ABSTRACT
OBJECTIVE: To investigate the effect of Rutin on the function and morphology of liver in type 1 diabetes mice induced by streptozotocin (STZ). METHODS: Type 1 diabetic mice model was established with the treatment of STZ by a 5 d intraperitoneal administration to male Kunming mice. Normal group had 12 mice without treatment of STZ,the mice with DM after STZ treated were randomly divided into DM group,low-dose (50 mg/kg) Rutin group and high-dose (100 mg/kg) Rutin group,each group had 12 mice. The mice in normal and DM group were given sodium carboxymethyl cellulose (10 mg/kg). Each mice was given above drugs by intragastric administration for 8 weeks. Postprandial random blood glucose was measured at 4 weeks and 8 weeks and the levels of alanine aminotransferase (ALT),aspartate aminotransferase (AST),lactate dehydrogenase (LDH),total protein (TP) and albumin (ALB) in serum were detected by automatic biochemical analyzer after 8 weeks. The morphology of liver was observed by HE and Masson staining. The ultrastructure of liver tissue was observed by electron microscope. RESULTS: After a continuous small-dose injection of STZ,the success rate of diabetes model mice were up to 98%. The blood glucose of the model group was significantly increased (P<0.01),and the levels of ALT,AST and LDH in serum were significantly higher,and TP and ALB were lower than those in normal group (P<0.05,P<0.01). Compared to the DM group,the levels of blood glucose were lower (P<0.05) at 4 weeks and 8 weeks,the contents of ALT,AST and LDH were significantly decreased,and TP and ALB were improved in both Rutin dose group. High-dose group performed more obvious (P<0.05,P<0.01). Morphological observation showed the tissue morphology of Rutin treatment group were improved obviously,and the effect was more significant in high-dose Rutin group. CONCLUSION: Rutin may improve the liver function and reduce the damage of liver tissue in STZ-induced type 1 diabetic mice.
