ABSTRACT
13 cases of uterine carcinosarcomas have been studied by molecular-genetic method. The impairment of some genes (K-RAS gene in the first exon, TP53 in the 5th-9th exons, PTEN in the 5th and 8th exons) has been found out. The loss of heterozygosity of TP53 gene in epithelium and mesenchyme of the uterus has been detected. According to the estimation of impairment the studied tumors was divided into monoclonal and biclonal ones. This stratification could be use as prognostic criteria of uterine carcinosarcomas clinical presentation.
Subject(s)
Carcinosarcoma/genetics , Carcinosarcoma/pathology , PTEN Phosphohydrolase/genetics , Proto-Oncogene Proteins p21(ras)/genetics , Tumor Suppressor Protein p53/genetics , Uterine Neoplasms/genetics , Uterine Neoplasms/pathology , Carcinosarcoma/metabolism , Exons , Female , Humans , Loss of Heterozygosity , PTEN Phosphohydrolase/metabolism , Proto-Oncogene Proteins p21(ras)/metabolism , Retrospective Studies , Tumor Suppressor Protein p53/metabolism , Uterine Neoplasms/metabolismABSTRACT
Clinico-morphological and immunohistochemical examinations were carried out in 46 patients with carcinocarcinoma of the corpus uteri to evaluate p53, PTEN and cyclooxygenase-2 (COX-2) expression in mesenchymal and epithelial cells of tumor. p53 expression in the epithelium and that of COX-2 in both compartments appeared to be unfavorable prognosticators of survival while that of PTEN is a lesser one in significance.
Subject(s)
Biomarkers, Tumor/analysis , Carcinosarcoma/chemistry , Carcinosarcoma/pathology , Cyclooxygenase 2/analysis , PTEN Phosphohydrolase/analysis , Tumor Suppressor Protein p53/analysis , Uterine Neoplasms/chemistry , Uterine Neoplasms/pathology , Adult , Aged , Carcinosarcoma/mortality , Female , Gene Expression Regulation, Neoplastic , Humans , Immunohistochemistry , Middle Aged , Predictive Value of Tests , Prognosis , Survival Analysis , Uterine Neoplasms/mortalityABSTRACT
Results of clinico-morphological and immunohistochemical studies of 44 cases of carcinosarcoma of the uterus are presented in an attempt to provide pathogenetic rationale for prognosticators of the disease. Immunohistochemical reactions were used to evaluate the expression of genes and mediators p53 and PTEN and cyclooxigenase (COX-2) in cells of mesenchyme and epithelium of tumor to gauge the influence of either component on its biological behavior. All patients were divided into two groups depending on whether they revealed expression of p53, PTEN and cyclooxigenase or not. The end results--overall survival and complete remission duration--were compared in both groups. No dominance of either component of carcinosarcoma was established.
