Protocol for detecting micro(nano)plastics released from intravenous infusion products.

Approaches for detecting micro(nano)plastics (MNPs) released from intravenous infusion products (IVIPs) are vital for evaluating the safety of both IVIPs and their derived MNPs on human health, yet current understanding is limited. Here, we present a protocol for detecting polyvinyl chloride (PVC) MNPs by combining Raman spectroscopy, scanning electron microscopy equipped with energy-dispersive X-ray spectroscopy (SEM-EDS), and pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS). We describe steps for collecting, pretreating, and measuring PVC MNPs released from IVIPs. For complete details on the use and execution of this protocol, please refer to Li et al.1.

In situ characterization of silver nanoparticles sulfidation processes in aquatic solution by hollow fiber flow-field flow fractionation coupled with ICP-QQQ.

The sulfidation is considered as one of the most important environmental transformation processes of silver nanoparticles (AgNPs), which affects their transport, uptake and toxicity. Herein, based on the hollow fiber flow-field flow fractionation coupled with triple quadrupole inductively coupled plasma mass spectrometry (HF5-ICP-QQQ), we developed an efficient approach to accurately characterize the sulfidation process of AgNPs in aquatic solutions. HF5 could efficiently remove interferential ions and separate nanoparticles with different sizes online, and ICP-QQQ could accurately detect S element through monitoring 32S16O+ in mass shift mode. By the proposed method, two kinds of AgNPs, citrate-coated AgNPs and PVP-coated AgNPs, were selected as models to trace their transfer behaviors during the sulfidation. The results showed once AgNPs were exposed to Na2S solution, the overlapping fractograms of 32S16O+ and 107Ag+ were rapidly detected by HF5-ICP-QQQ to indicate the co-presence of Ag and S, and thus confirming the production of Ag2S and AgNPs underwent a rapid sulfidation process. There were substantial differences in the influence of the two coated agents on the stability of the particles under the conditions examined. In the presence of sulfide, PVP-coated AgNPs could maintain initial size distribution with higher stability, while the size distribution of citrate-coated AgNPs changed considerably. The developed HF5-ICP-QQQ method provides a reliable tool to identify and characterize the transformation process of AgNPs in aquatic solution, which contributed to a deeper understanding of the environmental fate and behavior of AgNPs with different coating.

Nanoparticulate pollutants in the environment: Analytical methods, formation, and transformation.

The wide application of nanomaterials and plastic products generates a substantial number of nanoparticulate pollutants in the environment. Nanoparticulate pollutants are quite different from their bulk counterparts because of their unique physicochemical properties, which may pose a threat to environmental organisms and human beings. To accurately predict the environmental risks of nanoparticulate pollutants, great efforts have been devoted to developing reliable methods to define their occurrence and track their fate and transformation in the environment. Herein, we summarized representative studies on the preconcentration, separation, formation, and transformation of nanoparticulate pollutants in environmental samples. Finally, some perspectives on future research directions are proposed.

Direct entry of micro(nano)plastics into human blood circulatory system by intravenous infusion.

Understanding the pathways of human exposure to micro(nano)plastics (MNPs) is crucial for assessing their health impacts. Intravenous infusion can induce MNPs direct entry into the human blood, posing serious risks on human health, but remains unclear. Herein, we developed comprehensive analytical methods to detect polyvinyl chloride (PVC) MNPs down to 20 nm, and found about 0.52 µg equal to 105-1011 particles of PVC-MNPs released from intravenous infusion products (IVIPs) during each intravenous infusion of 250 mL injection. The released amounts of MNPs from IVIPs were dependent on the plastic materials, and the injection volume and composition. These findings indicated that the released MNPs should be directly introduced into the human blood circulatory system, causing serious impacts on human health. Our study reveals a previously ignored but important pathway of human exposure to MNPs, and calls for further research on the potential risks of these MNPs on human health.

Quantitation of Atmospheric Suspended Polystyrene Nanoplastics by Active Sampling Prior to Pyrolysis-Gas Chromatography-Mass Spectrometry.

