ABSTRACT
The interaction of tricyclazole (TCZ) with beta-cyclodextrin (beta-CD) and human serum albumin (HSA) were studied by fluorescence spectrum, UV-visible spectrum and second-order scattering technology. It was shown that TCZ has quite a strong ability to quench the fluorescence launching from HSA by reacting with it and forming a certain kind of new compound. The quenching and the energy transfer mechanisms were discussed, respectively. The binding constants and thermodynamic parameters at four different temperatures, the binding locality, and the binding power were obtained. The conformation of HSA was discussed by synchronous and three-dimensional fluorescence techniques. The inclusion reaction between beta-CD and TCZ was explored by scattering method, the inclusion constants and the thermodynamic parameters at 297 K and 311 K were figured out, respectively. The mechanism of inclusion reaction was speculated and linkage among the toxicity of TCZ, the exterior environment and its concentration was attempted to explain on molecule level.
Subject(s)
Serum Albumin/metabolism , Spectrometry, Fluorescence/methods , Thiazoles/chemistry , beta-Cyclodextrins/chemistry , Drug Interactions , Energy Transfer , Fluorescence , Humans , Hydrophobic and Hydrophilic Interactions , Molecular Structure , Protein Binding , Spectrophotometry, Ultraviolet , Thermodynamics , Thiazoles/metabolism , beta-Cyclodextrins/metabolismABSTRACT
BACKGROUND: To search for the serological findings and early clinical manifestations as evidences for prevention and treatment TORCH infections in pregnant women and newborns as early as possible. METHODS: ELASA was performed to screen specific anti-TORCH (Toxoplasma gondii, Cytomegalovirus, Rubella virus, Herpes simplex virus) Ig-M antibodies. RESULTS: Totally 1,554 in-patients who were treated in Neonatal Intensive Care Unit (NICU) of our hospital from January 2000 to January 2003 were retrospectively studied, 48 of them had TORCH infections. Cytomegalovirus (CMV), rubella and herpes simplex virus infections accounted for 52.1%, 33.3% and 14.6%, respectively. None of them had toxoplasma infection. CONCLUSION: TORCH infections can cause multiorgan lesions, such as hearing impairment, hyperbilirubinemias and liver dysfunction, impairment of neurologic system, myocardial impairment, thrombocytopenia, and congenital heart disease.Rubella vaccine inoculation, serological screening during pregnancy and early period of newborn, intervention and treatment in the early period are most important.
