ABSTRACT
The applicability of next generation DNA sequencing (NGS) methods for water quality assessment has so far not been broadly investigated. This study set out to evaluate the potential of an NGS-based approach in a complex catchment with importance for drinking water abstraction. In this multi-compartment investigation, total bacterial communities in water, faeces, soil, and sediment samples were investigated by 454 pyrosequencing of bacterial 16S rRNA gene amplicons to assess the capabilities of this NGS method for (i) the development and evaluation of environmental molecular diagnostics, (ii) direct screening of the bulk bacterial communities, and (iii) the detection of faecal pollution in water. Results indicate that NGS methods can highlight potential target populations for diagnostics and will prove useful for the evaluation of existing and the development of novel DNA-based detection methods in the field of water microbiology. The used approach allowed unveiling of dominant bacterial populations but failed to detect populations with low abundances such as faecal indicators in surface waters. In combination with metadata, NGS data will also allow the identification of drivers of bacterial community composition during water treatment and distribution, highlighting the power of this approach for monitoring of bacterial regrowth and contamination in technical systems.
Subject(s)
Bacteria/isolation & purification , DNA, Bacterial/genetics , Fresh Water/microbiology , High-Throughput Nucleotide Sequencing/methods , RNA, Ribosomal, 16S/genetics , Bacteria/classification , Bacteria/genetics , Feces/microbiology , Fresh Water/analysis , Water Pollution , Water QualityABSTRACT
The authors study 65 cases of repair of digital nerve lesions performed in 60 patients. They evaluate the quality of sensory recovery by clinical (Dellon and Weber Tests) and electrophysiological tests (velocity, amplitude and duration of the Compound Sensory Action Potential). The results show a complete recovery in 26%, a recovery of discrimination sensitivity in 73.8% and a recovery of protective sensation in 96.9% of the cases. Age and severity of the associated trauma are the most important factors influencing the quality of the sensory recovery.
