Missing microbes in infants and children in the COVID-19 pandemic: a study of 1,126 participants in Beijing, China.

The COVID-19 pandemic has caused many fatalities worldwide and continues to affect the health of the recovered patients in the form of long-COVID. In this study, we compared the gut microbiome of uninfected infants and children before the pandemic began (BEFORE cohort, n=906) to that of after the pandemic (AFTER cohort, n=220) to examine the potential impact of social distancing and life habit changes on infant/children gut microbiome. Based on 16S rRNA sequencing, we found a significant change in microbiome composition after the pandemic, with Bacteroides enterotype increasing to 35.45% from 30.46% before the pandemic. qPCR quantification indicated that the bacterial loads of seven keystone taxa decreased by 91.69%-19.58%. Quantitative microbiome profiling, used to enhance the resolution in detecting microbiome differences, revealed a greater explained variance of pandemic on microbiome compared to gender, as well as a significant decrease in bacterial loads in 15 of the 20 major genera. The random forest age-predictor indicated the gut microbiomes were less mature in the after-pandemic cohort than in the before-pandemic cohort in the children group (3-12 years old) and had features of a significantly younger age (average of 1.86 years). Lastly, body weight and height were significantly lower in the after-pandemic cohort than in the before-pandemic cohort in infants (<1 year of age), which was associated with a decrease in bacterial loads in the fecal microbiome.

Chronic exercise interventions for executive function in overweight children: a systematic review and meta-analysis.

Objectives: To systematically evaluate the effectiveness of chronic exercise in physical activity (PA) as an intervention for executive functions (EFs) in children. Methods: We conducted a systematic search in the following online databases: Web of Science, Cochrane Library, PubMed, Embase, and EBSCOhost. The timing is from database inception to July 2023, following PRISMA guidelines. Our inclusion criteria required studies reporting executive function (EF) levels in overweight children (age 0-18 years) before and after interventions. The Cochrane risk of bias tool assessed study bias, and Egger's test examined publication bias. Subgroup analyses considered three moderators: intervention duration, weekly frequency, and session length. Results: The meta-analysis included a total of 10 studies with 843 participants. It revealed a statistically significant yet relatively small overall positive effect (g = 0.3, 95% CI 0.16-0.44, P < 0.01) of chronic exercise on EF in overweight children. Importantly, there was no significant heterogeneity (Q = 11.64, df = 12, P = 0.48; I2 = 0). Conclusions: Chronic exercise interventions had a consistent positive impact on EF, irrespective of intervention duration, weekly frequency, or session length. However, given limitations in the number and design of studies, further high-quality research is needed to strengthen these conclusions. Systematic Review Registration: PROSPERO identifier (CRD42023468588).

Radiation polymerization for the preparation of universal coatings: remarkable anti-fogging and frost-resisting performance.

Hydrophilic anti-fogging coatings have attracted considerable attention due to their ease of preparation and excellent fog resistance. In this study, a hydrophilic anti-fogging coating based on the random copolymer p(AA-co-SAS) was prepared using acrylic acid (AA) and sodium allylsulfonate (SAS) as monomers through radiation polymerization. The introduction of SAS successfully transformed the random copolymer from a gel state into a film-forming polymer solution. The presence of AA structural units in p(AA-co-SAS) improved the film-forming properties of the polymer solution. Additionally, there was a positive correlation between the proportion of SAS structural units in the random copolymer and the scratch hardness and wetting properties of the coating. After coating polycarbonate (PC) sheets, the surface hydrophilicity was significantly enhanced, with the contact angle of PC-AA10/SAS5 decreasing from 100.1° to 18.8° within 50 seconds. The outstanding wetting properties endowed the coating with exceptional anti-fogging and frost-resisting performance. It exhibited optimal transparency under both testing conditions and demonstrated good stability during cyclic testing. Tape adhesion tests indicated that the adhesion between the coating and PC reached a 5B level. When AA10/SAS5 was applied to PET film, glass, and PMMA goggles, all samples showed excellent anti-fog performance. Even after being naturally placed for one year under ambient conditions, the PMMA goggles still maintained good performance in the anti-fog and frost resistance tests. The remarkable comprehensive properties of the polymer coating based on p(AA-co-SAS) suggest enormous potential applications in industries such as packaging, healthcare, and optical equipment.

Progress in Research on the Preparation of Graphene-Based Aerogels Using γ-ray Irradiation Technology.

