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Am J Reprod Immunol ; 50(1): 83-9, 2003 Jul.
Article in English | MEDLINE | ID: mdl-14506932

ABSTRACT

PROBLEM: The placenta is one of the few non-hematopoietic tissues to express granulocyte colony stimulation factor (G-CSF). Placental G-CSF production is considered to be one of the major causes of granulocytosis during pregnancy although its physiological role in pregnancy has not yet been examined. METHOD OF STUDY: The effects of G-CSF on interleukin (IL)-2 and/or IL-12 induced interferon (IFN)-gamma production of magnetic cell sorting (MACS) sorted decidual lymphocytes was examined by enzyme-linked immunosorbent spot-forming cell assay (ELISPOT). The effect of G-CSF on cytotoxicity of decidual lymphocytes against the choriocarcinoma cell line JEG-3 was examined by lactate dehydrogenase (LDH) release assay. RESULTS: As previously reported by us, IL-2 and/or IL-12 activated decidual mononuclear cells were capable of killing choriocarcinoma cells. We observed that G-CSF abolished IFN-gamma production and cytotoxicity of decidual mononuclear cells and MACS sorted CD56+ cells. CONCLUSIONS: In addition to its well-known trophic effects on hematopoiesis, our results suggest about new roles of G-CSF in reproductive immunology.


Subject(s)
Cytotoxicity, Immunologic/drug effects , Granulocyte Colony-Stimulating Factor/pharmacology , Interferon-gamma/biosynthesis , Interleukin-12/pharmacology , Interleukin-2/pharmacology , Leukocytes, Mononuclear/immunology , Adult , CD3 Complex/analysis , CD56 Antigen/analysis , Cell Line, Tumor/cytology , Cell Line, Tumor/immunology , Decidua/immunology , Dose-Response Relationship, Drug , Drug Interactions , Female , Humans , Interferon-gamma/immunology , Killer Cells, Natural/cytology , Killer Cells, Natural/immunology , Leukocytes, Mononuclear/cytology , Leukocytes, Mononuclear/metabolism , Pregnancy , RNA, Messenger/genetics , RNA, Messenger/metabolism , Receptors, Granulocyte Colony-Stimulating Factor/genetics , Reverse Transcriptase Polymerase Chain Reaction
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