Plastic has been demonstrated to release nanoplastics (NPs) into the atmosphere under sunlight irradiation, posing a continuous health risk to the respiratory system. However, due to lack of reliable quantification methods, the occurrence and distribution of NPs in the atmosphere remain unclear. Polystyrene (PS) micro- and nanoplastics (MNPs) represent a crucial component of atmospheric MNPs. In this study, we proposed a simple and robust method for determining the concentration of atmospheric PS NPs using pyrolysis-gas chromatography-mass spectrometry (Py-GC/MS). Following active sampling, the filter membrane is directly ground and introduced into the Py-GC/MS system to quantify PS NPs. The proposed method demonstrates excellent reproducibility and high sensitivity, with a detection limit as low as down to 15 pg/m3 for PS NPs. By using this method, the occurrence of PS NPs in both indoor and outdoor atmospheres has been confirmed. Furthermore, the results showed that the abundance of outdoor PS NPs was significantly higher than that of indoor samples, and there was no significant difference in NP vertical distribution within a height of 28.6 m. This method can be applied for the routine monitoring of atmospheric PS NPs and for evaluating their risk to human health.

Extraction of Common Small Microplastics and Nanoplastics Embedded in Environmental Solid Matrices by Tetramethylammonium Hydroxide Digestion and Dichloromethane Dissolution for Py-GC-MS Determination.

Determination of microplastics and nanoplastics (MNPs), especially small MPs and NPs (<150 µm), in solid environmental matrices is a challenging task due to the formation of stable aggregates between MNPs and natural colloids. Herein, a novel method for extracting small MPs and NPs embedded in soils/sediments/sludges has been developed by combining tetramethylammonium hydroxide (TMAH) digestion with dichloromethane (DCM) dissolution. The solid samples were digested with TMAH, and the collected precipitate was washed with anhydrous ethanol to eliminate the natural organic matter. Then, the MNPs in precipitate were extracted by dissolving in DCM under ultrasonic conditions. Under the optimized digestion and extraction conditions, the factors including sizes and concentrations of MNPs showed insignificant effects on the extraction process. The feasibility of this sample preparation method was verified by the satisfactory spiked recoveries (79.6-91.4%) of polystyrene, polyethylene, polypropylene, poly(methyl methacrylate), polyvinyl chloride, and polyethylene terephthalate MNPs in soil/sediment/sludge samples. The proposed sample preparation method was coupled with pyrolysis gas chromatography-mass spectrometry to determine trace small MPs and NPs with a relatively low detection limit of 2.3-29.2 µg/g. Notably, commonly used MNPs were successfully detected at levels of 4.6-51.4 µg/g in 6 soil/sediment/sludge samples. This proposed method is promising for evaluating small solid-embedded MNP pollution.

Spatial distribution of polystyrene nanoplastics and small microplastics in the Bohai Sea, China.

Micro- and nano-plastic (MNP) pollution has attracted public concerns. Currently, most environmental researches focus on large microplastics (MPs), while small MNPs that have great impacts on marine ecosystems are rarely reported. Understanding the pollution levels and distribution patterns of small MNPs could help assess their potential impacts on the ecosystem. Polystyrene (PS) MNPs were often used as models to assess their toxicity, hence, we collected 21 sites in a Chinese sea area (the Bohai Sea) to analyze their pollution level and horizontal distribution in surface water samples, and vertical distributions in five sites with the water depth >25 m. Samples were filtered by glass membranes (1 µm) to trap MPs, which were frozen, ground, dried, and detected by pyrolysis-gas chromatography-mass spectrometry (pyGC-MS); while the nanoplastics (NPs) in the filtrate were captured with alkylated ferroferric oxide (Fe3O4) to form aggregates, which were separated by glass membrane (300 nm) filtration for pyGC-MS determination. Small PS MPs (1-100 µm) and NPs (<1 µm) were detected in 18 samples with the mass concentrations ranging from <0.015 to 0.41 µg/L, indicating that PS MNPs are widely present in Bohai Sea. Our study contributes to understanding the pollution levels and distribution patterns of MNPs (<100 µm) in the marine system and provides valuable data for their further risk assessment.

Determination of the Particle Number Concentration, Size Distribution, and Species of Dominant Silver-Containing Nanoparticles in Soils by Single-Particle ICP-MS.

The potential risk of various silver-containing nanoparticles (AgCNPs) in soils is related to the concentration, size, and speciation, but their determination remains a great challenge. Herein, we developed an effective method for determining the particle number, size, and species of dominant AgCNPs in soils, including nanoparticles of silver (Ag NPs), silver chloride (AgCl NPs), and silver sulfide (Ag2S NPs). By ultrasonication wand-assisted tetrasodium pyrophosphate extraction, these AgCNPs were extracted efficiently from soils. Then, multistep selective dissolution of Ag NPs, AgCl NPs, and whole Ag NPs/AgCl NPs/Ag2S NPs was achieved by 1% (v/v) H2O2, 5% (v/v) NH3·H2O, and 10 mM thiourea in 2% (v/v) acetic acid, respectively. Finally, the particle number concentration and size distribution of AgCNPs in the extracts and the remaining AgCNP particle number concentration after each dissolution were determined by single-particle inductively coupled plasma mass spectroscopy for speciation of the dominant AgCNPs. AgCNPs were detected in all five soil samples with the concentrations of 0.23-8.00 × 107 particles/g and sizes of 16-110 nm. Ag2S NPs were the main form of AgCNPs in the examined soils with the percentage range of 53.98-69.19%, followed by AgCl NPs (11.42-23.31%) and Ag NPs (7.78-16.19%). Our method offers a new approach for evaluating the occurrence and potential risk of AgCNPs in environmental soils.

