The Effect of Therapeutic Meibomian Glands Expression on Evaporative Dry Eye: A Prospective Randomized Controlled Trial.

PURPOSE: To determine the clinical benefits of Meibomian gland expression therapy for the treatment of dry-eye disease caused by Meibomian gland dysfunction (MGD). METHODS: In a prospective randomized controlled double-masked trial, 87 eyes of 44 patients with MGD and dry-eye symptoms were enrolled. Patients were randomly assigned into two groups; a study group that received therapeutic Meibomian gland expression once every month, and a control group that received sham treatment. All patients received treatment with artificial tears. RESULTS: One week after the first treatment, the Ocular Surface Disease Index (OSDI) score improved significantly in the study group (mean change -18.5 ± 21.2, p = .01) but not in the control group (-3.8 ± 15.8, p = .16); after 1 month, both groups improved significantly (-20.5 ± 19 p = .001 in the study group and -6.5 ± 11, p = .016 in the control group). The improvement continued at 2 months in the study group (-28.4 ± 26.1, P < .0001) and in the control group (-9.6 ± 9.9, p = .007). The blepharitis questionnaire score improved in the study group compared to controls after 1 week (-9.95 ± 12.52 versus -1.77 ± 9.1, p = .03) 1 month (-11.5 ± 10.9 versus -1.1 ± 9.4, p = .02) and 2 months (-16.5 ± 8.0 versus -8.8 ± 11.7, p = .02). Burning sensation was significantly reduced only in the study group. Mean change after 2-month treatment was -2.00 ± 1.2583 (p < .0001) vs -0.67 ± 1.44 (p = .08). The trend was similar in Eyelid scales. Conjunctival hyperemia improved only in the study group 1 week after the treatment (-0.12 ± 0.32 p = .03). CONCLUSION: Therapeutic Meibomian gland expression improves dry-eye symptoms in subjects with MGD, compared to conventional treatment with artificial tears.

The association of keratoconus with blepharitis.

BACKGROUND: Identifying potentially treatable risk factors for the progression of keratoconus is of great importance. The purpose of this study was to determine the association between blepharitis and keratoconus. METHODS: In this prospective, comparative, observational study, 50 keratoconus participants from the central district of the Clalit Health Services Health Maintenance Organization in Israel underwent comprehensive eye examination. Seventy-two healthy medical personnel of similar ages were randomly chosen as a control group. The signs and symptoms of blepharitis in each participant were assessed. A thorough survey of the eyelids (scales and foam on the eyelashes, missing eyelashes and expression of meibomian gland contents) was performed. All participants were required to complete two questionnaires: the Ocular Surface Disease Index questionnaire and a questionnaire regarding symptoms, signs and risk factors for blepharitis. RESULTS: Blepharitis was more common in keratoconus participants than in the control group (24 per cent versus 2.8 per cent, p < 0.001). A higher proportion of keratoconus participants reported rubbing their eyes more than once a day (36 per cent versus 11.1 per cent, p = 0.002) as well as red and tired eyes (12 per cent versus zero per cent, p = 0.009). On external eye examination, signs of blepharitis and meibomian gland dysfunction were found more frequently in the keratoconus group (p < 0.05). CONCLUSIONS: Signs and symptoms of blepharitis occur more often in keratoconus participants than in healthy individuals. Blepharitis-related inflammation and associated eye rubbing may contribute to disease progression.

Correlation between central corneal thickness and myopia.

PURPOSE: To analyze the correlation between central corneal thickness (CCT) and myopia in refractive surgery candidates. MATERIALS AND METHODS: Patients that underwent myopic laser refractive surgery between January 2000 and December 2014 were included. Preoperative CCT was measured by ultrasonic pachymetry, and refractive status determined by manifest and cycloplegic refraction. RESULTS: A total of 30,245 individuals were included. Mean age was 28.2 ± 8.6 years, and 45.9% were female. Mean refractive error was 4.02 ± 2.17 D (range 0.25-19.5), and the mean CCT measurement was 533.5 ± 35.5 µm (range 404-794). Younger individuals showed higher degree of myopia (p = 0.006). No difference in CCT was found between women and men (533.0 ± 35.1 and 533.6 ± 35.9 µm, respectively, p = 0.19). Though CCT showed no correlation with age (p = 0.226) participants above age 40 expressed higher CCT values (p < 0.001). No significant correlation was found between the CCT and cylinder (p > 0.05). An increase in mean keratometry was associated with a decrease in CCT (p < 0.001). There was a direct correlation between the degree of myopia and CCT (r = 0.94, p < 0.001). The result remained the same after adjusting for age group and gender in stepwise backward regression analysis (p < 0.001). CONCLUSION: Central corneal thickness is correlated with the degree of myopia among adults undergoing refractive surgery.

