[Biological properties of a prolonged action interferon inductor].

AIM: Evaluation of composite formulation of yeast double stranded RNA with polyglucinum (dsRNA-PG) effect on non-specific antiviral resistance factors in mice in comparison with commercial formulation Ridostin. MATERIALS AND METHODS: dsRNA and Ridostin formulations were injected intramuscularly once at the dose of 5 mg/ml, polyglucinum--at the dose of 3.75 mg/ml. 3, 5, 24, 48 and 72 hours after the injection serum interferon levels, neutrophil oxidation-reduction activity parameters, peritoneal macrophage phagocyte activity levels were analyzed in mice blood samples. RESULTS: New dsRNA and polyglucinum containing composite formulation is a non-specific resistance system stimulator. dsRNA-PG effect on interferon synthesis and mice phagocyte activity was higher than with Ridostin and developed earlier. Neutrophil function activation by the formulation had a prolonged effect. A possible explanation for increased activity of dsRNA and polyglucinum composite formulation is a modulating effect by the polysaccharide component. CONCLUSION: The new formulation may have a more intensive and prolonged protective effect against influenza virus in comparison with Ridostin.

[The impact of compound of double-stranded RNA and hyaluronic acid on the parameters of non-specific resistance in mice]. / Vliianie kompozitsii dvuspiral'nykh RNK i gialuronovoi kisloty na pokazateli nespetsificheskoi ustoichivosti myshei.

The effect of yeast double-stranded RNA (dsRNA) and of hyaluronic acid (HA) compositions produced on the interferon synthesis, peritoneal phagocytic activity of macrophages and on the hematological parameters were studied in an experiment with white noninbred mice. HA was shown to enhance the dsRNA-induced interferon synthesis, to inhibit the leukopenic reaction and to produce no effect on the phagocytosis-stimulating activity. The data obtained are indicative of that HA is a promising preparation regarding its use within the interferon-inducing compositions based on dsRNA.

[Features of formation of antiviral resistance upon local application of the interferon inducer ridostin]. / Osobennosti formirovaniia protivovirusnoi ustoichivosti pri mestnom primenenii induktora interferona ridostina.

Antiviral potency of an ointment dosage form of interferon (IF) inducer ridostin has been demonstrated in guinea pigs with genital herpes. Early beginning of therapy led to a 72% decrease of the intensity of clinical signs with cure 12-13 days earlier than in the control. Application of ridostin ointment onto the skin of intact animals caused an increase of IF level in the blood and of the phagocytic activity of peritoneal exudation macrophages. Ridostin effect on the total-systems factors of nonspecific defense appears to be one mechanism in the formation of antiviral resistance upon external application of this IF inducer.

[Preparation of deoxyribonucleic acid (DNA) form salmon milt by the phenol method]. / Poluchenie preparatov dezoksiribonukleinovoi kisloty (DNK) iz molok lososevykh ryb fenol'nym metodom.

The paper presents a modification of the Kirby method for the DNA preparation from salmon milt. The modified procedure includes ethanol pretreatment and subsequent homogenization in 0.01 M. Trylon B. The paper describes the conditions for DNA purification through alkaline treatment and renaturation at 66 degrees C. The modified method can be used to obtain two preparations of DNA viscous solution in water (DNA homogenate), employed as a raw material in the preparation of 5-deoxyribonucleotides, and a dry preparation.