ABSTRACT
Cygnus columbianus jankowskii is a subspecies of Tundra swan, which breeds in eastern Russia and northeast China, wintering in the middle and lower reaches of the Yangtze River. In this study, we used PCR-based method to obtain the complete mtDNA of this subspecies. The arrangement pattern of the complete mtDNA is identical with typical bird species, which is the shortest (16,723 bp) in three subspecies. The length of the PCGs is same except ND2, ND3 and ND6, and the initiation/termination codons are all same with other subspecies. The length of 12S rRNA and 16S rRNA is same with C. c. columbianus, but different from C. c. bewickii. The length and structure of all tRNAs are the same with other subspecies except for tRNA(Ile), tRNA(leu(CUN)) and tRNA(ser(AGY)). The control region is located between tRNA(Phe) and tRNA(Glu), but the length is different with others.
Subject(s)
Anseriformes/genetics , Genome, Mitochondrial/genetics , Sequence Analysis, DNA , Animals , Genes, rRNA , Molecular Sequence Annotation , Molecular Sequence Data , Open Reading Frames/genetics , RNA, Transfer/geneticsABSTRACT
The Swan goose Anser cygnoides is a large threatened goose species in the IUCN Red List, with a natural breeding range in East Asia, migratory and wintering mainly in central and eastern China. In this study, we used PCR-based method to determine the complete mitochondrial genome (mtDNA) of this Anatidae species. The complete mtDNA is a 16,740 bp circular molecule containing 37 typical genes. The gene order is identical with that of the standard avian gene order. All protein-coding genes start with a typical ATG codon except ND5, COI and COII. The termination codon is usually the standard TAA. The non-coding region contains some inter-genic spacers and a control region.
Subject(s)
Anseriformes/genetics , Genome, Mitochondrial , Animals , Avian Proteins/genetics , Codon/genetics , Endangered Species , Gene Order , Regulatory Sequences, Nucleic AcidABSTRACT
Yellow-browed warbler Phylloscopus inornatus inornatus (Passeriformes: Sylviidae) is a small insectivorous leaf-gleaning bird which breeds in forests of the east Palaearctic. In this study, we used PCR-based method to determine the complete mitochondrial genome (mtDNA) of this warbler species. The complete mtDNA is a 16,875 bp circular molecule, containing 37 typical genes and an extra pseudo-control region. The gene order differs from the standard gene order in birds, but similar to genus Sylvia and Acrocephalus, which has the remnant CR2 gene order. All protein-coding genes start with a typical ATG codon. The termination codon is usually the standard TAA, but may be TAG (Cyt b and ND6), AGG (COI) or incomplete T-(COIII and ND4). All tRNAs have the typical clover-leaf structure, except the dihydrouridine (DHU) arm of tRNASer (AGN) and tRNALys (CUN), which forms a simple loop. The non-coding region contains some intergenic spacers, a control region and an extra pseudo-control region.