Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 3 de 3
Filter
Add more filters










Database
Language
Publication year range
1.
Fish Shellfish Immunol ; 70: 121-128, 2017 Nov.
Article in English | MEDLINE | ID: mdl-28866274

ABSTRACT

Aeromonas hydrophila is the causative agent of bacterial septicemia that is frequently observed in grass carp, Ctenopharyngodon idellus. In this study, we evaluated the biological parameters and immune enzymes in the liver of grass carp following A. hydrophila infection and quantified the alterations in liver histology using a semi-quantitative system. For the biological parameters, we found that the liver somatic index (LSI) was more sensitive than Fulton's condition factor (CF) and was significantly decreased at three days post-injection (DPI). At the immune enzyme level, the level of peroxidase (POD) in the liver significantly increased at 1 and 3 DPI. The activity of alkaline phosphatase (ALP) significantly increased at 3 DPI. Similarly, acid phosphatase (ACP) activity significantly increased at 1, 3, and 5 DPI. Histologically, the results indicated that the liver index at 3, 5, and 7 DPI was significantly higher than that of control groups. The regressive alterations as the highly variable reactions patterns and its index at 5 DPI was significantly higher than that of 1, 21 DPI, and the control groups. Based on our results, we suggest that grass carp resist A. hydrophila infection via an innate immune mechanism in the liver. The findings of this study will help elucidate the underlying mechanisms of resistance to A. hydrophila infection.


Subject(s)
Carps , Fish Diseases/immunology , Gram-Negative Bacterial Infections/veterinary , Immunity, Innate , Liver/immunology , Aeromonas hydrophila/physiology , Animals , Fish Diseases/microbiology , Fish Proteins/genetics , Fish Proteins/metabolism , Gram-Negative Bacterial Infections/immunology , Gram-Negative Bacterial Infections/microbiology , Liver/anatomy & histology
2.
Fish Shellfish Immunol ; 66: 93-102, 2017 Jul.
Article in English | MEDLINE | ID: mdl-28479400

ABSTRACT

The mannan-binding lectin-associated serine protease-1 (MASP-1) gene is a crucial component of the lectin pathway in the complement and coagulation cascade. Although MASP-1 has been found in the immune system of teleosts, its immune functions in response to bacterial infection are unclear. In this study, we identified a MASP-1 homolog (gcMASP-1) in the grass carp (Ctenopharyngodon idella). The full-length 3308-bp gcMASP-1 cDNA includes a 2160-bp open reading frame encoding a protein composed of 719 amino acids with epidermal growth factor-like, complement control protein, and trypsin-like domains. gcMASP-1 shares a high similarity with MASP-1 counterparts in other species, and it is most closely related to Cyprinus carpio MASP-1 and Sinocyclocheilus anshuiensis MASP-1. Transcription of gcMASP-1 was widely distributed in different tissues and induced by Aeromonas hydrophila in vivo and in vitro. Expression of gcMASP-1 was also affected by lipopolysaccharide and flagellin stimulation in vitro. In cells over-expressing gcMASP-1, transcript levels of almost all components, except gcMBL and gcC5, were significantly enhanced, and gcIL1ß, gcTNF-α, gcIFN, gcCD59, gcC5aR1, and gcITGß-2 were significantly upregulated after exposure to A. hydrophila; gcMASP-1 interference downregulated the transcript levels after A. hydrophila challenge. In addition, gcMASP-1 activated NF-κB signaling. These findings indicate the vital role of gcMASP-1 in innate immunity in C. idella.


Subject(s)
Aeromonas hydrophila/immunology , Carps , Fish Diseases/enzymology , Fish Proteins/metabolism , Gram-Negative Bacterial Infections/veterinary , Immunity, Innate/genetics , Mannose-Binding Protein-Associated Serine Proteases/metabolism , Aeromonas hydrophila/physiology , Animals , Cloning, Molecular , DNA, Complementary/genetics , DNA, Complementary/metabolism , Fish Diseases/immunology , Fish Proteins/genetics , Gram-Negative Bacterial Infections/enzymology , Gram-Negative Bacterial Infections/immunology , Mannose-Binding Protein-Associated Serine Proteases/genetics , Phylogeny , RNA, Messenger/genetics , RNA, Messenger/metabolism , Random Allocation , Sequence Analysis, DNA/veterinary
3.
Turk Neurosurg ; 22(4): 506-9, 2012.
Article in English | MEDLINE | ID: mdl-22843477

ABSTRACT

Nummular headache (NH) is defined as a focal head pain that is exclusively felt in a small area, which is typically 1-6 cm in diameter. Neurological examinations are normal in all patients, but this report describes a new variant. A patient was identified who presented with focal head pain that was approximately 8 cm in diameter and bitrigeminal hyperalgesia on neurological examination. Treatment with carbamazepine provided significant analgesic relief in terms of both the frequency and intensity of pain. The findings enlarge the clinical diversity of this headache disorder. The pathogenic mechanisms of NH may be similar to trigeminal neuralgia in particular patients.


Subject(s)
Analgesics, Non-Narcotic/therapeutic use , Carbamazepine/therapeutic use , Headache/drug therapy , Hyperalgesia/drug therapy , Trigeminal Neuralgia/drug therapy , Carotid Stenosis/complications , Carotid Stenosis/diagnostic imaging , Headache/complications , Humans , Hyperalgesia/complications , Male , Middle Aged , Stroke, Lacunar/complications , Stroke, Lacunar/diagnostic imaging , Tomography, X-Ray Computed , Trigeminal Neuralgia/complications
SELECTION OF CITATIONS
SEARCH DETAIL
...