Graphene aerogels (GAs) are of significant interest in the scientific community due to their unique attributes, including a three-dimensional porous structure, exceptional specific surface area, and remarkable chemical stability. Researchers have made notable breakthroughs in aerogel preparation, focusing on aspects like porous structures and chemical stability. This review explores product morphologies and properties developed between 2011 and 2023, particularly examining applications of graphene aerogels with amine or alcohol radical scavengers. It offers a roadmap for researchers, suggesting possibilities for radiation-based preparation and indicating broader applications. These findings contribute to a deeper understanding of aerogels and expand the potential applications of graphene aerogels across various fields.

Adverse Childhood Experiences and Depression among Homeless Young Adults: A Social Determinants of Health Perspective.

Homelessness is a pervasive issue in the United States that presents significant challenges to public health. Homeless young adults (HYAs) are at particular risk for increased incidence and severity of depression. Using primary survey data (n = 205) collected in the Phoenix Metropolitan Area, Arizona, from June to August 2022, this study aims to examine the relationship between adverse childhood experiences (ACEs) and depression among HYAs. We adopted the ACEs 10-item scale to measure childhood traumatic experiences, whereas depression was measured by using a PHQ-4 depression scale and diagnosed depression. Regression models were conducted to test the relationships between ACEs and depression outcomes while controlling for the covariates at the individual, interpersonal, and socioeconomic/living environment levels. The average PHQ-4 score was 5.01 (SD = 3.59), and 59.69% of HYAs reported being diagnosed previously with depression. The mean ACEs score was 5.22 out of 10. Other things being equal, for every one unit increase in ACEs scores, the odds of being diagnosed with depression increased by 11.5%, yet it was not statistically significant, while the PHQ-4 score increased by 0.445 (p < 0.001). Overall, HYAs were disproportionately affected by depression. This study elucidates the complex relationship between ACEs and depression among HYAs.

Analysis of the coupled and coordinated development of sports and tourism industries and the driving factors.

The Yellow River Basin has a wealth of tourism resources, a long history of folk sports, and strong legislative support, all of which are advantages for the growth of the sports tourism sector. This study constructs an evaluation index system based on panel data from nine provinces and regions in the Yellow River Basin from 2011 to 2020, and then measures the development index and analyzes the driving factors using the entropy method, coupling coordination degree model, kernel density estimation method, and grey correlation analysis method. The findings demonstrate that (1) the Yellow River Basin's overall level of growth in the sports and tourist sectors exhibits a consistent and upward trend and that Shandong and Henan provinces clearly have an edge over other provinces in terms of development. (2) The geographical development pattern was lower Yellow River > upper Yellow River > middle Yellow River, and the coupling coordination status shifted from slight disorder to good coordination. (3) There is a close connection between sports tourism and variables including population density, financial prowess, and infrastructure growth. The employment population index has the smallest link with invention patent authorization. The macro-policy framework should therefore be strengthened in the future, the sports tourism infrastructure should be improved, and the Yellow River basin's unique sports tourism resources should be fully utilized in order to increase brand influence. In addition, new sports tourism products should be developed in response to market demand and consumer preferences, and enterprise innovation and research and development efforts should be increased in order to achieve high-quality development transformation.

COVID-19 and primary wound healing: A new insights and advance.

With the outbreak and pandemic of coronavirus disease-2019 (COVID-19), a huge number of people died of it. Apart from lung injuries, multiple organs have been confirmed to be impaired. In COVID-19 time, primary wound healing processes always prolong, however, its possible underlying mechanisms are still unclear. Therefore, to overcome this clinical problem, clarifying its underlying mechanisms clearly is necessary and urgently needed. In this review, we summarized that COVID-19 can prolong primary wound healing by inducing excessive inflammation and oxidative stress, disturbing immune system and haematological system, as well as influencing the functions and viability of epidermal stem cells (ESCs). Otherwise, we summarized that the strict control measures of blocking up COVID-19 pandemic can also have side effects on primary wound healing process.

Green preparation of silver nanocluster composite AgNCs@CF-g-PAA and its application: 4-NP catalytic reduction and hydrogen production.