Corneal Breakthrough Haze After Photorefractive Keratectomy With Mitomycin C: Incidence and Risk Factors.

PURPOSE: To identify preoperative and intraoperative factors affecting breakthrough corneal haze incidence after photorefractive keratectomy (PRK) with mitomycin C (MMC). METHODS: In this retrospective study of PRK performed at the Care Vision Refractive Laser Center, Tel Aviv, Israel, a total of 7535 eyes (n = 3854 patients; mean age ± SD, 26 ± 6 years; 55% men) underwent PRK with intraoperative MMC application. Patients with histories of corneal pathology or surgery were excluded. Incidence, time of onset, and corneal haze severity were documented on follow-up of 118 ± 110 days. Eyes were grouped by preoperative refraction [low (≤-3D), moderate (-3D to -6D), or high (>-6D) myopia; low (≤3D) or high (>3D) astigmatism; low or high hyperopia]; by intraoperative time (above or below 40 seconds); and by MMC application time (above or below 40 seconds). The main outcome measures were incidence, onset time, and severity of corneal haze. RESULTS: The haze incidence was 2.1% in eyes with high myopia versus 1.1% in those with low to moderate myopia (P = 0.002), and 3.5 times higher in eyes with high than with low astigmatism (P < 0.05). The overall incidence was higher in eyes treated for hyperopia (10.8%) than for myopia (1.3%) (P = 0.0001). In eyes with moderate myopia, the haze incidence was lower in MMC application time ≥40 seconds (0%) than in <40 seconds (1.3%) (P = 0.03). After surgery, a mild early haze incidence peaked at 68.8 ± 6 days and severe late haze at 115 ± 17 days (P = 0.02). CONCLUSIONS: Hyperopic and large myopic or astigmatic corrections carry higher risk of haze. Longer MMC application might have beneficial haze prevention.

Risk factors predicting steroid-induced ocular hypertension after photorefractive keratectomy.

PURPOSE: To assess the risk factors contributing to steroid-induced ocular hypertension after photorefractive keratectomy (PRK). SETTING: Care Laser Centers, Tel Aviv, Israel. DESIGN: Retrospective case series. METHODS: Patients having PRK between January 2000 and December 2015 were followed for at least 3 months. Intraocular pressure (IOP) was measured using the Goldmann applanation tonometer after 1 week and after 1, 3, and 6 months. Ocular hypertension was defined as an IOP elevation of 25% while on topical steroid treatment (minimum 28 mm Hg) followed by an IOP drop of 25% when steroid treatment was discontinued. RESULTS: The study comprised 1783 patients (3566 eyes). The mean age of the patients was 26.95 years ± 7.56 (SD), and 54.85% were men. A total of 106 eyes (2.97%) were steroid responders. The responder group had a higher proportion of men than the nonresponder group (70.75% versus 29.25%; P < .001), higher central corneal thickness (CCT) (531.9 ± 40.2 µm versus 521.2 ± 40.9 µm; P = .008), lower mean keratometry (K) power (43.39 ± 1.84 diopters [D] versus 44.08 ± 1.88 D; P < .001), higher proportion of high myopia (>6.0 D) (31.13% versus 22.18%; P = .03), and higher rate of postoperative corneal haze (16.98% versus 4.25%; P < .001) and were treated postoperatively with more potent steroids. All factors remained significant in the multivariate analysis. CONCLUSION: Significant factors associated with post-PRK ocular hypertension were male sex, high CCT, a low mean K reading, high myopia, corneal haze, and treatment with stronger steroids such as dexamethasone.

Treatment of adenoviral keratoconjunctivitis with a combination of povidone-iodine 1.0% and dexamethasone 0.1% drops: a clinical prospective controlled randomized study.

