ABSTRACT
The interactions between selenium (sodium selenite), anthracycline antibiotics daunorubicin (DNR), and major contractile protein cardiac myosin (CM) were investigated. The results showed that the binding force between selenium and CM was 100 times stronger than that of DNR and CM. There was no marked influence on fluorescence intensity of DNR-CM at selenium concentrations of up to 20 µM. The co-administration of selenium (0.5-10.0 µg Se/ml) together with DNR resulted in a significant reduction in mice cardiotoxicity. However, selenium at the dose of 50.0 or 100.0 µg Se/ml afforded no obvious protection. The data indicate that selenium in the form of sodium selenite at appropriate dosage (<10.0 µg Se/ml) diminish the cardiac toxicity of DNR, potentially allowing the use of DNR at higher dosages in clinical cancer chemotherapy.
Subject(s)
Cardiac Myosins/metabolism , Daunorubicin/metabolism , Sodium Selenite/metabolism , Animals , Antibiotics, Antineoplastic/chemistry , Antibiotics, Antineoplastic/metabolism , Antibiotics, Antineoplastic/toxicity , Binding, Competitive , Cardiomegaly/chemically induced , Cardiomegaly/mortality , Cardiomegaly/prevention & control , Daunorubicin/chemistry , Daunorubicin/toxicity , Dose-Response Relationship, Drug , Male , Mice , Molecular Structure , Protein Binding , Sodium Selenite/pharmacology , Spectrometry, Fluorescence , Survival RateABSTRACT
In this study, poly(amic acid)-modified biomass was prepared to improve the adsorption capacities for two cationic dyes, methylene blue and basic magenta. X-ray photoelectron spectroscopy and potentiometric titration demonstrated that a large number of imide, amine, and carboxyl groups were introduced on the biomass surface, and the concentrations of these functional groups were calculated to be 0.27, 1.08, and 1.08 mmol g(-1) by using the first derivative method. According to the Langmuir equation, the maximum uptake capacities (q(m)) for methylene blue and basic magenta were 680.3 and 353.4 mg g(-1), respectively, which were 13- and sevenfold than that obtained on the unmodified biomass. Adsorption kinetics study showed that the completion of the adsorption process needed only 40 min, which is faster than the common sorbent such as activated carbon and resin. Experimental results showed that pH and ionic strength had little effect on the capacity of the modified biomass, indicating that the modified biomass had good potential for practical use.
Subject(s)
Benzene Derivatives/metabolism , Biomass , Methylene Blue/isolation & purification , Polymers/metabolism , Rosaniline Dyes/isolation & purification , Saccharomyces cerevisiae/metabolism , Adsorption , Benzene Derivatives/chemistry , Biodegradation, Environmental , Hydrogen-Ion Concentration , Kinetics , Methylene Blue/chemistry , Osmolar Concentration , Photoelectron Spectroscopy , Polymers/chemistry , Potentiometry , Rosaniline Dyes/chemistry , Saccharomyces cerevisiae/ultrastructure , TemperatureABSTRACT
In this study, poly (methacrylic acid) modified biomass was prepared to improve the adsorption capacities for three dyes: methylene blue (MB), rhodamine B (RB) and basic magenta (BM). FTIR and potentiometric titration demonstrated that a large number of carboxyl groups were introduced on the biomass surface, and the concentration of the functional group was calculated to be 1.4 mmol g(-1) by using the first and second derivative method. According to the Langmuir equation, the maximum uptake capacities (q(m)) for MB, RB and BM were 869.6, 267.4 and 719.4 mg g(-1), which were 17-, 11- and 12-fold of that obtained on the unmodified biomass, respectively. Adsorption kinetics study showed that the completion of the adsorption process needed only 70 min, which is faster than that occur with the common sorbent such as activated carbon and resin. Temperature and ionic strength experiment showed that they both had effect on the adsorption capacity of the modified biomass. Good result was obtained when the modified biomass was used to treat dye wastewater.
