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1.
Biomacromolecules ; 22(5): 2224-2232, 2021 05 10.
Article in English | MEDLINE | ID: mdl-33909978

ABSTRACT

Cationic glycopolymers with structures similar to those of typical poly(ionic liquid)s (PILs) were synthesized via the quaternization reaction of poly(4-vinyl pyridine) with halogen-functionalized d-mannose and tetraphenylethylene units. Such postpolymerization modification provided PILs with aggregation-induced emission effect as well as specific carbohydrate-protein recognition with lectins such as concanavalin A. The interactions between cationic glycopolymers and different microorganisms, including Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli, were used for the killing, imaging, and detection of bacteria. Besides, these sugar-containing PILs showed a relatively low hemolysis rate due to the presence of saccharide units, which may have potential application in the field of biomaterials.


Subject(s)
Ionic Liquids , Staphylococcus aureus , Lectins , Mannose
2.
Macromol Rapid Commun ; 34(19): 1542-6, 2013 Oct.
Article in English | MEDLINE | ID: mdl-24038760

ABSTRACT

Multivalent binding is a key for many critical biological processes and unique recognition and specificity in binding enables many of different glycans and proteins to work in a great harmony within the human body. In this study, the binding kinetics of synthetic glycopolypeptides to the dendritic cell lectin DC-SIGN and their inhibition potential for DC-SIGN interactions with the gp120 envelope glycoprotein of HIV-1 (gp120) are investigated.


Subject(s)
Dendritic Cells/metabolism , Glycopeptides/metabolism , HIV Envelope Protein gp120/metabolism , HIV-1/metabolism , Circular Dichroism , Dendritic Cells/immunology , Glycopeptides/chemical synthesis , Glycopeptides/chemistry , HIV Envelope Protein gp120/antagonists & inhibitors , Humans , Kinetics , Lectins/metabolism , Polymers/chemistry , Protein Binding , Protein Structure, Secondary , Surface Plasmon Resonance
3.
Biochem Biophys Res Commun ; 427(3): 450-5, 2012 Oct 26.
Article in English | MEDLINE | ID: mdl-22842463

ABSTRACT

Allergic asthma is a chronic inflammatory disease regulated by coordination of T-helper2 (Th2) type cytokines and inflammatory signal molecules. Silibinin is one of the main flavonoids produced by milk thistle, which is reported to inhibit the inflammatory response by suppressing the nuclear factor-kappa B (NF-κB) pathway. Because NF-κB activation plays a pivotal role in the pathogenesis of allergic inflammation, we have investigated the effect of silibinin on a mouse ovalbumin (OVA)-induced asthma model. Airway hyperresponsiveness, cytokines levels, and eosinophilic infiltration were analyzed in bronchoalveolar lavage fluid and lung tissue. Pretreatment of silibinin significantly inhibited airway inflammatory cell recruitment and peribronchiolar inflammation and reduced the production of various cytokines in bronchoalveolar fluid. In addition, silibinin prevented the development of airway hyperresponsiveness and attenuated the OVA challenge-induced NF-κB activation. These findings indicate that silibinin protects against OVA-induced airway inflammation, at least in part via downregulation of NF-κB activity. Our data support the utility of silibinin as a potential medicine for the treatment of asthma.


Subject(s)
Antioxidants/therapeutic use , Asthma/drug therapy , Pneumonia/drug therapy , Silybum marianum , Silymarin/therapeutic use , Animals , Asthma/pathology , Bronchoalveolar Lavage Fluid/immunology , Cytokines/antagonists & inhibitors , Cytokines/biosynthesis , Female , Mice , Mice, Inbred BALB C , Pneumonia/pathology , Silybin , Th2 Cells/immunology
4.
Chem Commun (Camb) ; 48(65): 8063-5, 2012 Aug 21.
Article in English | MEDLINE | ID: mdl-22767077

ABSTRACT

The synthesis of cyclodextrin-centred star polymers via thiol-ene addition of per-6-thio-ß-cyclodextrin (CD-(SH)(7)) with vinyl terminated polymers is described. The obtained thiol-ene product was employed as an initiator for ring opening polymerization (ROP) of ε-caprolactone (ε-CL).