PURPOSE: To determine the efficacy of combination povidone-iodine (PVP-I) 1.0% eyedrops and dexamethasone 0.1% eyedrops in the treatment of adenoviral keratoconjunctivitis. MATERIALS AND METHODS: In a prospective, randomized, controlled, double-blinded clinical trial patients with recent adenoviral keratoconjunctivitis (diagnosed clinically and confirmed by PCR), we randomly divided into three treatment groups: study group - received PVP-I 1.0% and dexamethasone 0.1%, control 1 group - received dexamethasone 0.1% and control 2 group - received lubricating eyedrops (hypromellose 0.3%). The treatment was administered four times a day in each group. All patients were examined and filled a questionnaire before treatment and on the 3rd, 5th and 7th days of treatment. RESULTS: We included in the study 78 eyes (26 in each group). Adenovirus type 8 was the most common pathogen (83% of cases). The fastest improvement in patients red eyes, discharge, superficial punctate keratitis and pseudomembranes was observed in the study group (p < 0.001). Those patients reached a near complete recovery in 5-7 days, which was also confirmed by reduction in Adenovirus titres by PCR. The slowest improvement was in the control 2 group. Subepithelial infiltrates (SEI) were observed in 44% of the control 1 group, 20% of the control 2 group and in 0% of the study group. The rate of reduction in Adenovirus titres was the slowest in the control 1 group. CONCLUSION: The combination of PVP-I 1.0% and dexamethasone 0.1% four times a day can reduce symptoms and expedite recovery in epidemic keratoconjunctivitis patients.

Effect of Anisometropia on the Predictability and Accuracy of Refractive Surgery.

PURPOSE: To evaluate the predictability and accuracy of refractive surgery among adults with myopic anisometropia. METHODS: Consecutive cases of myopic eyes that underwent bilateral laser-assisted in situ keratomileusis (LASIK) or bilateral photorefractive keratectomy (PRK) during a 12-year period in a single center were included. Myopic anisometropia was defined as a difference greater than 1.5 D in spherical equivalent between eyes preoperative. RESULTS: A total of 10,046 paired, operated nonamblyopic eyes of 5023 patients were analyzed. Of these, 472 eyes of 236 (4.7%) patients had myopic anisometropia without amblyopia, and 9574 eyes of 4787 patients served as isometropic controls. After refractive surgery, in the anisometropic group the more myopic eye was corrected by +0.47 ± 0.78 (D) more than the contralateral eye (P < 0.0001). Relative to the target refraction, the more myopic eye was overcorrected by 0.21 ± 0.79 D compared with an undercorrection of 0.16 ± 0.60 D in the less myopic contralateral eye (P < 0.0001) and compared with an undercorrection of 0.15 ± 0.62 D in the isometropic controls (P < 0.0001). Additionally, the variability in the correction of the more myopic eyes was significantly higher compared with the less myopic contralateral eyes and isometropic controls (P < 0.0001). These trends were evident both in PRK and LASIK treatments. The effect of anisometropia was found to be independent of the magnitude of preoperative myopia or surgeon identity. CONCLUSIONS: The more myopic eye of anisometropes undergoing refractive surgery has lower predictability and accuracy and tends to be overcorrected, whereas the less myopic eye has outcome similar to isometropic controls. These results suggest that refractive surgery nomograms should take into account anisometropia.

Factors Predicting the Need for Retreatment After Laser Refractive Surgery.

PURPOSE: To identify the potential risk factors that increase the likelihood of requiring retreatment after refractive surgery. METHODS: This retrospective study included patients who underwent laser in situ keratomileusis or photorefractive keratectomy between January 2005 and December 2012 at the Care-Vision Laser Centers, Tel-Aviv, Israel. Patients were divided into 2 groups according to whether they underwent additional refractive surgery (retreatment) during the study period. RESULTS: Overall, 41,504 eyes (n = 21,313) were included in the final analysis of this study. Throughout the study period, there was a significant reduction in the 2-year annual retreatment rates with a decline from 4.52% for primary surgeries done in 2005 to 0.18% for surgeries performed in 2012 (quadratic R = 0.96, P < 0.001). The retreatment group had significantly higher preoperative age, maximum keratometric power, sphere, cylinder, and better best-corrected visual acuity. They were more likely to have preoperative hyperopia, photorefractive keratectomy as opposed to laser in situ keratomileusis, intraoperative higher humidity conditions and lower temperature, and higher ablation depths. Significant differences in retreatment rates were found between the 5 high-volume surgeons (>1500 procedures performed) ranging from 0.48 to 3.14% (P < 0.0001). Multiple logistic regression analysis demonstrated that age, astigmatism, hyperopia, temperature, and surgeon's experience all significantly affected the need for retreatment. CONCLUSIONS: The following factors significantly increase the need for refractive retreatment: older preoperative age, higher degrees of astigmatism, hyperopia, colder operating room temperature, and less surgeon experience. Some of these factors may be incorporated into nomograms to reduce future retreatment rates.