4-Nitrophenol (4-NP) is a serious organic environmental pollutant. Conversion of 4-nitrophenol to 4-aminophenol (4-AP) by catalytic hydrogenation is an effective solution. In this work, a catalyst (AgNCs@CF-g-PAA) loaded with silver nanoclusters (AgNCs) was prepared by radiation technique. Firstly, the template polyacrylic acid (PAA) was grafted onto the cotton fiber (CF) by radiation grafting technique to obtain a solid template (CF-g-PAA). After that, AgNCs were synthesized in situ on CF-g-PAA by radiation reduction, and the composite material AgNCs@CF-g-PAA was obtained directly. AgNCs@CF-g-PAA has an obvious photoluminescence phenomenon, which is attributed to the stable AgNCs binding to the carboxyl on the PAA molecular chain. Due to the extremely small size of AgNCs, AgNCs@CF-g-PAA has good catalytic characteristics. The prepared AgNCs@CF-g-PAA catalyst has a very high catalytic rate for the hydrogenation of 4-NP. Even at high concentrations of 4-NP, AgNCs@CF-g-PAA can still maintain a high catalytic rate. At the same time, the AgNCs@CF-g-PAA catalyst can also be used to catalyze the rapid hydrolysis of sodium borohydride, which is conducive to hydrogen production. In summary, we have prepared a practical catalyst AgNCs@CF-g-PAA with high catalytic performance based on cheap raw materials and a simple synthesis route, which provides a catalyst candidate for the treatment of water contaminant 4-NP and the production of hydrogen from sodium borohydride.

Role of curcumin in ischemia and reperfusion injury.

Ischemia-reperfusion injury (IRI) is an inevitable pathological process after organic transplantations. Although traditional treatments restore the blood supply of ischemic organs, the damage caused by IRI is always ignored. Therefore, the ideal and effective therapeutic strategy to mitigate IRI is warrented. Curcumin is a type of polyphenols, processing such properties as anti-oxidative stress, anti-inflammation and anti-apoptosis. However, although many researches have been confirmed that curcumin can exert great effects on the mitigation of IRI, there are still some controversies about its underlying mechanisms among these researches. Thus, this review is to summarize the protective role of curcumin against IRI as well as the controversies of current researches, so as to clarify its underlying mechanisms clearly and provide clinicians a novel idea of the therapy for IRI.

Research progress and insights on the role of ferroptosis in wound healing.

Ferroptosis is a newly discovered cell death type which is different from apoptosis, autophagy, pyroptosis as well as necrosis in the following aspects: morphology, biochemistry, gene and regulatory mechanisms. Ferroptosis is regulated by multiples of mechanisms such as system Xc- mechanism, glutathione peroxidase 4 (GPX4) mechanism, iron metabolism and lipid metabolism. Currently, ferroptosis has been revealed to be significant in wound healing such as diabetic wound, irradiated wound and ultraviolet (UV)-driven wound. Hence, how to intervene in the pathogenesis as well as the development of wounds and promote the wound healing by the regulation of ferroptosis have become a research hotspot. This review systematically summarises the latest scientific advances of ferroptosis and wound healing fields, with hoping to propose a new insight and advance in the wound treatment.

Choline Intake and Cognitive Function Among U.S. Older Adults.

Choline is an essential nutrient affects brain development in early life. However, evidence is lacking regarding its potential neuroprotective effects in later life from community-based cohorts. This study assessed the relationship between choline intake and cognitive functioning in a sample of older adults 60 years + from the National Health and Nutrition Examination Survey 2011-2012 and 2013-2014 waves (n = 2,796). Choline intake was assessed using two nonconsecutive 24-hour dietary recalls. Cognitive assessments included immediate and delayed word recalls, Animal Fluency, and Digit Symbol Substitution Test. The average daily dietary choline intake was 307.5 mg, and the total intake (including intake from dietary supplements) was 330.9 mg, both below the Adequate Intake level. Neither dietary OR = 0.94, 95% CI (0.75, 1.17) nor total choline intake OR = 0.87, 95% CI (0.70, 1.09) was associated with changes in cognitive test scores. Further investigation adopting longitudinal or experimental designs may shed light on the issue.

Two ambuic acid dimers derived from homo-endo Diels-Alder reaction from Pestalotiopsis versicolor.

Two new dimers of ambuic acid, pestaloversicoloric acids A (1) and B (2), and a known derivative, 13(S)-hydroxyambuic acid (3), were isolated from the static fermentation product of Pestalotiopsis versicolor. The structural identification was accomplished via analyses on the data of HR-MS, 1 D and 2 D NMR, and ECD. Different from the well-known exo-type dimer, torreyanic acid, compounds 1 and 2 represent the first example of endo-type product derived from the intermolecular Diels-Alder reaction of two molecules of ambuic acid derivative with the identical absolute stereochemistry.