Subject(s)
Cellulose/chemical synthesis , Cyclodextrins/chemical synthesis , Polymerization , Sulfhydryl Compounds/chemistry , Caproates/chemical synthesis , Caproates/chemistry , Cellulose/chemistry , Cyclodextrins/chemistry , Lactones/chemical synthesis , Lactones/chemistry , Sulfhydryl Compounds/chemical synthesis , beta-Cyclodextrins/chemical synthesis , beta-Cyclodextrins/chemistry
5.
Yao Xue Xue Bao ; 47(1): 34-8, 2012 Jan.
Article in Chinese | MEDLINE | ID: mdl-22493802

ABSTRACT

This study is to investigate the anti-allergic effect of anthocyanidin and to explore its possible mechanism. The experiments of passive cutaneous anaphylaxis reaction (PCA) and colorimetry were used to determine the effect of anthocyanidin on degranulation of mast cells in vivo. For in vitro study, various concentrations of anthocyanidin (100, 50 and 25 micromol x L(-1)) were added to the culture medium of mast cells cultured with 100 microg x L(-1) of dinitrophenyl (DNP) specific IgE overnight. The azelastine (100 micromol x L(-1)) was selected as the positive control. The antigen (DNP-human serum albumin, DNP-HAS)-induced release of degranulation was measured by enzymatic assay, histamine was determined by EIA, and interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) were measured by Western blotting, separately. In addition, the effects of anthocyanidin on phosphorylation of NF-kappaB, p38MAPK and Akt were observed by Western blotting. The results showed that treatments with anthocyanidin (100 and 50 mg x kg(-1)) were followed by a decrease in PCA of rats. Anthocyanidin (100 and 50 micromol x L(-1)) obviously suppressed the degranulation from mast cells, whereas results from anthocyanidin (100 and 50 micromol x L(-1)) group indicated significant inhibitory effect on histamine, the calcium uptake, TNF-alpha, IL-6, phosphorylation of NF-kappaB, p38MAPK and Akt of mast cells induced by antigen. Anthocyanidin may suppress the anaphylactic reaction by inhibiting the action of mast cells. NF-kappaB, p38MAPK and Akt at least in part contribute to this event.


Subject(s)
Anthocyanins/pharmacology , Anti-Allergic Agents/pharmacology , Mast Cells/metabolism , Passive Cutaneous Anaphylaxis/drug effects , Animals , Calcium/metabolism , Cell Degranulation/drug effects , Histamine Release/drug effects , Immunoglobulin E/immunology , Interleukin-6/metabolism , Male , Mast Cells/immunology , Mast Cells/physiology , Proto-Oncogene Proteins c-akt/metabolism , Random Allocation , Rats , Rats, Sprague-Dawley , Signal Transduction , Transcription Factor RelA/metabolism , Tumor Necrosis Factor-alpha/metabolism , p38 Mitogen-Activated Protein Kinases/metabolism
6.
Biol Pharm Bull ; 34(7): 959-66, 2011.
Article in English | MEDLINE | ID: mdl-21719998

ABSTRACT

Cornuside, a secoiridoid glucoside compound, was isolated from the fruit of Cornus officinalis SIEB. et ZUCC. Cornuside has been reported to possess immunomodulatory and anti-inflammatory activities. However, the effects and mechanism of action of cornuside in inflammation have not been fully characterized. The present study was therefore designed to examine whether cornuside suppresses inflammatory response in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. Cornuside significantly inhibited the LPS-induced production of nitric oxide, prostaglandin E(2), tumor necrosis factor-alpha, interleukin-6 (IL-6), and IL-1beta. The mRNA and protein expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were also decreased by cornuside. Furthermore, cornuside significantly attenuated the LPS-stimulated phosphorylation and degradation of inhibitory kappa B-alpha and the subsequent translocation of the p65 subunit of nuclear factor-kappa B (NF-κB) to the nucleus. Cornuside also reduced the phosphorylations of extracellular-signal-related kinase (ERK1/2), p38, and c-Jun N-terminal kinase (JNK1/2). These results suggest that the anti-inflammatory property of cornuside is related to the downregulations of iNOS and COX-2 due to NF-κB inhibition as well as the negative regulation of ERK1/2, p38, and JNK1/2 phosphorylations in RAW 264.7 cells.


