[Specific humoral response in Balb/c mice inoculated with a genome library of expression of Trypanosoma cruzi]. / Respuesta humoral específica en ratones Balb/c inoculados con una librería genómica de expresión de Trypanosoma cruzi.

A genomic expression library of Trypanosoma cruzi (T. cruzi) was made using plasmid pcDNA3 as a vector, with which male mice from the Balb/c isogenic line were intramuscullary inoculated. It was used a positive control group that was administered soluble antigens of T. cruzi. Other 2 groups received genomic and plasmid DNA, respectively. One group was not immunized. Weekly blood samples were obtained from all the animals until the fourth week and 2 weeks after reimmunization to study the response of specific antibodies against the microorganism antigens by an indirect immunoenzymatic assay (ELISA). It was observed a significant increase of specific antibodies in the animals reimmunized with 50 micrograms of the library, as well as in the group immunized with soluble antigens of T. cruzi.

[Intravenous streptokinase in acute myocardial infarction. A Mexican multicentric study]. / Estreptoquinasa intravenosa en el infarto agudo del miocardio. Estudio multicéntrico mexicano.

To evaluate the benefits of intravenous streptokinase (SQIV) in acute myocardial infarction (AMI), we joined a group of ten Mexican university hospitals, that were coordinated by the National Institute of Cardiology of Mexico. We included patients less than 70 years of age admitted to the hospital with less than 6 hours from the onset of chest pain during their first myocardial infarction. All patients had ST segment elevation of 1.5 mm or more, and none had contraindication for SQIV. They received 1.5 millions of SQIV in one hour. Reperfusion criteria included absence of pain, ST segment reduction and a rapid rise and fall of enzyme levels. Angiographic criterion for reperfusion was the permeability of the affected coronary vessel. Of 66 patients studied, 57 (86%) had clinical reperfusion; of the 24 available angiographic studies, 92% demonstrated reperfusion. Eight (12%) of the patients had minor complications and 7 (10%) had serious complications. There were 0 deaths. We concluded that SQIV is a useful therapeutic procedure, easy to perform in general hospitals.

Tratamiento de la hipertension arterial con el uso combinado de medicamentos. / Arterial hypertension treated with drug combinations

Se estudiaron durante 12 semanas un total de 20 enfermos con hipertension esencial, moderada o severa, de los cuales 17 no habian podido alcanzar una buena respuesta con otros medicamentos antihipertensivos. Al finalizar el tratamiento, 70% de ellos habia logrado la normalizacion de las cifras tensionales, y en el resto (30%) se obtuvo una respuesta antihipertensiva parcial. La tolerancia del clorhidrato de prazosin fue muy buena; en ningun enfermo hubo necesidad de suspender su administracion. El prazosin constituye una buena opcion para el manejo de la hipertension arterial, solo o en combinacion con otros medicamentos antihipertensivos