Removal of iodide anions in water by silver nanoparticles supported on polystyrene anion exchanger.

The removal of iodide (I-) from source waters is an effective strategy to minimize the formation of iodinated disinfection by-products (DBPs), which are more toxic than their brominated and chlorinated analogues. In this work, a nanocomposite Ag-D201 was synthesized by multiple in situ reduction of Ag-complex in D201 polymer matrix, to achieve highly efficient removal of iodide from water. Scanning electron microscope /energy dispersive spectrometer characterization showed that uniform cubic silver nanoparticles (AgNPs) evenly dispersed in the D201 pores. The equilibrium isotherms data for iodide adsorption onto Ag-D201 was well fitted with Langmuir isotherm with the adsorption capacity of 533 mg/g at neutral pH. The adsorption capacity of Ag-D201 increased with the decrease of pH in acidic aqueous solution, and reached the maximum value of 802 mg/g at pH 2. This was attributed to the oxidization of I-, by dissolved oxygen under the catalysis of AgNPs, to I2 which was finally adsorbed as AgI3. However, the aqueous solutions at pH 7 - 11 could hardly affect the iodide adsorption. The adsorption of I- was barely affected by real water matrixes such as competitive anions (SO42-, NO3-, HCO3-, Cl-) and natural organic matter, of which interference of NOM was offset by the presence of Ca2+. The proposed synergistic mechanism for the excellent performance of iodide adsorption by the absorbent was ascribed to the Donnan membrane effect caused by the D201 resin, the chemisorption of I- by AgNPs, and the catalytic effect of AgNPs.

Total organic carbon content as an index to estimate the sorption capacity of micro- and nano-plastics for hydrophobic organic contaminants.

The worldwide existing micro- and nano-plastics (MNPs) showed high sorption capacity for hydrophobic organic contaminants (HOCs), and thus leading to change of the environmental behaviors and fates of HOCs. However, there is a lack of general index for evaluating the sorption capacity of MNPs for HOCs. Herein, we investigated the sorption of chlorobenzene, naphthalene and phenanthrene to 10 MNPs of different polymer types with and without UV-aging, respectively. It was found that the sorption was well fitted by Freundlich isotherm model with coefficients R2 in the range of 0.892-1.00, and aging of most MNPs resulted in decreased sorption capacity for naphthalene and phenanthrene but slightly increased sorption capacity for chlorobenzene. More importantly, for the 8 MNPs commonly present in the environment and with measured total organic carbon (TOC) covering the range of 23.0-91.9%, the logarithm sorption constant (logKd) values of the studied HOCs positively correlated with TOC contents of MNPs, with a good determination coefficient (R2) of 0.923 for naphthalene, 0.694 for chlorobenzene, and 0.565 for phenanthrene. Our study demonstrated that the TOC content of MNPs is a good index for estimating the contribution of total MNPs to the sorption of nonpolar HOCs in the environmental media.

Swelling-Induced Fragmentation and Polymer Leakage of Nanoplastics in Seawater.

Nanoplastics (NPs) have been successively detected in different environmental matrixes and have aroused great concern worldwide. However, the fate of NPs in real environments such as seawater remains unclear, impeding their environmental risk assessment. Herein, multiple techniques were employed to monitor the particle number concentration, size, and morphology evolution of polystyrene NPs in seawater under simulated sunlight over a time course of 29 days. Aggregation was found to be a continuous process that occurred constantly and was markedly promoted by light irradiation. Moreover, the occurrence of NP swelling, fragmentation, and polymer leaching was evidenced by both transmission electron microscopy and scanning electron microscopy techniques. The statistical results of different transformation types suggested that swelling induces fragmentation and polymer leakage and that light irradiation plays a positive but not decisive role in this transformation. The observation of fragmentation and polymer leakage of poly(methyl methacrylate) and poly(vinyl chloride) NPs suggests that these transformation processes are general for NPs of different polymer types. Facilitated by the increase of surface functional groups, the ions in seawater could penetrate into NPs and then stretch the polymer structure, leading to the swelling phenomenon and other transformations.