Subject(s)
Glucosides/pharmacology , Lipopolysaccharides/antagonists & inhibitors , Macrophages/drug effects , NF-kappa B/antagonists & inhibitors , Pyrans/pharmacology , Animals , Base Sequence , Cell Line , Cyclooxygenase 2/genetics , Cyclooxygenase 2/metabolism , Cytokines/antagonists & inhibitors , Cytokines/biosynthesis , DNA Primers , Dinoprostone/antagonists & inhibitors , Dinoprostone/biosynthesis , Lipopolysaccharides/pharmacology , Macrophages/enzymology , Macrophages/metabolism , Mice , NF-kappa B/metabolism , Nitric Oxide/antagonists & inhibitors , Nitric Oxide/biosynthesis , Nitric Oxide Synthase Type II/genetics , Nitric Oxide Synthase Type II/metabolism , Phosphorylation , Polymerase Chain Reaction , Protein Kinases/metabolism , RNA, Messenger/genetics
7.
Neurosci Lett ; 498(2): 147-52, 2011 Jul 08.
Article in English | MEDLINE | ID: mdl-21596098

ABSTRACT

The janus kinase/signal transducer and activator of transcription (JAK/STAT) is one of the main pathways downstream of cytokine receptors and growth factor receptors by transducing signals from cell surface to the nucleus. In this study, we aimed to survey the role of JAK2/STAT pathway in the progress of TBI. Right parietal cortical contusion in rats was induced by the Feeney free falling model. The activation of JAK2, STAT1 and STAT3 in pericontusional cortex was determined by Western blotting, electrophoretic mobility shift assay (EMSA), immunohistochemistry and immunofluorescence. Moreover, we assessed the neurological recovery (using Neurological Severity Scores (NSS)) of rats under the pretreatment of a JAK2 inhibitor, AG490. Western blotting revealed that expression of p-JAK2, p-STAT1 and p-STAT3 increased immediately, peaked at 3h after TBI and decreased thereafter, and the activation could be inhibited by AG490. Immunohistochemical study showed that JAK2/STAT pathway was activated in both neurons and astrocytes at 3h after TBI. STAT3-specific binding activity was obviously enhanced after TBI and down-regulated after AG490 administration. The higher NSS of TBI+AG490 group revealed a worse behavior recovery when compared with TBI+DMSO group. Our results suggest that the JAK2/STAT pathway is activated in pericontusional cortex of rats, and may be involved in the neurological function recovery after TBI.


Subject(s)
Brain Injuries/metabolism , Janus Kinase 2/physiology , Parietal Lobe/injuries , STAT1 Transcription Factor/physiology , STAT3 Transcription Factor/physiology , Signal Transduction/physiology , Animals , Astrocytes/metabolism , Brain Injuries/genetics , Contusions/genetics , Contusions/metabolism , Contusions/pathology , Enzyme Activation , Enzyme Induction , Gene Expression Regulation , Janus Kinase 2/antagonists & inhibitors , Janus Kinase 2/biosynthesis , Janus Kinase 2/genetics , Male , Neurons/metabolism , Parietal Lobe/metabolism , Parietal Lobe/pathology , Rats , Rats, Wistar , Recovery of Function , STAT1 Transcription Factor/biosynthesis , STAT1 Transcription Factor/genetics , STAT3 Transcription Factor/biosynthesis , STAT3 Transcription Factor/genetics , Time Factors , Tyrphostins/pharmacology , Tyrphostins/toxicity
8.
Brain Res ; 1396: 96-104, 2011 Jun 17.
Article in English | MEDLINE | ID: mdl-21530945

