ABSTRACT
The aim of the present study was to explore the protective effect of small interfering RNA (siRNA) against nuclear factor κB (NF-κB) p65 on sepsis-induced acute lung injury (ALI) in mice. In total, 70 male Kunming mice were randomly divided into a healthy control group, a sepsis group, a specific interfering group and a scrambled control group (Sc), and the latter three groups were divided into post-operational 6 and 12 h subgroups, each of which consisted of 10 mice. The mice were administered with NF-κB siRNA, scrambled siRNA and normal saline via tail vein injection. Following 1 h, a mouse model of septic ALI was produced by cecal ligation and puncture (CLP) in the two siRNA groups and the sepsis control group. At 6 and 12 h postoperation, the experimental mice were sacrificed and the lung tissue samples were collected. Histopathological changes, wet/dry ratio of lung weight, NF-κB protein and NF-κB p65 mRNA levels, matrix metalloproteinase-9 (MMP-9) mRNA and protein activity were detected. Compared with the sepsis group and the Sc at the corresponding time, the expression levels of NF-κB p65 mRNA, the lung injury of experimental mice, the wet/dry ratio and the levels of MMP-9 mRNA and protein activity decreased, and significant differences were observed at 6 h post-operation (P<0.05). RNA interference against NF-κB p65 was able to decrease the expression of NF-κB and further inhibit the early phasic excessive inflammatory reaction in sepsis, which may alleviate ALI.
Subject(s)
Acute Lung Injury/pathology , Sepsis/pathology , Transcription Factor RelA/metabolism , Acute Lung Injury/etiology , Acute Lung Injury/metabolism , Animals , Cecum/injuries , Disease Models, Animal , HEK293 Cells , Humans , Interleukin-17/metabolism , Interleukin-6/metabolism , Male , Matrix Metalloproteinase 9/metabolism , Mice , NF-kappa B/metabolism , RNA Interference , RNA, Messenger/metabolism , RNA, Small Interfering/metabolism , Sepsis/etiology , Sepsis/metabolism , Transcription Factor RelA/antagonists & inhibitors , Transcription Factor RelA/genetics , Tumor Necrosis Factor-alpha/metabolismABSTRACT
Maize cytoplasmic male sterile (CMS) lines and their homotype fertile lines were cultivated on soil column to study the differences of their root traits at different growth stages after anthesis. The results indicated that CMS lines had stronger ability of dry matter accumulation in their aboveground part, their grain yield was significantly higher than that of homotype fertile lines (P < 0.05), and their root dry mass was higher. In addition, the ratio of their root to canopy had a similar variation trend as root dry mass. The root mass of CMS lines in 0-20 cm and 20-40 cm soil layers was higher than that of homotype fertile lines, and the superiority was more obvious in 40-80 cm soil layer. Compared with homotype fertile lines, CMS lines had significantly higher root activity in 0-20 cm and 20-40 cm soil layers (P < 0.05), and significantly higher root SOD activity and soluble protein content (P < 0.05) in 40-80 cm soil layer. The root activity and root SOD activity and soluble protein content of CMS lines were significantly higher than those of their homotype fertile lines (P < 0.05) at late growth stage. In conclusion, CMS lines had the characteristics of larger root quantity in deeper soil layers and higher root physiological activity at late growth stage, which could prolong the functional period of root and postpone the process of senescence, possibly being one of the important reasons of the high grain yield of CMS lines.
