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1.
J Biol Chem ; 281(1): 241-51, 2006 Jan 06.
Article in English | MEDLINE | ID: mdl-16223725

ABSTRACT

During the G0/G1-S phase transition, the timely synthesis and degradation of key regulatory proteins is required for normal cell cycle progression. Two of these proteins, c-Myc and cyclin E, are recognized by the Cdc4 E3 ligase of the Skp1/Cul1/Rbx1 (SCF) complex. SCF(Cdc4) binds to a similar phosphodegron sequence in c-Myc and cyclin E proteins resulting in ubiquitylation and degradation of both proteins via the 26 S proteosome. Since the prolyl isomerase Pin1 binds the c-Myc phosphodegron and participates in regulation of c-Myc turnover, we hypothesized that Pin1 would bind to and regulate cyclin E turnover in a similar manner. Here we show that Pin1 regulates the turnover of cyclin E in mouse embryo fibroblasts. Pin1 binds to the cyclin E-Cdk2 complex in a manner that depends on Ser384 of cyclin E, which is phosphorylated by Cdk2. The absence of Pin1 results in an increased steady-state level of cyclin E and stalling of the cells in the G1/S phase of the cell cycle. The cellular changes that result from the loss of Pin1 predispose Pin1 null mouse embryo fibroblasts to undergo more rapid genomic instability when immortalized by conditional inactivation of p53 and sensitizes these cells to more aggressive Ras-dependent transformation and tumorigenesis.


Subject(s)
Cell Transformation, Neoplastic , Cyclin E/metabolism , Fibroblasts/physiology , Genomic Instability/physiology , Peptidylprolyl Isomerase/metabolism , Animals , Cell Line , Cyclin-Dependent Kinase 2/metabolism , Female , Fetus , Fibroblasts/cytology , G1 Phase/physiology , Genes, ras/physiology , Humans , Kidney/cytology , Mice , Mice, Inbred C57BL , Mice, Mutant Strains , Mice, SCID , NIMA-Interacting Peptidylprolyl Isomerase , Peptidylprolyl Isomerase/genetics , Pregnancy , S Phase/physiology , Tumor Suppressor Protein p53/genetics
2.
Eur J Immunol ; 35(3): 786-95, 2005 Mar.
Article in English | MEDLINE | ID: mdl-15719365

ABSTRACT

Signals that regulate T cell homeostasis are not fully understood. G protein-coupled receptors (GPCR), such as the chemokine receptors, may affect homeostasis by direct signaling or by guiding T cell migration to distinct location-restricted signals. Here, we show that blockade of Galphai-associated GPCR signaling by treatment with pertussis toxin led to T cell atrophy and shortened life-span in T cell-replete hosts and prevented T cell homeostatic growth and proliferation in T cell-deficient hosts. In vitro, however, neither GPCR inhibition nor chemokine stimulation affected T cell atrophy, survival, or proliferation. These findings suggest that GPCR signals are not trophic stimuli, but instead may be required for migration to distinct trophic signals, such as IL-7 or self-peptide/MHC. Surprisingly, while chemokines did not affect atrophy, atrophic T cells displayed increased chemokine-induced chemotaxis that was prevented by IL-7 and submitogenic anti-CD3 antibody treatment. This increase in migration was associated with increased levels of GTP-bound Rac and the ability to remodel actin. These data suggest a novel mechanism of T cell homeostasis wherein GPCR may promote T cell migration to distinct location-restricted homeostatic trophic cues for T cell survival and growth. Homeostatic trophic signals, in turn, may suppress chemokine sensitivity and cytoskeletal remodeling, to inhibit further migration.


Subject(s)
Chemotaxis/immunology , Growth Substances/immunology , Homeostasis/immunology , Receptors, G-Protein-Coupled/immunology , T-Lymphocytes/immunology , Actins/immunology , Actins/metabolism , Adoptive Transfer , Animals , Atrophy/immunology , Blotting, Western , Cell Proliferation/drug effects , Cell Survival/drug effects , Cytoskeleton/immunology , Cytoskeleton/metabolism , Flow Cytometry , Interleukin-7/immunology , Interleukin-7/metabolism , Lymphoid Tissue/pathology , Mice , Pertussis Toxin/pharmacology , Receptors, Antigen, T-Cell/immunology , Receptors, Antigen, T-Cell/metabolism , Receptors, Cytokine/immunology , T-Lymphocytes/drug effects
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