Comparison of Three Epithelial Removal Techniques in PRK: Mechanical, Alcohol-assisted, and Transepithelial Laser.

PURPOSE: To compare the visual and refractive results obtained after photorefractive keratectomy (PRK) in patients who underwent one of three different epithelial removal techniques. METHODS: The authors reviewed the medical files of consecutive eyes with myopia and myopic astigmatism that were treated during a 10-year period by mechanical PRK, alcohol-assisted PRK, or transepithelial PRK (in the phototherapeutic keratectomy mode), and observed for more than 1 year. RESULTS: A total of 3,417 patients (3,417 eyes) were included in this study. At 3 and 6 months postoperatively, the outcome of alcohol-assisted PRK was superior both in efficacy (P < .01) and safety (P < .001) to those of both mechanical PRK and transepithelial PRK, which were similar. At more than 1 year postoperatively, the mean efficacy index was still high for alcohol-assisted PRK, but low for the transepithelial PRK, corresponding to a mean uncorrected visual acuity of more than one Snellen line lower than those of the other two techniques (P < .0001). All three techniques showed a regression toward myopia more than 1 year postoperatively, with significant undercorrection obtained in eyes treated with transepithelial PRK (P < .0001). CONCLUSIONS: Significant differences were detected in both the visual outcomes and the refractive results of the three epithelial removal techniques. The long-term outcomes were best for alcohol-assisted PRK.

Factors affecting laser in situ keratomileusis flap thickness: comparison of 2 microkeratome heads.

PURPOSE: To identify the potential factors affect that affect the thickness of corneal flaps created using 2 different microkeratome heads. SETTING: Care-Vision Laser Centers, Tel-Aviv, Israel. DESIGN: Retrospective comparative study. METHODS: The study included eyes in which bilateral laser in situ keratomileusis (LASIK) was performed from January 1, 2005, to December 31, 2012, using an SBK-90 microkeratome head (Group 1) or an M2-90 microkeratome head (Group 2). Inclusion criteria were at least 18 years of age, a stable refraction for 12 months, an intraocular pressure of less than 21 mm Hg, and no history of autoimmune disease, diabetes, ocular surgery, or eye disease. In addition, patients had to cease wearing contact lenses for 2 weeks (rigid lenses) or 4 days (soft lenses) before the preoperative evaluation and before surgery. The disposable blade was used in the right eye first and then reused in the left eye. RESULTS: The study evaluated 6242 eyes of 3121 patients with a mean age of 32.2 years ± 9.8 (SD). Group 1 comprised 2560 eyes (41%), and Group 2 comprised 3682 eyes (59%). The eyes in Group 1 had thinner flaps (P < .001). The right eyes had thicker flaps (P < .001). Flap thickness had a positive correlation with precutting central corneal thickness (CCT) and operating room humidity and a negative correlation with patient age, preoperative sphere and cylinder, and operating room temperature. Statistically significant differences were found in flap thickness between surgeons (P < .001). Larger suction rings created thicker flaps (P < .001). Stepwise regression models accounted for up to 28.1% of the variation in flap thickness. CONCLUSIONS: Factors that were significantly correlated with flap thickness included the precutting CCT, whether the right or the left eye, the microkeratome head used, the surgeon performing the procedure, and the preoperative sphere. These findings might help prevent post-LASIK corneal ectasia. FINANCIAL DISCLOSURE: No author has a financial or proprietary interest in any material or method mentioned.

Monovision surgery in myopic presbyopes: visual function and satisfaction.