ABSTRACT

A growing body of evidence indicates that Toll-like receptors (TLRs) and Interleukin-1 (IL-1) family have been shown to be involved in the damaging inflammatory processes associated with stroke, infection, neoplasia, and other diseases in the central nervous system. Myeloid differentiation primary response protein 88 (Myd88) is a critical adaptor protein that transmits signals for TLRs and IL-1 family. Therefore, this study aimed to detect the expression of Myd88 protein and mRNA in a rat weight-dropping trauma model and to clarify the role of Myd88 after traumatic brain injury (TBI). A total of fifty-four Sprague Dawley (SD) rats were randomly divided into control group and TBI groups at hours 6, 12 and on day 1, day 2, day 3, and day 7. The TBI groups suffered experimental TBI by improved Feeney model. Myd88 expression is measured by Reverse Transcription PCR (RT-PCR), Western blot analysis and immunohistochemistry; and nuclear factor-kappaB (NF-κB) binding activity by electrophoretic mobility shift assay (EMSA); The levels of tumor necrosis factor-α (TNF-α) and Interleukin 1ß (IL-1ß) were measured by enzyme linked immunosorbent assay (ELISA) and the intercellular adhesion molecule-1 (ICAM-1) expression by immunohistochemistry. The expression of Myd88 in the injured brain was dramatically increased through 6 h and 7 days postinjury, and peaked on 3days. NF-κB, TNF-α, IL-1ß and ICAM-1 also ascended significantly after TBI. Our data demonstrated that Myd88 was increasingly expressed in a parallel time course to the up-regulation of NF-κB, proinflammatory cytokines and ICAM-1 and there was a highly positive relationship among them. These findings might have important implications during the administration of specific Myd88 antagonists in order to prevent or reduce inflammatory response after TBI.


Subject(s)
Brain Injuries/metabolism , Cerebral Cortex/injuries , Cerebral Cortex/metabolism , Myeloid Differentiation Factor 88/genetics , Animals , Brain Injuries/pathology , Cerebral Cortex/pathology , Cytokines/biosynthesis , Disease Models, Animal , Inflammation Mediators/metabolism , Intercellular Adhesion Molecule-1/metabolism , Interleukin-1beta/metabolism , Male , Myeloid Differentiation Factor 88/biosynthesis , NF-kappa B/biosynthesis , Random Allocation , Rats , Rats, Sprague-Dawley , Tumor Necrosis Factor-alpha/metabolism , Up-Regulation/drug effects , Up-Regulation/physiology
9.
Biosci Biotechnol Biochem ; 75(4): 656-61, 2011.
Article in English | MEDLINE | ID: mdl-21512227

ABSTRACT

This study elucidated the effects of cornuside on carbon tetrachloride (CCl4)-induced hepatotoxicity. Rats were treated intraperitoneally with 0.5 mL/kg of CCl4. Sixteen h after CCl4 treatment, the levels of serum aminotransferases, tumor necrosis factor-α (TNF-α), and lipid peroxidation were significantly elevated, whereas the hepatic antioxidative enzyme activities were decreased. These changes were attenuated by cornuside. Histological studies also indicated that cornuside inhibited CCl4-induced liver damage. Furthermore, the contents of hepatic nitrite, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) were elevated after CCl4 treatment, while cytochrome P450 2E1 (CYP2E1) expression was suppressed. Cornuside treatment inhibited the formation of liver nitrite, and reduced the overexpression of iNOS and COX-2 proteins, but restored the liver CYP2E1 content as compared with the CCl4-treated rats. Our data indicate that cornuside protects the liver from CCl4-induced acute hepatotoxicity, perhaps due to its ability to restore the CYP2E1 function and suppress inflammatory responses, in combination with its capacity to reduce oxidative stress.


