ABSTRACT
Mycoplasma gallisepticum belongs to the class Mollicutes and induces severe chronic respiratory disease in chickens. It lacks the cell wall and contains a very small genome and, accordingly, a reduced set of regulatory proteins. It is assumed that one of the regulatory mechanisms in mycoplasmas may be the dynamics of the spatial organization of the chromosome. M. gallisepticum has only two known nucleoid-associated (NAP) histone-like proteins (Hup_1 and Hup_2). To search for new potential NAP that may play a role in the infection process, we isolated nucleoid fractions from M. gallisepticum cells before and after infection of HD3 chicken erythroblast cell line and performed a comparative proteomic analysis of these fractions. We identified several potential NAP that included the components of the terminal organelle and adhesion, VlhA antigen, NADH oxidase, and PykF pyruvate kinase.
Subject(s)
Mycoplasma Infections , Mycoplasma gallisepticum , Animals , Chickens , Mycoplasma gallisepticum/genetics , Mycoplasma gallisepticum/metabolism , Organelles , ProteomicsABSTRACT
AIM: The study aimed at showing actual information about vestibular migraine and presenting different features of vestibular migraine for prevention of diagnostic mistakes and choosing an effective treatment. MATERIAL AND METHODS: Fifty patients with vestibular migraine were diagnosed according to the International Classification of Headache Disorders 3rd edition and examined in a neurological department. RESULTS: Problems with outpatient diagnosis and treatment were found. Many patients were not diagnosed correctly and, therefore, did not receive effective management of vestibular migraine. All clinical features, information of disease history and different objective signs were important for differential diagnosis with other types of dizziness. Management according to guidelines for migraine treatment and recent research of vestibular migraine showed its efficiency for patients. CONCLUSION: Spreading of actual information about vestibular migraine is important due to its underdiagnosis. Knowledge of clinical features is helpful for diagnosis and differential diagnosis. Treatment approaches according to guidelines and last research should be used.
Subject(s)
Headache/diagnosis , Migraine Disorders/diagnosis , Vertigo/diagnosis , Adult , Aged , Diagnosis, Differential , Diagnostic Errors , Female , Headache/drug therapy , Humans , Male , Middle Aged , Migraine Disorders/drug therapy , Outpatients , Treatment Outcome , Vertigo/drug therapyABSTRACT
A thermolabile extracellular entero-cytolysin (EC) from Vibrio cholerae non-O1 was purified by ammonium sulphate fractionation, DE-52 cellulose ion exchange chromatography, gel-filtration on Ultrogel AcA-44 and high performance liquid chromatography on a Mono Q. The purified EC had a molecular weight of 63 kD and an isoelectric point of 6.2. It was not inactivated by cholesterol or 5,5'-dithio-bis(2-nitrobenzoic acid), nor activated by dithiothreitol. EC had no immunological cross-reactivity with cholera toxin. The EC caused fluid accumulation in the intestines of infant rabbits, death of mice by intravenous injection, and increased vascular permeability in the paw oedema test in mice. V. cholerae non-O1 EC lysed erythrocytes from various species and cultured cells (CHO, L-929, L-41, HEp-2, Vero, MDCK and BHK-21). In contrast to cholera toxin, EC caused crude destruction of target cells. The EC caused hemolysis by a colloid-osmotic mechanism due to the formation of hydrophilic pores of 1.8-2.0 nm diameter in the cell membrane. This EC also was able to open pores in lipid membranes. The induced channels were anion-selective and had a diameter of 1.8-2.0 nm.
Subject(s)
Cytotoxins/toxicity , Enterotoxins/toxicity , Vibrio cholerae/chemistry , Animals , Capillary Permeability/drug effects , Cell Line , Cell Membrane/drug effects , Chromatography, Liquid , Cytotoxins/chemistry , Cytotoxins/isolation & purification , Electrophoresis, Polyacrylamide Gel , Enterotoxins/chemistry , Enterotoxins/isolation & purification , Erythrocyte Membrane/drug effects , Hemolysis , Ion Channels/drug effects , Isoelectric Point , Lethal Dose 50 , Lipid Bilayers , Mice , Molecular Weight , Rabbits , Vibrio cholerae/pathogenicityABSTRACT
Affinity labeling of 40S subunits from human placenta with 4-(N-2-chloroethyl-N-methylamino)benzylmethyl-[32P]phosphoamide s of oligoribonucleotides pAUG and pAUGU3 was studied. Covalent attachment of these derivatives to 40S subunits within the complexes with 40S subunits, formed in the presence of Met-tRNAf.eIF-2.GTP, was detected. Both rRNA and ribosomal proteins were modified. Fragments of 18S rRNA, containing sites of the reagent attachment were identified: 1058-1164 for pAUG derivative and 976-1057--for pAUG and pAUGU3 ones. The data obtained allowed to conclude that the presence of the neighbouring codon at the A-site, regardless of the presence of the tRNA in it, affects significantly the arrangement of the trinucleotide template in the codon-anticodon interaction region. The large subunit does not cause significant alterations in the structural organization of the codon-anticodon interaction region.
Subject(s)
Oligoribonucleotides/chemistry , Placenta/ultrastructure , Ribosomes/metabolism , Affinity Labels , Anticodon , Blotting, Southern , Codon , DNA, Ribosomal/metabolism , Female , Humans , Nitrogen Mustard Compounds/chemistry , Organophosphorus Compounds/chemistry , Pregnancy , RNA, Transfer, Met/metabolism , Templates, GeneticABSTRACT
A new method for isolation and purification of Vibrio cholerae non-01 cytolysin has been elaborated. It includes the steps of concentration by ammonium sulphate, ion exchange chromatography on DE-52-cellulose, gel filtration via ultragel AsA-44 and chromatography on Mono Q. Cytolysin is shown to be a 60 kD and pI 6.2 protein. It is not inactivated by thiol-restoring agents and induces the production of precipitating antibodies. In contrast to choleragen the protein cytolytically affects erythrocytes and cells of the line L-929. It also possesses the lethal and oedematous effect and demonstrates the enteropathogenic effect on nursing rabbits.
Subject(s)
Cytotoxins/isolation & purification , Vibrio cholerae/metabolism , Animals , Chromatography, Ion Exchange , Cytotoxins/chemistry , Cytotoxins/toxicity , Electrophoresis, Polyacrylamide Gel , Immunodiffusion , Lethal Dose 50 , Mice , RabbitsABSTRACT
Using radioindicative methods, cerebral hemodynamics was studied in 62 children aged 3 to 15 years in the acute period of head injury. The diagnostic findings of focal intracranial lesions are presented. On the basis of the clinical-neurologic findings, the patients were divided into three groups depending on the degree of the severity of traumatic lesion to the brain. Patients with mild and moderately grave craniocerebral injury were shown to have a symmetric decrease in the blood flow intensity while severe traumas were associated with disorders of vascular passage of the preparation in one cerebral hemisphere in the presence of general reduction in the cerebral blood flow.
