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1.
Huan Jing Ke Xue ; 34(4): 1298-307, 2013 Apr.
Article in Chinese | MEDLINE | ID: mdl-23798106

ABSTRACT

To understand riverine process of non-point source effectively, first flush effects of storm events were investigated at Baoxiang River of Lake Dianchi Watershed. Three sampling stations were selected along Baoxiang River for observing the flow rate and pollutant concentrations of the first three storm events from June 2009 to August 2009. Net discharged volume, net discharged loading, and net event mean concentration (EMC(n)) were proposed with their calculation methods. According to the analysis of three storm events at three stations, the following results colcd be extracted: (1) the larger the percent of impervious land and population density were, the higher EMC(n) of TSS, TN, TP, permanganate index and their cumulative curves [M(V)] were along the river; (2) TSS, TP loadings as well as their M (V) were positively correlated to the storm intensity, while TN and permanganate index loadings were consistent with the total rainfall of each storm event, where the percent of NO3(-) -N in total nitrogen decreased gradually when the number of storm events increased; (3) compared to tradition EMC, EMC(n) was proven to be a better indicator to accurately uncover and magnify the differences in first flush effects of storm events among different stations or storm events.


Subject(s)
Environmental Monitoring , Rain , Rivers , Water Movements , Water Pollutants, Chemical/analysis , China , Eutrophication , Lakes
2.
J Asian Nat Prod Res ; 15(3): 276-85, 2013.
Article in English | MEDLINE | ID: mdl-23421517

ABSTRACT

Multidrug resistance (MDR) has been a major problem in cancer chemotherapy. In this study, the aim was to explore the reversal effect and its potential mechanism of rosmarinic acid (RA) on SGC7901/Adr cells. 3-(4,5-Dimethylthiazol)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to investigate the reversal index of RA in SGC7901/Adr cell line. The intracellular accumulation of adriamycin, rhodamine123 (Rh123), and the expression of P-glycoprotein (P-gp) were assayed by flow cytometry. The influence of RA on the transcription of MDR1 gene was determined by reverse transcription-polymerase chain reaction. The results showed that RA could reverse the MDR of SGC7901/Adr cells, increase the intracellular accumulation of Adr and Rh123, and decrease the transcription of MDR1 gene and the expression of P-gp in SGC7901/Adr cells. These results indicated that RA was a potential multidrug resistance-reversing agent and warranted further investigations.


Subject(s)
Cinnamates/pharmacology , Depsides/pharmacology , Drug Resistance, Multiple/drug effects , ATP Binding Cassette Transporter, Subfamily B/pharmacology , ATP Binding Cassette Transporter, Subfamily B, Member 1/biosynthesis , ATP Binding Cassette Transporter, Subfamily B, Member 1/genetics , Doxorubicin/metabolism , Doxorubicin/pharmacology , Drug Resistance, Neoplasm/drug effects , Humans , Molecular Structure , Stomach Neoplasms/metabolism , Vincristine/pharmacology , Rosmarinic Acid
4.
Zhongguo Zhong Yao Za Zhi ; 30(2): 129-33, 2005 Jan.
Article in Chinese | MEDLINE | ID: mdl-15714818

ABSTRACT

OBJECTIVE: To study the interactions between Ligusticum chuanxiong Hort extract and cardiac muscle membrane receptors. METHOD: The cell membrane of rabbit cardiac muscle was fixed on silicon to make cell membrane stationary phase (CMSP), and then the interactions were studied by comparing the retention characteristics of the extracts from different solvents with those of the antagonists or activators corresponding to known receptors in cardiac muscle membrane, and by competition effect on the retention characteristics of extracts when adding the antagonists or activators into the mobile phase. RESULT: Water extract and ethanol extract both had retentions on CMSP; the retention characteristics of water extract could be affected when water extract was in competition with the antagonists for alpha receptor, and could not be affected when with the activator beta1 receptor. CONCLUSION: It is possible that some components in water extract may combine with alpha receptor and no component with beta1 receptor, and that some components in ethanol extract may combine with cardiac muscle cell membrane. The process between active components and receptors in vivo can be imitated through the interactions between drugs and CMSP. The method provides references for the resolution of two applications: to screen the active components from Chinese medicine, and to figure out the type of receptors involved.


Subject(s)
Drugs, Chinese Herbal/pharmacology , Ligusticum/chemistry , Myocytes, Cardiac/metabolism , Plants, Medicinal/chemistry , Receptors, Adrenergic, alpha/metabolism , Adrenergic alpha-Agonists/metabolism , Adrenergic alpha-Antagonists/metabolism , Adrenergic beta-Agonists/metabolism , Adrenergic beta-Antagonists/metabolism , Animals , Cell Membrane/metabolism , Chromatography, High Pressure Liquid/methods , Drugs, Chinese Herbal/isolation & purification , Female , Male , Myocytes, Cardiac/cytology , Protein Binding , Rabbits , Receptors, Adrenergic, beta/metabolism
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