Low Expression of Mitofusin 1 Gene Leads to Mitochondrial Dysfunction and Embryonic Genome Activation Failure in Ovine-Bovine Inter-Species Cloned Embryos.

Inter-species somatic cell nuclear transfer (iSCNT) is significant in the study of biological problems such as embryonic genome activation and the mitochondrial function of embryos. Here, we used iSCNT as a model to determine whether abnormal embryo genome activation was caused by mitochondrial dysfunction. First, we found the ovine-bovine iSCNT embryos were developmentally blocked at the 8-cell stage. The reactive oxygen species level, mitochondrial membrane potential, and ATP level in ovine-bovine cloned embryos were significantly different from both bovine-bovine and IVF 8-cell stage embryos. RNA sequencing and q-PCR analysis revealed that mitochondrial transport, mitochondrial translational initiation, mitochondrial large ribosomal subunit, and mitochondrial outer membrane genes were abnormally expressed in the ovine-bovine embryos, and the mitochondrial outer membrane and mitochondrial ribosome large subunit genes, mitochondrial fusion gene 1, and ATPase Na+/K+ transporting subunit beta 3 gene were expressed at lower levels in the ovine-bovine cloned embryos. Furthermore, we found that overexpression and knockdown of Mfn1 significantly affected mitochondrial fusion and subsequent biological functions such as production of ATP, mitochondrial membrane potential, reactive oxygen species and gene expressions in cloned embryos. These findings enhance our understanding of the mechanism by which the Mfn1 gene regulates embryonic development and embryonic genome activation events.

Determinants of School Diabetes Care Services and Quality Through the Lens of the School Nurse: A Qualitative Analysis.

Schools play a critical role in students' diabetes management and ensure their safety and well-being. We conducted key informant interviews with 11 school nurses in Missouri to assess determinants for diabetes care implementation. Five themes and 29 subthemes were identified concerning school nurses, schools, external stakeholders, government, and the COVID-19 pandemic. A social-ecological model was developed to elucidate each level's barriers, facilitators, and resources, and their interplay. School nurses should lead diabetes management, emergency planning, and health education for students/staff. Multiple gray areas existed regarding school nurses' specific roles/responsibilities. Lacking funding, insurance, and communication with parents/physicians further challenged diabetes care.

Deep learning with whole slide images can improve the prognostic risk stratification with stage III colorectal cancer.

BACKGROUND AND OBJECTIVE: Adjuvant chemotherapy is recommended as standard treatment for colorectal cancer (CRC) with stage III according to TNM stage. However, outcomes are varied even among patients receiving similar treatments. We aimed to develop a prognostic signature to stratify outcomes and benefit from different chemotherapy regimens by analyzing whole slide images (WSI) using deep learning. METHODS: We proposed an unsupervised deep learning network (variational autoencoder and generative adversarial network) in 180,819 image tiles from the training set (147 patients) to develop a WSI signature for predicting the disease-free survival (DFS) and overall survival (OS) of patients, and tested in validation set of 63 patients. An integrated nomogram was constructed to investigate the incremental value of deep learning signature (DLS) to TNM stage for individualized outcomes prediction. RESULTS: The DLS was associated with DFS and OS in both training and validation sets and proved to be an independent prognostic factor. Integrating the DLS and clinicopathologic factors showed better performance (C-index: DFS, 0.748; OS, 0.794; in the validation set) than TNM stage. In patients whose DLS and clinical risk levels were inconsistent, their risk of relapse was reclassified. In the subgroup of patients treated with 3 months, high-DLS was associated with worse DFS (hazard ratio: 3.622-7.728). CONCLUSIONS: The proposed based-WSI DLS improved risk stratification and could help identify patients with stage III CRC who may benefit from the prolonged duration of chemotherapy.

Social media-assisted interventions on human papillomavirus and vaccination-related knowledge, intention and behavior: a scoping review.