Subject(s)
Carbon Tetrachloride/toxicity , Chemical and Drug Induced Liver Injury/prevention & control , Cytoprotection/drug effects , Glucosides/pharmacology , Pyrans/pharmacology , Animals , Antioxidants/metabolism , Chemical and Drug Induced Liver Injury/blood , Chemical and Drug Induced Liver Injury/metabolism , Chemical and Drug Induced Liver Injury/pathology , Cyclooxygenase 2/metabolism , Cytochrome P-450 CYP2E1/metabolism , Gene Expression Regulation, Enzymologic/drug effects , Lipid Peroxidation/drug effects , Liver/drug effects , Liver/enzymology , Liver/metabolism , Liver/pathology , Male , Microsomes, Liver/drug effects , Microsomes, Liver/metabolism , Nitric Oxide Synthase Type II/metabolism , Nitrites/metabolism , Rats , Rats, Sprague-Dawley , Transaminases/blood , Tumor Necrosis Factor-alpha/blood
10.
Chin J Traumatol ; 13(5): 293-6, 2010 Oct 01.
Article in English | MEDLINE | ID: mdl-20880456

ABSTRACT

OBJECTIVE: To assess the preventive effect of sodium valproate on early posttraumatic seizures in traumatic brain injury (TBI) patients. METHODS: The retrospective study was based on 159 patients with TBI treated at Department of Neurosurgery, Nanjing General Hospital of Nanjing Command enrolled between January 1, 2008 and December 31, 2009. The in-hospital section of the retrospectively collected database includes information on age, sex, initial Glasgow Coma Score (GCS), results of CT scanning, operation, usage of sodium valproate, seizures in the first week after injury and outcome. RESULTS: Seven patients (4.4%) showed early posttraumatic seizures. Although the incidence was zero in patients who received sodium valproate treatment, the difference between the treatment and control groups was not statistically significant. Of the 87 severe TBI patients (GCS 3-8), 6 patients in the control group (6.9%) suffered from early seizures during the first week after TBI and no patient who received preventive therapy suffered from seizures. The difference between the treatment and the control groups was still not statistically significant. Of the 72 mild and moderate TBI patients (GCS 9-15), only 1 patient in the control group suffered from seizures and no patient in the treatment group suffered. CONCLUSIONS: Although the results suggest that the study is not sufficiently powerful to detect a clinically important difference in the seizure rates between the treatment and control groups, sodium valproate is effective in decreasing the risk of early posttraumatic seizures in severe TBI patients. Further prospective studies are recommended.


Subject(s)
Anticonvulsants/therapeutic use , Brain Injuries/complications , Epilepsy, Post-Traumatic/prevention & control , Valproic Acid/therapeutic use , Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Retrospective Studies
11.
Dongwuxue Yanjiu ; 31(3): 239-49, 2010 Jun.
Article in Chinese | MEDLINE | ID: mdl-20672411

ABSTRACT

In order to study the structural, functional and molecular evolutional relationship of fish liportein lipase (LPL) and hepatic lipase (HL) genes, seven liver LPL and HL cDNA partial sequences were isolated from Acipenser sinensis, Hypophthalmichthys molitrix, Aristichthys nobilis, Ctenopharyngodon idellus, Cirrhinus molitorella, Oreochromis niloticus, Channa maculate by RT-PCR. Three full-length cDNA sequences of LPL, HL of Acipenser sinensis and LPL of Hypophthalmichthys molitrix were obtained by RACEs. From the sequence analysis and homologous results, the amino acid sequences of LPL and HL are relatively conserved in mammals, birds and fishes. Taken together with these obtained amino acid sequences and sequences of all known LPL, HL, EL and PL from other vertebrates, a phylogenetic tree was constructed by neighbor-joining method. The result supports that all of them belong to lipase family.


