous tissue, some capillaries where lacking their endothelial lining. So degeneration of the endothelial cell i.e. of the endothelial junctions and focal increase of interstitial collagen with resulting stenosis of the capillaries may be observed. The clinical and functional aspects of the morphological findings are discussed.

[Ultrastructural morphometric changes in spiral ganglion cells of the rat after broad band noise (author's transl)]. / Ultrastrukturell-morphometrische Veränderungen des Ganglion spirale cochleae bei der Ratte nach Beschallung.

The comparison of ultrastructural and ultrastructural-morphometric findings in spiral ganglion cells subsequent to broad noise with 105 dB SPL over a period of 40 min demonstrates that there are morphometric changes of the cytoarchitecture of granular ganglion cells even without evident ultrastructural changes. Morphometric analyses shows a significant extension of the volume proportion of mitochondria per unit volume cytoplasm. The volume and the surface of the rough endoplasmic reticulum, the volume of the free ribosomes and the average single volume of the nucleoli increase per granular ganglion cell. The physiologic significance of these changes is discussed. The results show that changes of the cytoarchitecture of granular spiral ganglion cells are earlier detected by morphometry than by ultrastructural description.

[Influence of different fixation buffers on the quantitative cytoarchitecture of the cochlear spiral ganglion. A morphometric study (author's transl)]. / Einfluss verschiedener Pufferlösungen auf die Strukturerhaltung des Ganglion spirale cochleae. Eine morphometrische Studie.

The influence of two fixation buffers on the quantitative cytoarchitecture of the cochlear spiral ganglion in guinea pigs was evaluated morphometrically. After fixation with phosphate buffered 1.3% OsO4 granular spiral ganglion cells lost 45% of their average individual volume as compared to the volume after fixation with s-collidine buffered 1.3% OsO4. Using the two fixatives there was no significant difference of the volume proportion of cell nuclei, mitochondria, lysosomes and rough endoplasmic reticulum per unit volume cytoplasm of the granular spiral ganglion cells. The volume proportion of their ribosomes and their Golgi apparatus per unit volume cytoplasm increased 3.5fold after fixation with phosphate buffered OsO4. The volume density of the granular ganglion cells decreased by 30%, the volume density of the interganglionar space (= space between granular ganglion cells) showed an increase of 50% using phosphate buffer. Mostly the extracellular space was participating in this relative increase of the interganglionar space. As a result fixation of the spiral ganglion for morphometric studies should be performed using s-collidine buffered OsO4. The morphometric findings underline the presumption of semicompact myelin being a fixation artefact.