Social media holds the potential to engage adolescents and young adults and to facilitate interventions improving Human Papillomavirus Vaccine (HPVV). This article systematically reviewed the literature on Cochrane Library, PubMed, Web of Science, EMBASE, Scopus and CINAHL. Interventions delivered or facilitated by social media with outcomes of HPV-related knowledge, awareness, attitude, vaccination intention and behavior were included. Standardized forms were used to abstract the basic characteristics, settings, guiding theories and key findings of the interventions. Twenty-four studies met the eligibility criteria. Sixteen were educational interventions, and the other eight investigated the effect of social media message contents on improving Human Papillomavirus (HPV)-related outcomes. The studies were published between 2015 and 2021. The most frequently used social media platforms were Facebook, and the most commonly adopted theory was the health belief model (HBM). Existing interventions have shown preliminary but promising effects in improving HPV awareness and knowledge. Still, such improvements have not always been translated to improved behavioral intentions and vaccination rates. The contents and phrasing of social media messages and pre-existing individual characteristics of social media users moderated intervention effectiveness. Social media could be a valuable tool for engaging participants and delivering HPV interventions. Future interventions should apply stronger theory bases.

PDBL: Improving Histopathological Tissue Classification With Plug-and-Play Pyramidal Deep-Broad Learning.

Histopathological tissue classification is a simpler way to achieve semantic segmentation for the whole slide images, which can alleviate the requirement of pixel-level dense annotations. Existing works mostly leverage the popular CNN classification backbones in computer vision to achieve histopathological tissue classification. In this paper, we propose a super lightweight plug-and-play module, named Pyramidal Deep-Broad Learning (PDBL), for any well-trained classification backbone to improve the classification performance without a re-training burden. For each patch, we construct a multi-resolution image pyramid to obtain the pyramidal contextual information. For each level in the pyramid, we extract the multi-scale deep-broad features by our proposed Deep-Broad block (DB-block). We equip PDBL in three popular classification backbones, ShuffLeNetV2, EfficientNetb0, and ResNet50 to evaluate the effectiveness and efficiency of our proposed module on two datasets (Kather Multiclass Dataset and the LC25000 Dataset). Experimental results demonstrate the proposed PDBL can steadily improve the tissue-level classification performance for any CNN backbones, especially for the lightweight models when given a small among of training samples (less than 10%). It greatly saves the computational resources and annotation efforts. The source code is available at: https://github.com/linjiatai/PDBL.

Implementation of School Diabetes Care in the United States: A Scoping Review.

Diabetes management at school demands close collaboration of multiple stakeholders, including students with diabetes and parents, school nurses, teachers/staff, and local health care providers. This scoping review identified and synthesized evidence concerning factors that contributed to the quality and effectiveness of diabetes care implementation in U.S. K-12 schools. Forty-six studies met the eligibility criteria and were included. Five common factors emerged surrounding training and experiences, communications, parent engagement, resource allocations, and school environment. Complex interactions between multiple stakeholders jointly determined the quality of school diabetes care. A conceptual model was established to elucidate the complex interactions between multiple stakeholders and the relevant facilitators and barriers. Future research should improve sample representativeness, contrast school diabetes care practices to the national guidelines, and assess the impact of the social, economic, and political environment at federal, state, local/district levels on school diabetes care implementation.

Whole egg consumption and cognitive function among US older adults.

BACKGROUND: Eggs are an affordable food providing many shortfall nutrients with the potential to improve cognitive health. We assessed the relationship between whole egg consumption and cognitive functioning among a US nationally representative sample of older adults. METHODS: Individual-level data (2816 adults, aged ≥ 60 years) were obtained from the National Health and Nutrition Examination Survey (NHANES) 2011-2012 and 2013-2014 waves. Cognitive assessments included the Consortium to Establish a Registry for Alzheimer's Disease Word List Learning Test (CERAD-WL), Word List Recall Test (CERAD-DR), Animal Fluency Test (AF) and Digit Symbol Substitution Test (DSST). A composite cognitive z-score was constructed based on the individual tests to represent one's overall cognitive functioning. Multiple linear and logistic regressions were performed to examine the effect of whole egg consumption on cognitive functioning, adjusting for individual characteristics and survey design. RESULTS: Approximately 57% of older adults consumed whole eggs, with an average daily intake of 34 g of whole egg equivalent among consumers. The average z-scores of the CERAD-WL, CERAD-DR, AF and DSST tests were -0.08, 0.23, 0.37 and 0.42, respectively, and the overall composite cognitive z-score was 0.24 among older adults. Regression analyses found that neither whole egg consumption status, nor daily intake quantity was associated with cognitive test scores. CONCLUSIONS: No association was found between whole egg consumption and cognitive functioning among US older adults. Study limitations included cross-sectional study design and measurement errors. Future studies with longitudinal or experimental design are warranted to examine the possible link between egg consumption and cognition in older adults.