PURPOSE: To describe our experience with monovision excimer laser correction in individuals with myopic presbyopia. METHODS: This prospective interventional case series was conducted in a private refractive surgery center on 40 patients with presbyopia aged 40 years and older, who were treated with monovision refractive surgery between 2010 and 2011. The dominant eye was corrected for distance vision and the nondominant eye was corrected for near vision, with anisometropia of ≥1.00 diopters (D). All patients underwent comprehensive objective and subjective visual assessments, including a questionnaire that was filled out preoperatively and at 6 and 12 months postoperatively. The primary outcomes were preoperative and postoperative refraction, binocular visual acuity, stereopsis, contrast sensitivity, glare, and questionnaire results. RESULTS: The 1-year follow-up was completed by 38 patients (95%). Preoperative and 1-year postoperative refraction of the distance eye spherical equivalent (SE), anisometropia SE, and uncorrected visual acuity were -4.05 ± 1.94 and -0.01 ± 0.22 D, 0.45 ± 0.50 and 1.73 ± 0.56 D, and 0.87 ± 0.2 and 0.09 ± 0.11 logMAR, respectively. Best-corrected visual acuity was unchanged. Both mean distance and near stereopsis decreased, from 52 to 142 seconds of arc and from 54 to 57 seconds of arc, respectively. Contrast sensitivity and glare decreased significantly. Patient satisfaction improved from 41.5 ± 30.4% to 85.2 ± 5.0% (range, 40 to 100%) at the 1-year follow-up. CONCLUSIONS: Monovision excimer laser correction provides both effective and satisfactory results and should be considered as an option for individuals with myopic presbyopia suitable for, and interested in, refractive surgery.

Human aqueous humor phosphatase activity in cataract and glaucoma.

PURPOSE: To investigate the presence and activity of protein phosphatase-2A (PPase2A), protein phosphatase-2C (PPase2C), and protein tyrosine phosphatases (PTPs) in the human aqueous humor (AH) of patients with primary open-angle glaucoma (POAG) and cataract and to study the correlation between these phosphatases and the redox state of the AH. METHODS: Eighty-six cataract patients and 29 POAG patients who were scheduled for cataract surgery with or without glaucoma surgery were enrolled in the study. PPase2A, PPase2C, and PTPs levels in AH were measured by enzyme-linked immunosorbent assays, Western blot analyses, and spectral METHODS: The redox state was measured by spectral and fluorescent methods. RESULTS: Phosphatase activity-positive results were significantly higher in AH samples from the POAG group (PP2A χ(2)(1) = 11.754, P < 0.01; PP2C χ(2)(1) = 8.754, P < 0.01; PTP χ(2)(1) = 11.073, P < 0.01). Western blot analysis revealed higher PP2C levels in the AH of glaucoma patients compared with PP2C levels in the AH of cataract patients (P = 0.012). Both oxidized/reduced glutathione ratios and superoxide dismutase levels in the AH were significantly higher in the glaucoma group than in the cataract group. Finally significant correlations were found between PP2A and PP2C, PP2A and PTP, and total antioxidant activity and PTP levels. CONCLUSIONS: There is a statistically significant difference between phosphatase levels in the AH of POAG patients and cataract patients. The phosphatase content of the AH represents tissue pathology, but their presence in the AH may be attributed to cell debris or to active signaling to other molecular events.

Prevalence of Epilepsy among Cataract Patients.

