ABSTRACT
An investigation into the influence of UV irradiation on elastin hydrolysates in the presence of collagen was carried out using UV-Vis spectroscopy and spectrofluorometry. It was found that the absorbance of elastin hydrolysates in solution increased during irradiation more than the absorbance of the elastin/collagen blend. The fluorescence of elastin hydrolysates was observed at 305nm and at 380nm after excitation at 270nm. For the elastin/collagen mixture in solution, fluorescence spectrum shows only one maximum at 305nm. UV irradiation caused fluorescence fading at 305nm. For irradiated elastin the fluorescence at 305nm decreased faster than for the irradiated elastin/collagen mixture. The maximum of the fluorescence peak was shifted for elastin by 4nm, whereas for the elastin/collagen blends the shift was only 1-2nm. All the obtained results point out the ability of mixing elastin and collagen, and suggest that the elastin/collagen mixture in solution is less sensitive to UV irradiation than elastin hydrolysates alone.
Subject(s)
Collagen/radiation effects , Elastin/radiation effects , Ultraviolet Rays , Animals , Aorta/chemistry , Collagen/chemistry , Collagen/isolation & purification , Elastin/chemistry , Elastin/isolation & purification , Hydrolysis , Rats , Spectrophotometry , Swine , Tendons/chemistryABSTRACT
An investigation into the influence of UV irradiation on elastin hydrolysates dissolved in water was carried out using UV-Vis spectroscopy and spectrofluorometry. It was found that the absorption of elastin hydrolysates in solution increased during irradiation of the sample. For fluorescence of elastin hydrolysates we observed both, a decrease and increase of this value during irradiation of the sample. After UV irradiation of the elastin solution we observed a minor increase of overall absorption, most notably between 250 nm and 280 nm. Moreover, after UV irradiation a wide peak emerged between 290 nm and 310 nm with maximum at about 305 nm. The new peak suggests that new photoproducts are formed during UV irradiation of elastin hydrolysates. The fluorescence of elastin hydrolysates was observed at 305 nm and at 380 nm after excitation at 270 nm. UV irradiation caused fluorescence fading at 305 nm and 380 nm. After 30 min of irradiation a new broad weak band of fluorescence, attributable to new photoproducts, emerged in the UV wavelength region with emission maximum between 400 nm and 500 nm.