Subject(s)
Cricetinae/genetics , Cricetulus/genetics , DNA, Complementary/analysis , Evolution, Molecular , Fishes/genetics , Lipase/genetics , Amino Acid Sequence , Animals , Base Sequence , Carps , Cricetinae/classification , DNA, Complementary/genetics , Fishes/classification , Fresh Water , Lipoprotein Lipase/analysis , Lipoprotein Lipase/genetics , Molecular Sequence Data , Reverse Transcriptase Polymerase Chain Reaction
12.
BMB Rep ; 41(3): 204-9, 2008 Mar 31.
Article in English | MEDLINE | ID: mdl-18377723

ABSTRACT

The cDNAs encoding glutathione peroxidase (GPx) were cloned and sequenced from the liver of three Chinese carps with different tolerance to hepatotoxic microcystins, phytoplanktivorous silver carp (Hypophthalmichthys molitrix) and bighead carp (Aristichthys nobilis), and herbivorous grass carp (Ctenopharyngodon idellus). Using genome walker method, a 750 bp 5'-flanking region of the silver carp GPx gene was obtained, and several potential regulatory elements were identified in the promoter region of the GPx gene. The silver carp GPx gene was widely expressed in all tissues examined. Despite phylogenetic analysis, assigning this newly described carp GPx to the group of mammalian GPx2, the carp GPx seems more similar to GPx1 from a physiological point of view. The constitutive expression pattern of the three carp liver GPx gene, shows a positive relationship with their tolerance to microcystins.


Subject(s)
Carps/genetics , Glutathione Peroxidase/genetics , Liver/enzymology , 5' Flanking Region/genetics , Amino Acid Sequence , Animals , Base Sequence , Carps/classification , China , Cloning, Molecular , Gene Expression Profiling , Gene Expression Regulation, Enzymologic , Glutathione Peroxidase/chemistry , Glutathione Peroxidase/metabolism , Molecular Sequence Data , Phylogeny , RNA, Messenger/genetics , RNA, Messenger/metabolism , Sequence Analysis, DNA , Sequence Homology, Amino Acid
13.
Article in English | MEDLINE | ID: mdl-17537656

ABSTRACT

The full-length neuropeptide Y (NPY) cDNA of Chinese perch Siniperca chuatsi was 704 bp in length, and contained a 300 bp open reading frame encoding a prepro-NPY with 99 amino acids. The predicted prepro-NPY peptide contained a putative signal peptide of 28 amino acids and a mature NPY of 36 amino acids, followed by the proteolytic processing site Gly-Lys-Arg and 35 amino acids that comprise the C-terminal peptide of NPY. Amino acid alignment and phylogenetic analysis indicate that the predicted Chinese perch prepro-NPY (composed of 99 amino acids) had high identities to the prepro-NPY of acanthomorph fishes (93-95%), whereas it had much lower identities to the prepro-NPY (composed of 96 or 97 amino acids) of cyprinid fishes (59-60%) or mammals (57-63%). Chinese perch NPY gene consists of four exons and three introns. The ratio of intron 2 to intron 3 was over 14 in Chinese perch NPY gene, whereas this ratio was below 4 in zebrafish and mammalian NPY gene. The total size of the Chinese perch NPY gene was 2223 bp, which was only about 28% of the size of NPY gene in higher vertebrate. Analysis of a 1622 bp promoter region of Chinese perch NPY gene, revealed a typical TATA box, a GC box and an untypical CAAT box, located at 84 bp, 101 bp and 303 bp upstream of the start codon respectively. Three STAT binding site-like elements (TCCAGTA) which were necessary for the leptin-induced transcriptional control of rat NPY gene were identified. In consistence to the effect of cortisol on fish brain NPY mRNA expression, four glucocorticoid-responsive elements were detected. Besides the highest expression in brain, substantial level of Chinese perch NPY mRNA expression was detected in the spleen and liver, and trace level of NPY mRNA expression was also detected in the adipose tissue, intestine and muscle. These results indicated that Chinese perch NPY might be involved in the food intake control by leptin and cortisol system, and diversification of NPY signaling should exist between acanthomorph fishes and cyprinid fishes as well as mammals.