PURPOSE: To evaluate the prevalence of epilepsy among patients undergoing cataract surgery. DESIGN: A retrospective observational case control study. METHODS: We calculated the prevalence of epilepsy among all the patients older than 50 years who underwent cataract surgery (years 2000-2007, n = 12,984) in a district of the largest health maintenance organization in Israel (the Central District of Clalit Health Services) and among 25,968 age and gender matched controls. The database was screened for epilepsy by integrating the clinical and ancillary work-up as well as the drug regimen. The use of anti-epileptic drugs (AEDs) was evaluated among the cataract patients and the controls. The main outcome measure was the prevalence of epilepsy and antiepileptic medical treatment among patients undergoing cataract surgery versus controls. RESULTS: No difference was found in demographics among the groups including age, gender, marriage status, socioeconomic class and living place between the study and control groups (except for patients origin). Epilepsy was found to be significantly more prevalent in patients undergoing cataract surgery. The odds ratio (OR) was 1.3 (95% confidence interval (CI): 1.1-1.6): 1.4 in men (95% CI: 1.1-1.9) and 1.2 in women (95% CI: 1.0-1.6). AEDs, particularly clonazepam (OR = 1.5, 95% CI: 1.1-2.1) and carbamazepine (OR = 1.4, 95% CI: 1.05-1.8), were also used more by cataract patients. Multivariate logistic regression analysis revealed a significant association between cataract surgery and epilepsy (OR 1.26, p < 0.001) as well as diabetes (OR 1.38, p < 0.001), arterial hypertension (OR 1.26, p < 0.001), smoking (OR 1.22, p < 0.001), hyperlipidemia (OR 1.12, p < 0.001), and Ashkenazi origin (OR 0.85, p < 0.001). CONCLUSIONS: Epilepsy is associated with the presence of cataract. Various hypotheses may explain this finding, including a cataractogenic role of AEDs.

Homologous scleral graft for corneal perforation in a child.

PURPOSE: To show the emergency use of preserved homologous sclera for the repair of a perforated cornea in a young child. METHODS: Our case was a 3.5-year-old boy who presented with an acute corneal ulcer and endophthalmitis, which was complicated by a developing corneal melting and perforation of 2.5-mm diameter during pars plana vitrectomy. Because no donor cornea was available, a full-thickness preserved donor sclera was used to close the corneal defect. RESULTS: During the next 8 months, scar tissue formed underneath the scleral graft. The graft was removed, revealing mild opacification at the site of corneal perforation. Three years after surgery, best-corrected visual acuity was 20/60. CONCLUSIONS: In the absence of a corneal button for grafting, preserved homologous sclera may be used for closing a corneal defect.

Retained ophthalmic viscosurgical device material in the capsular bag 6 months after phacoemulsification.

We present a 63-year-old man who developed low-grade endophthalmitis 6 months after phacoemulsification and implantation of a foldable posterior chamber intraocular lens (IOL). The capsular bag was distended, but shallowing of the anterior chamber was not observed. Chemical analysis of the clear fluid aspirated from the capsular bag behind the IOL revealed the presence of an ophthalmic viscosurgical device.

Surgical management of corneal plaques in vernal keratoconjunctivitis: a clinicopathologic study.

PURPOSE: To describe the surgical management and histopathologic and immunohistochemical findings in corneal plaques of shield ulcers in vernal keratoconjunctivitis. PATIENTS AND METHODS: Three children (ages 4, 7.5, and 9) presented with corneal plaques unresponsive to conservative systemic and topical medical treatment. Plaques were scraped under general anesthesia, and soft bandage contact lenses were placed. The excised tissue was evaluated by histopathology and immunohistochemistry. RESULTS: During surgery, plaques were found to extend beyond the ulcer margins. Histopathology revealed granular, deeply-eosinophilic, laminar material, firmly attached to the Bowman layer in all cases. Immunohistochemistry confirmed this to be eosinophil-derived major basic protein (MBP). After surgical removal, complete epithelization was evident within 1-4 weeks in all cases. CONCLUSIONS: Corneal plaque is a rare complication of vernal keratoconjunctivitis. These plaques usually do not resolve with standard conservative measures. Failure to epithelialize may be a result of the plaque material extending below the edges of adjacent epithelium. We suggest that MBP plaques precipitate on the denuded stromal bed, thereby playing a pathogenic role in nonhealing shield ulcers.

Devastating endophthalmitis caused by Serratia marcescens in two recipients after transplantation of corneal grafts from the same donor.

We report two cases of severe endophthalmitis, which were caused by Serratia marcescens, and developed in the immediate postoperative period in two recipients of corneal grafts from the same donor. The cause of the donor's death was massive CVA. He had been on mechanical ventilation for 12 days before he died, and had shown no sign of infectious disease while in the hospital. Vitrectomies were performed in the recipients' eyes on the third day after corneal transplantation. On the same day, and again 1day later, the transplanted eyes were injected intravitreally with vancomycin and ceftazidime. Two months after surgery, both eyes developed phthisis. These cases are similar to other rare reported cases describing the virulence of S. marcescens.