ABSTRACT
OBJECTIVE: To reveal the anti-tumor effect of micro ribonucleic acid (miR)-127-3p on epithelial ovarian cancer (EOC). PATIENTS AND METHODS: The expression of miR-127-3p in 7 kinds of EOC cell lines and 10 cases of clinical samples of EOC patients was detected via quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR). OVCAR-3 and Caov-3 cell lines were transfected with lentiviruses to overexpress endogenous miR-127-3p. Then, the anti-tumor effect of miR-127-3p on EOC cells was explored through the in vitro cell proliferation assay, bufalin sensitivity assay, wound healing assay, and invasion assay. In addition, whether the mitogen-activated protein kinase 4 (MAPK4) gene is a downstream target of miR-127-3p in EOC was verified via Dual-Luciferase reporter assay and qRT-PCR. The involvement of MAPK4 in regulating phenotypes of OVCAR-3 and Caov-3 cells was finally explored. RESULTS: MiR-127-3p was downregulated in both EOC cell lines and EOC tissues (p<0.05). After lentivirus-mediated overexpression of miR-127-3p, in vitro proliferation and invasion of EOC cells were inhibited, and the sensitivity to bufalin was enhanced (p<0.05). MiR-127-3p directly regulated MAPK4 gene in EOC. Moreover, the upregulation of MAPK4 inhibited the anti-tumor effect of miR-127-3p on EOC, manifested as the remarkably enhanced cell proliferation and migration (p<0.05), and the weakened sensitivity to bufalin (p<0.01). CONCLUSIONS: MiR-127-3p exerts an inhibitory effect on EOC cells via regulating MAPK4 level.
Subject(s)
Carcinoma, Ovarian Epithelial/metabolism , Down-Regulation , MicroRNAs/metabolism , Ovarian Neoplasms/metabolism , RNA Helicases/metabolism , Carcinoma, Ovarian Epithelial/pathology , Cell Line , Cell Movement , Cell Proliferation , Female , Humans , MicroRNAs/genetics , Ovarian Neoplasms/pathology , RNA Helicases/geneticsABSTRACT
OBJECTIVE: The aim of this study was to elucidate the role of long non-coding RNA (lncRNA) CDKN2BAS in aggravating the progression of ovarian cancer via binding growth arrest-specific 6 (GAS6). PATIENTS AND METHODS: The relative levels of CDKN2BAS and GAS6 in ovarian cancer and normal ovarian tissues were detected. In addition, their levels in ovarian cancer cases with different FIGO stages and pathological grades were detected. Pearson correlation test was applied for assessing the correlation between CDKN2BAS and GAS6 levels in ovarian cancer tissues. The roles of CDKN2BAS and GAS6 in mediating proliferative and migratory potentials in HEY and SKOV-3 cells were examined by Cell Counting Kit-8 (CCK-8) and transwell assay, respectively. Subcellular distribution of CDKN2BAS was explored. CDKN2BAS-GAS6 interaction was evaluated by RIP (RNA immunoprecipitation) assay. RESULTS: CDKN2BAS was upregulated in ovarian cancer tissues, especially those with advanced FIGO stage and high pathological grade. It displayed diagnostic potential in ovarian cancer. CDKN2BAS level was positively correlated to that of GAS6 in ovarian cancer tissues. It was mainly expressed in the cytoplasm and could be interacted with GAS6. The overexpression of CDKN2BAS enhanced proliferative and migratory potentials in HEY and SKOV-3 cells. The knockdown of GAS6 partially abolished the regulatory effects of CDKN2BAS on promoting proliferative and migratory potentials in ovarian cancer. CONCLUSIONS: LncRNA CDKN2BAS is upregulated in ovarian cancer. By positively interacting with GAS6, CDKN2BAS triggers the progression of ovarian cancer.
Subject(s)
Intercellular Signaling Peptides and Proteins/metabolism , Ovarian Neoplasms/metabolism , RNA, Long Noncoding/metabolism , Cell Movement , Cell Proliferation , Cells, Cultured , Female , Humans , Intercellular Signaling Peptides and Proteins/genetics , Ovarian Neoplasms/pathology , RNA, Long Noncoding/geneticsABSTRACT
Based on the experience of the late Beijing traditional medicine professor Zhao Bingnan in treating acute infectious febrile disease and its complications, compound herbal drink composed of five kinds of shen. Six hundred cases of febrile disease with damage to Yin and reddened tongue syndrome and hypokalemia. Patients were randomly divided into groups I, II and control. The perspective study of treatment results and experiments were carried out. The results showed that group I and II who received the compound five-Shen herbal drink (CFSHD) showed good therapeutic effects, the rates of significant effectiveness were 65.5% and 73.1% respectively (P greater than 0.05). The total rates of effectiveness were 97.2% and 96.2% respectively. The rate of significant effectiveness of the control group was 11%, total rate of effectiveness was 43%. There was significant statistical difference between the control group and group I or II (P less than 0.001). Authors of present paper are the first to propose the "Reddened Tongue" to be the main diagnostic criterion of syndrome of "Damage both to the Qi and Yin". Laboratory examination of 344 cases of "Reddened Tongue" revealed that 317 cases with hypokalemia (blood potassium less than 3.5 mEq/L) were 92.15% of the entire group. The authors point out that most of the patients with "damage to both the Qi and Yin" besides hypokalemia can be also low in calcium, magnesium and trace elements. After treatment with CFSHD restoration of normal blood potassium was parallel with the improvement of the reddened tongue.(ABSTRACT TRUNCATED AT 250 WORDS)
