Environmental Conditions and Vehicle Disturbance Influence Stress Behaviors in a Working Harris's Hawk (Parabuteo unicinctus).

Harris's hawk (Parabuteo unicinctus) is used for pest control, as their presence can deter wild birds such as gulls. Working Harris's hawk on UK waste sites is permitted in accordance with regulations and legislation. This study investigated the general environment of a waste site compound yard where a single Harris's hawk was flown for pest control. The hawk's behaviors were evaluated in an ethogram, alongside environmental measures, and disturbance levels. Data was analyzed using Generalised Linear Latent Variable Models (GLLVM) to elucidate the effects of disturbance and environment on hawk behaviors. Results suggested cloudy conditions encouraged grooming responses that were normal and relaxed in their nature. Rain, sun and wind conditions increased recognized stress behaviors. Frequency of disturbance by construction vehicles inside the compound increased stress behaviors, such that keepers are recommended to revise welfare conditions. Increased stress behaviors by birds worked in dynamic environments like waste recycling yards could potentially elicit damaging illness such as feather breaking behavior. Reducing stress factors for Harris's hawk in industrial working yards combined with amending husbandry practices will improve welfare for the species.

Site-specific protein conjugates incorporating Para-Azido-L-Phenylalanine for cellular and in vivo imaging.

This work features the use of amber suppression-mediated unnatural amino acid (UAA) incorporation into proteins for various imaging purposes. The site-specific incorporation of the UAA, p-azido-L-phenylalanine (pAzF), provides an azide handle that can be used to complete the strain promoted azide-alkyne click cycloaddition (SPAAC) reaction to introduce an imaging modality such as a fluorophore or a positron emission tomography (PET) tracer on the protein of interest (POI). Such methodology can be pursued directly in mammalian cell lines or on proteins expressed in vitro, thereby conferring a homogeneous pool of protein conjugates. A general procedure for UAA incorporation to use with a site-specific protein labeling method is provided allowing for in vitro and in vivo imaging applications based on the representative proteins PTEN and PD-L1. This approach would help elucidate the cellular or in vivo biological activities of the POI.

Semi-syntheses and interrogation of indole-substituted Aspidosperma terpenoid alkaloids.

We demonstrated here a series of Aspidosperma terpenoid alkaloids can be quickly prepared using semisynthesis from naturally sourced tabersonine, featuring multiple oxygen-based substituents on the indole ring such as hydroxy and methoxy groups. This panel of complex compounds enabled the exploration of indole modifications to optimize the indole alkaloids' anticancer activity, generating lead compounds (e.g., with C15-hydroxy, C16-methoxy, and/or C17-methoxy derivatizations) that potently inhibit cancer cell line growth in the single-digit micromolar range. These results can help guide the development of Aspidosperma terpenoid alkaloid therapeutics. Furthermore, this synthetic approach features late-stage facile derivatization on complex natural product molecules, providing a versatile path to indole derivatization of this family of alkaloids with diverse chemical functionalities for future medicinal chemistry and chemical biology discoveries.

Concentration Dependence of a Hydrogel Phase Formed by the Deprotonation of the Imidazole Side Chain of Glycylhistidylglycine.

Upon deprotonation of its imidazole group at ∼pH 6, the unblocked tripeptide glycylhistidylglycine (GHG) self-assembles into very long crystalline fibrils on a 10-1000 µm scale which are capable of forming a volume spanning network, that is, hydrogel. The critical peptide concentration for self-assembly at a pH of 6 lies between 50 and 60 mM. The fraction of peptides that self-assemble into fibrils depends on the concentration of deprotonated GHG. While IR spectra seem to indicate the formation of fibrils with standard amyloid fibril ß-sheet structures, vibrational circular dichroism spectra show a strongly enhanced amide I' signal, suggesting that the formed fibrils exhibit significant chirality. The fibril chirality appears to be a function of peptide concentration. Rheological measurements reveal that the rate of gelation is concentration-dependent and that there is an optimum gel strength at intermediate peptide concentrations of ca. 175 mM. This paper outlines the unique properties of the GHG gel phase which is underlain by a surprisingly dense fibril network with an exceptionally strong modulus that make them potential additives for biomedical applications.

Exploring the gel phase of cationic glycylalanylglycine in ethanol/water. I. Rheology and microscopy studies.

