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Advanced fluorescence lifetime imaging algorithms for CMOS single-photon sensor based multi-focal multi-photon microscopy.
Li, D D-U; Poland, S; Coelho, S; Tyndall, D; Zhang, W; Richardson, J; Henderson, R K; Ameer-Beg, S M.
Annu Int Conf IEEE Eng Med Biol Soc ; 2013: 3036-9, 2013.
Article in English | MEDLINE | ID: mdl-24110367

ABSTRACT

We have developed a fast hardware friendly bi-exponential fluorescence lifetime algorithm suitable for 2D CMOS single-photon avalanche diode (SPAD) arrays. The performance of the proposed algorithm against other techniques is demonstrated on the data from a plant specimen (Convallaria) by using 0.13µm CMOS SPAD arrays mounted on a multi-beam multi-photon microscopy system.


Subject(s)
Convallaria/physiology , Fluorescence Resonance Energy Transfer/instrumentation , Microscopy, Fluorescence/instrumentation , Algorithms , Equipment Design , Fluorescence Resonance Energy Transfer/methods , Image Processing, Computer-Assisted/instrumentation , Image Processing, Computer-Assisted/methods , Light , Microscopy, Fluorescence/methods , Monte Carlo Method , Optical Imaging , Photons , Protein Interaction Mapping , Semiconductors , Signal Processing, Computer-Assisted
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