Cascading effects of insecticides and road salt on wetland communities.

Novel stressors introduced by human activities increasingly threaten freshwater ecosystems. The annual application of more than 2.3 billion kg of pesticide active ingredient and 22 billion kg of road salt has led to the contamination of temperate waterways. While pesticides and road salt are known to cause direct and indirect effects in aquatic communities, their possible interactive effects remain widely unknown. Using outdoor mesocosms, we created wetland communities consisting of zooplankton, phytoplankton, periphyton, and leopard frog (Rana pipiens) tadpoles. We evaluated the toxic effects of six broad-spectrum insecticides from three families (neonicotinoids: thiamethoxam, imidacloprid; organophosphates: chlorpyrifos, malathion; pyrethroids: cypermethrin, permethrin), as well as the potentially interactive effects of four of these insecticides with three concentrations of road salt (NaCl; 44, 160, 1600 Cl- mg/L). Organophosphate exposure decreased zooplankton abundance, elevated phytoplankton biomass, and reduced tadpole mass whereas exposure to neonicotinoids and pyrethroids decreased zooplankton abundance but had no significant effect on phytoplankton abundance or tadpole mass. While organophosphates decreased zooplankton abundance at all salt concentrations, effects on phytoplankton abundance and tadpole mass were dependent upon salt concentration. In contrast, while pyrethroids had no effects in the absence of salt, they decreased zooplankton and phytoplankton density under increased salt concentrations. Our results highlight the importance of multiple-stressor research under natural conditions. As human activities continue to imperil freshwater systems, it is vital to move beyond single-stressor experiments that exclude potentially interactive effects of chemical contaminants.

Salamander stress and duress: the relationship between CORT, autotomy and regeneration, and exploratory behaviour.

Responses to stress are generally mediated through the production of glucocorticoids by the hypothalamic-pituitary-adrenal (or -interrenal) axis. The prolonged production of stress hormones can contribute to delayed wound healing and growth, but little is known about their influence on regeneration following tail autotomy, or exploratory behaviour in autotomized individuals. Here we examined the relationship between stress, regeneration, and exploratory behaviour in Allegheny Mountain dusky salamanders (Desmognathus ochrophaeus) by manipulating corticosterone (CORT) levels via cutaneous patch. First, we measured tail regeneration in salamanders with elevated CORT for 13 weeks after the induction of tail autotomy. Test subjects received a weekly patch to wear for one hour that was saturated with either a low CORT (0.25 mg/ml) or high CORT (0.50 mg/ml) solution. Individuals receiving CORT patches regenerated significantly less of their tail length and volume (versus control), but without exhibiting dose-dependent effects. Second, we used a factorial design to evaluate the effects of autotomy and elevated CORT on exploration within a test arena consisting of low barriers arrayed in concentric rings. Individuals experiencing tail autotomy exhibited significantly less exploratory behaviour indicated by an increased latency to cross first barrier and a decreased number of barriers crossed. Neither elevated CORT (0.50 mg/ml), nor the interaction between elevated CORT and tail autotomy significantly affected salamander activity within the array. Although CORT did not have a direct effect on explorative behaviour, a delay in regeneration attributed to CORT could lead to changes in patterns of movement in autotomized individuals.