[Features and significance of adhesion of bacteria and fungi of the oral cavity as the initial stage of the formation of a microbial biofilm on dental polymer materials]. / Osobennosti i znachenie adgezii bakterii i gribov polosti rta kak etapa formirovaniya mikrobnoi bioplenki na stomatologicheskikh polimernykh materialakh.

OBJECTIVE: Characteristics of the adhesion of yeast fungi and oral bacteria of various types in vitro to samples of polymeric materials for fixed structures of dental prostheses, obtained using various technologies: adjective digital 3D printing and traditional methods. MATERIAL AND METHODS: Conducted model experiments on the adhesion of bacterial (including the main periodontopic-pathogenic species - P. gingivalis, P. intermedia, etc.) and fungal pathogens (C. albicans) to standard samples of polymer materials NextDent C & B Micro Filled Hybrid («NextDent¼, Netherlands), Detax Freeprint temp UV («Detax¼, Germany), obtained by digital additive 3D printing technology, and Luxatemp Automix Plus («DMG¼, Germany) and Acrytemp («Zhermack¼, Italy) - by the traditional method as a control. Removal of adhering microbes from the material was carried out using an ultrasound machine (exposure time 10 minutes, power 60 kHz). RESULTS: The dependence of the degree of microbial adhesion on the nature of the material and processing technology (3D printing, milling) was established. The materials of NextDent C & B Micro Filled Hybrid and Detax Freeprint temp UV showed high resistance to adhesion of clinical isolates of periodontal pathogenic bacteria and C. albicans fungi. CONCLUSION: The lowest adhesion values for periodontopathogenic species and C. albicans fungi were detected when using samples of materials obtained by 3D printing: NextDent C & B Micro Filled Hybrid and Detax Freeprint temp UV compared to Luxatemp Automix Plus and Acrytemp polymers.

[The preconceptional preparation of the patients presenting with chronic endometritis: the evaluation of the effectiveness of phyto- and physiotherapy]. / Pregravidarnaia podgotovka patsientok s khronicheskim éndometritom: otsenka éffektivnosti fito- i fizioterapii.

BACKGROUND: The necessity of the comparative studies with special reference to the preconceptional preparation of the patients presenting with chronic endometritis is self-evident bearing in mind the lack of common approaches to the preconceptional preparation of the patients with this condition. AIMS: The objective of the present study was the evaluation of the effectiveness of preconceptional with the use phyto- and/or physiotherapies for the treatment of the patients suffering from chronic endometritis. MATERIAL AND METHODS: This prospective study included 309 patients from the couples in the infertile marriage attributable to chronic endometritis. All the patients were divided into three group depending on the choice of the preconceptional preparation modality. Those comprising the first group (n=148) received 2.5 mg in days the aqueous extract of one-sided-wintergreen (Orthilia secunda) at a daily dose of 2.5 mg during three months. The patients of the second group (n=101) were given the combined physiotherapeutic treatment. The patients of the third group (n=60) refused any preconceptional preparation; therefore an expectant strategy was chosen to manage them. Part of patients of the first and second groups (n=30) underwent the immunohistochemical investigation aimed at evaluation of the methods employed in this study to the solution of the infertility problem. The data obtained were treated with the use of the Statistica 11.0 software package. RESULT: The preconceptional preparation of the patients suffering infertility associated with chronic endometritis with the use of the aqueous extract of one-sided-wintergreen in the combination with physiotherapeutic treatment resulted in the development of pregnancy in 72.9% and 81.2% of the participants in the study respectively and its completion with labors in 95.3% and 90.1% of the women respectively. The results of the immunohistochemical study involving the patients treated with the aqueous extract of one-sided-wintergreen alone gave evidence of the reduced frequency of frequency of leukocytic infiltration and leukocytic activity in dynamics of an experiment (CD 20+, CD138+, CD 56+, CD 16+, HLA-DRII+); simultaneously, an augmentation of the reception of endometrium to sex steroids. CONCLUSION: The preconceptional preparation of infertile women with the application of the aqueous extracts of one-sided-wintergreen can be recommended for the patients with chronic endometritis, especially in the presence of contraindications for physiotherapy. Phytotherapy made use of in the present study restored reception of endometrium to estrogens and alleviated the autoimmune manifestations of local immunity.