Subject(s)
Gene Expression Regulation , Neuropeptide Y/genetics , Amino Acid Sequence , Animals , Base Sequence , Brain/drug effects , Brain/metabolism , Cloning, Molecular , Female , Fishes , Gene Expression Regulation/drug effects , Hydrocortisone/pharmacology , Male , Molecular Sequence Data , Phylogeny , Polymerase Chain Reaction , RNA, Messenger/biosynthesis , Sequence Homology, Amino Acid
14.
Acta Crystallogr Sect E Struct Rep Online ; 64(Pt 1): m52, 2007 Dec 06.
Article in English | MEDLINE | ID: mdl-21200624

ABSTRACT

In the title compound, [Cu(C(7)H(3)Br(2)O(2))(2)], the Cu(II) atom, which lies on an inversion centre, is coordinated by four O atoms from two chelating bidentate 2,4-dibromo-6-formyl-phenolate ligands in a slightly distorted square-planar coordination geometry. In the crystal structure, short inter-molecular Br⋯Br [3.516 (4) and 3.653 (4) Å] and Cu⋯Br [3.255 (1) Å] contacts together with C-H⋯O hydrogen bonds generate a three-dimensional network.

15.
Zhonghua Wei Chang Wai Ke Za Zhi ; 9(4): 342-4, 2006 Jul.
Article in Chinese | MEDLINE | ID: mdl-16886120

ABSTRACT

OBJECTIVE: To explore the relationship between the changes of trace elements and lymphatic metastasis in gastric carcinoma. METHODS: Trace elements including Fe, Mg, Mn, Ca, Cu, Zn, Se were measured in primary gastric carcinoma and regional lymph nodes from 40 patients with gastric carcinoma, and compared among the primary tumor, metastatic, and non-metastatic nodes. RESULTS: There were no significant differences in the contents of Fe, Mg, Mn and Ca among primary gastric tumors, regional lymph nodes with or without metastasis (P=0.372 - 0.741, P > 005), and no significant differences in the contents of all 7 trace elements between primary tumors and metastatic lymph nodes (P=0.15 - 0.59, P > 005). Compared with metastatic lymph nodes, the contents of Zn, Se significantly decreased, while Cu and Cu/Zn significantly increased (P=0.001 - 0.009, P< 0.01) in non-metastatic lymph nodes. The content of Zn in N2 positive lymph nodes was significant lower than that in N1 positive nodes (P=0.027). There were no significant difference in the contents of all 7 elements between intestinal type and diffuse type (P=0.149 - 0.758, P > 0.05). CONCLUSIONS: Lymphatic metastasis of gastric cancer is concomitant with the changes of trace elements, and the changes of Zn, Cu, Se may be related with lymphatic metastasis.


Subject(s)
Lymph Nodes/metabolism , Stomach Neoplasms/metabolism , Stomach Neoplasms/pathology , Trace Elements/metabolism , Adult , Aged , Female , Humans , Lymphatic Metastasis , Male , Middle Aged
16.
Biol Pharm Bull ; 28(10): 1864-8, 2005 Oct.
Article in English | MEDLINE | ID: mdl-16204936

ABSTRACT

The present study was investigated the effect of Houttuynia cordata THUNB water extract (HCWE) on mast cell-mediated anaphylactic reactions. The mast cell-mediated anaphylactic reaction is involved in many allergic diseases such as asthma and allergic rhinitis. HCWE has been used as a traditional medicine in Korea and is known to have an antioxidant and anti-cancer activities. However, its specific effect of mast cell-mediated anaphylactic reactions is still unknown. We examined whether HCWE could inhibit compound 48/80-induced systemic anaphylaxis, IgE-mediated passive cutaneous anaphylaxis (PCA), and mast cell activation. The oral administration of HCWE inhibited compound 48/80-induced systemic anaphylaxis in mice. HCWE also inhibited the local allergic reaction, PCA, activated by anti-dinitrophenyl (DNP) IgE antibody in rats. HCWE reduced the compound 48/80-induced mast cell degranulation and colchicine-induced deformation of rat peritoneal mast cells (RPMC). Moreover, HCWE dose-dependently inhibited histamine release and calcium uptake of RPMC induced by compound 48/80 or anti-DNP IgE. HCWE increased the level of intracellular cAMP and inhibited significantly the compound 48/80-induced cAMP reduction in RPMC. These results suggest that HCWE may be beneficial in the treatment of mast cell-mediated anaphylactic responses.


