ABSTRACT
The photosynthetic autotrophic production of microalgae is limited by the effective supply of carbon and light energy, and the production efficiency is lower than the theoretical value. Represented by methanol, C1 compounds have been industrially produced by artificial photosynthesis with a solar energy efficiency over 10%, but the complexity of artificial products is weak. Here, based on a construction of chloroplast factory, green microalgae Chlamydomonas reinhardtii CC137c was modified for the bioconversion of formate for biomass production. By screening the optimal combination of chloroplast transport peptides, the cabII-1 cTP1 fusion formate dehydrogenase showed significant enhancement on the conversion of formate with a better performance in the maintenance of light reaction activity. This work provided a new way to obtain bioproducts from solar energy and CO2 with potentially higher-than-nature efficiency by the artificial-natural hybrid photosynthesis.
Subject(s)
Chlamydomonas reinhardtii , Chloroplasts , Formates , Chloroplasts/metabolism , Formates/metabolism , Chlamydomonas reinhardtii/metabolism , Photosynthesis , Formate Dehydrogenases/metabolism , BiomassABSTRACT
Deoxynivalenol (DON) is extensively detected in many kinds of foods and feeds to harm human and animal health. This research aims to investigate the effect of chlorogenic acid (CGA) on alleviating inflammation and apoptosis of swine jejunal epithelial cells (IPEC-J2) triggered by DON. The results demonstrated that cell viability was decreased when DON concentrations increased or incubation time expanded. The pretreatment with CGA (40 µg/mL) for 1 h increased cell viability, decreased lactate dehydrogenase (LDH) release and apoptosis in cells triggered by DON at 0.5 µg/mL for 6 h, compared with the DON alone-treated cells. Moreover, the mRNA abundances of IL-8, IL-6, TNF-α, COX-2, caspase-3, Bax and ASCT2 genes, and protein expressions of COX-2, Bax and ASCT2 were significantly down-regulated; while the mRNA abundances of ZO-1, claudin-1, occludin, PePT1 and GLUT2 genes, and protein expressions of ZO-1, claudin-1 and PePT1 were significantly up-regulated in the CGA + DON group, compared with the DON alone group. This study indicated that CGA pretreatment alleviated cytotoxicity, inflammation and apoptosis in DON-triggered IPEC-J2 cells, and protected intestinal cell integrity from DON damages.
Subject(s)
Chlorogenic Acid/pharmacology , Protective Agents/pharmacology , Trichothecenes/toxicity , Animals , Apoptosis/drug effects , Caspase 3 , Cell Count , Cell Line , Cell Survival/drug effects , Chlorogenic Acid/metabolism , Epithelial Cells/drug effects , Inflammation/metabolism , Intestines/drug effects , Occludin/genetics , SwineABSTRACT
The data presented in this manuscript showed the effects of the sodium sources on the morphology, crystallite size and carbon content of NaTi2(PO4)3/C composite obtained after heat treatment under N2 atmosphere of the as-spraydried powders. The morphology, crystalline size and carbon content of three products with different sodium sources were investigated. The data are related to "Synthesis of NaTi2(PO4)3@C microspheres by an in situ process and their electrochemical properties" (Mao et al.,2020).