HYPOTHESIS: The cationic tripeptide glycylalanylglycine (GAG) self-assembles into long, thick crystalline fibrils in an ethanol/water solution. At sufficiently high concentrations, the fibrils form a volume spanning hydrogel network. We report an extensive rheology and microscopy-based study regarding the self-assembly of GAG in ethanol/water solutions to understand the conditions for fibril formation as well as the thermal stability for future developments of this material. EXPERIMENTS: By systematically varying GAG concentration and ethanol fraction, we observe a two-dimensional fibril aggregate phase diagram. Microscopy studies shed light on the shape and size of fibrils as well as the macroscopic packing depending on conditions. The kinetics and evolution of the macroscopic fibril microstructure was investigated using rheology. FINDINGS: The mechanism of fibril formation is put into the context of a solubility framework, where ethanol reduces peptide solubility and induces self-assembly. The rate of fibril formation and strength of the gel can be controlled by peptide concentration and ethanol fraction. The faster rate of fibril formation leads to inhomogeneous packing of fibrils denoted by discrete dense fibril clusters. The solubility of the fibrils can be manipulated by temperature making the gel thermo-switchable, a property of interest for biomedical systems.

Climate structuring of Batrachochytrium dendrobatidis infection in the threatened amphibians of the northern Western Ghats, India.

Batrachochytrium dendrobatidis (Bd) is a pathogen killing amphibians worldwide. Its impact across much of Asia is poorly characterized. This study systematically surveyed amphibians for Bd across rocky plateaus in the northern section of the Western Ghats biodiversity hotspot, India, including the first surveys of the plateaus in the coastal region. These ecosystems offer an epidemiological model system since they are characterized by differing levels of connectivity, edaphic and climatic conditions, and anthropogenic stressors. One hundred and eighteen individuals of 21 species of Anura and Apoda on 13 plateaus ranging from 67 to 1179 m above sea level and 15.89 to 17.92° North latitude were sampled. Using qPCR protocols, 79% of species and 27% of individuals tested were positive for Bd. This is the first record of Bd in caecilians in India, the Critically Endangered Xanthophryne tigerina and Endangered Fejervarya cf. sahyadris. Mean site prevalence was 28.15%. Prevalence below the escarpment was 31.2% and 25.4% above. The intensity of infection (GE) showed the reverse pattern. Infection may be related to elevational temperature changes, thermal exclusion, inter-site connectivity and anthropogenic disturbance. Coastal plateaus may be thermal refuges from Bd. Infected amphibians represented a wide range of ecological traits posing interesting questions about transmission routes.

Micro-habitat distribution drives patch quality for sub-tropical rocky plateau amphibians in the northern Western Ghats, India.

The importance of patch quality for amphibians is frequently overlooked in distribution models. Here we demonstrate that it is highly important for the persistence of endemic and endangered amphibians found in the threatened and fragile ecosystems that are the rocky plateaus in Western Maharashtra, India. These plateaus are ferricretes of laterite and characterise the northern section of the Western Ghats/Sri Lanka Biodiversity Hotspot, the eighth most important global hotspot and one of the three most threatened by population growth. We present statistically supported habitat associations for endangered and data-deficient Indian amphibians, demonstrating significant relationships between individual species and their microhabitats. Data were collected during early monsoon across two seasons. Twenty-one amphibian taxa were identified from 14 lateritic plateaus between 67 and 1179m above sea level. Twelve of the study taxa had significant associations with microhabitats using a stepwise analysis of the AICc subroutine (distLM, Primer-e, v7). Generalist taxa were associated with increased numbers of microhabitat types. Non-significant associations are reported for the remaining 9 taxa. Microhabitat distribution was spatially structured and driven by climate and human activity. Woody plants were associated with 44% of high-elevation taxa. Of the 8 low-elevation taxa 63% related to water bodies and 60% of those were associated with pools. Rock size and abundance were important for 33% of high elevation specialists. Three of the 4 caecilians were associated with rocks in addition to soil and stream presence. We conclude the plateaus are individualistic patches whose habitat quality is defined by their microhabitats within climatic zones.

Description of male Tyloridasataraensis Kulkarni, 2014 (Araneae, Tetragnathidae) with notes on habits and conservation status.

The male sex of Tyloridasataraensis Kulkarni, 2014 is described based on specimens from the type locality. The distinguishing characters from its closest species Tyloridaventralis (Thorell, 1877) are detailed. An interesting behaviour of going underwater by T.sataraensis, on disturbance is recorded and tested for significance. The surveys have shown sighting of this species only to the perennial streams of the rocky outcrops in Satara region. The potential threats to this species and the possible conservation status based on known distribution are discussed.