Subject(s)
Anaphylaxis/prevention & control , Drugs, Chinese Herbal/pharmacology , Mast Cells/drug effects , Anaphylaxis/chemically induced , Anaphylaxis/metabolism , Animals , Calcium/metabolism , Cyclic AMP/metabolism , Histamine Release , Houttuynia , Male , Mast Cells/metabolism , Mice , Mice, Inbred ICR , p-Methoxy-N-methylphenethylamine/pharmacology
17.
Exp Mol Med ; 37(4): 290-6, 2005 Aug 31.
Article in English | MEDLINE | ID: mdl-16155406

ABSTRACT

Epigallocatechin gallate (EGCG) is a principle phenolic antioxidant found in a variety of plants, including green and black tea. The anti-allergic effect of EGCG is unknown. The purpose of this study is to investigate the effects of EGCG on compound 48/80-induced mast cell activation and passive cutaneous anaphylaxis. For this, the influences of EGCG on the compound 48/80-induced cutaneous reaction were measured in vivo and the effects of EGCG on the compound 48/80-induced mast cell activations were examined in vitro. Results are below: as 1) EGCG significantly inhibited compound 48/80-induced passive cutaneous anaphylaxis, 2) the compound 48/80-induced degranulation, calcium influx and histamine release of rat peritoneal mast cells (RPMCs) were significantly inhibited by the pretreatment with EGCG, and 3) the compound 48/80-mediated inhibition of cAMP level in RPMCs was significantly increased by the pretreatment with EGCG. These results suggested that EGCG, the most abundant polyphenol in green tea, inhibits the compound 48/80-induced mast cell activation and the increase of vascular permeability, and potentially serve as effective therapeutic tools for allergic diseases.


Subject(s)
Antioxidants/pharmacology , Catechin/analogs & derivatives , Histamine Release/drug effects , Mast Cells/drug effects , Passive Cutaneous Anaphylaxis/drug effects , p-Methoxy-N-methylphenethylamine/antagonists & inhibitors , Animals , Catechin/pharmacology , Cyclic AMP/metabolism , Mast Cells/metabolism , Rats
18.
Zhonghua Nan Ke Xue ; 11(2): 101-3, 2005 Feb.
Article in Chinese | MEDLINE | ID: mdl-15755026

ABSTRACT

OBJECTIVE: To compare two fluorochrome staining methods for the assessment of sperm quality. METHODS: Washed sperm cells were incubated in 0, 0.15, or 15 micromol/L camptothecin (CAM), or 0.37 or 3.7 mmol/L genistein (GEN) at 37 degrees C for 4 hours. The sperm cells were analyzed for cycle-independent apoptosis and necrosis by single-stain compared with dual-stain fluorescence microscopy to contrast the relative effectiveness of these two approaches. RESULTS: The single-stain procedure could not detect the sperm viability differences. In contrast, the dual-stain procedure identified a dosage-dependent decrease in the viability and increased necrozoospermia after topoisomerase inhibitor CAM and GEN treatments. Apoptosis was 2-fold higher with topoisomerase inhibitor treatment. CONCLUSION: The two topoisomerase inhibitors were associated with increased apoptosis and dosage-dependent necrosis. The data suggested that the dual-stain combination Hoechst 33342/PI was more sensitive than the single Hoechst 33342 stain analysis and permitted quantitative analysis of the apoptosis and necrosis in sperm.


Subject(s)
Apoptosis , Spermatozoa/physiology , Staining and Labeling/methods , Apoptosis/drug effects , Benzimidazoles , Camptothecin/pharmacology , Cell Survival/drug effects , DNA/metabolism , Dose-Response Relationship, Drug , Enzyme Inhibitors/pharmacology , Fluorescent Dyes , Genistein/pharmacology , Humans , Male , Propidium , Sensitivity and Specificity , Spermatozoa/drug effects
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