Speciation Analysis of Selenium Nanoparticles and Inorganic Selenium Species by Dual-Cloud Point Extraction and ICP-MS Determination.

Application of selenium nanoparticle (SeNP)-based fertilizers results in the release of SeNPs to aquatic systems, where SeNPs may transform into inorganic selenite (Se(IV)) and selenate (Se(VI)) with higher toxicity. However, methods for the speciation analysis of different Se species are lacking, hindering the accurate assessment of the risks of SeNPs. Herein, for the first time, a Triton X-45 (TX-45)-based dual-cloud point extraction (CPE) method was established for the selective determination of SeNPs, Se(IV), and Se(VI) in water. TX-45 can adsorb on the surface of SeNPs and facilitate the extraction of SeNPs into the lower TX-45-rich phase in the first CPE, while Se(VI) and Se(IV) retain in the upper aqueous phase. In the second CPE, Se(IV) can selectively associate with diethyldithiocarbamate and be concentrated in the TX-45-rich phase, whereas Se(VI) remains in the upper phase. Different Se species can be isolated and then quantified by ICP-MS. The presence of coexisting ions and dissolved organic matter (0-30 mg C/L) did not interfere with extraction and separation. The feasibility of the presented method was confirmed by the analysis of natural water samples, with a detection limit of 0.03 µg/L and recoveries in the ranges of 61.1-104, 65.5-113, and 80.3-131% for SeNPs, Se(IV), and Se(VI), respectively. This study aims to provide an effective method to track the fate and transformation of SeNPs in aquatic systems and further contribute to estimating the potential risks of SeNPs to environmental organisms and human bodies.

Influence of particle characteristics, heating temperature and time on the pyrolysis product distributions of polystyrene micro- and nano-plastics.

Pyrolysis-gas chromatography-mass spectrometry (Py-GC/MS) has been widely used for the detection of micro- and nanoplastics (MNPs) in the environment. However, there is a lack of thorough investigation on the effects of pyrolysis temperature and time, as well as the particle source, size and mass of MNPs on the pyrolysis efficiency and pyrolysis product distribution of MNPs. Herein, taking the common plastics polystyrene (PS) as a model, we systematically evaluated the influences of the above factors on the pyrolysis of PS MNPs. Results showed that pyrolysis temperature and time significantly affect the pyrolysis efficiency. By measuring the relative response values of the indicator compound styrene trimers to styrene monomer, the optimum condition was determined as the temperature of 510 â„ƒ and pyrolysis time longer than 18 s. Meanwhile, the mass of MNPs also affected the distribution of PS pyrolysis products. The proportions of styrene dimers and trimers increased slightly with PS MNP mass, while the source, particle size of MNPs have little effect on the pyrolysis product distribution. This work proposed a suitable pyrolysis temperature and time for the determination of PS by Py-GC/MS, which would contribute to the accurate analysis of PS MNPs in the environment.

Evaluating the Occurrence of Polystyrene Nanoparticles in Environmental Waters by Agglomeration with Alkylated Ferroferric Oxide Followed by Micropore Membrane Filtration Collection and Py-GC/MS Analysis.

Although nanoplastics (NPs) are recognized as emerging anthropogenic particulate pollutants, the occurrence of NPs in the environment is rarely reported, partly due to the lack of sensitive methods for the concentration and detection of NPs. Herein, we present an efficient method for enriching NPs of different compositions and various sizes. Alkylated ferroferric oxide (Fe3O4) particles were prepared as adsorbents for highly efficient capture of NPs in environmental waters, and the formed large Fe3O4-NP agglomerates were separated by membrane filtration. Detection limits of 0.02-0.03 µg/L were obtained for polystyrene (PS) and poly(methyl methacrylate) (PMMA) NPs by detection with pyrolysis-gas chromatography-mass spectrometry (Py-GC/MS). When analyzing real water samples from different sources, it is remarkable that PS NPs were detected in 11 out of 15 samples with concentrations ranging from <0.07 to 0.73 µg/L, while PMMA were not detected. The wide detection of PS NPs in our study confirms the previous speculation that NPs may be ubiquitous in the environmental waters. The accurate quantification of PS NPs in environmental waters make it possible to monitor the pollution status of NPs in aquatic systems and evaluate their potential risks.

Total Organic Carbon as a Quantitative Index of Micro- and Nano-Plastic Pollution.

The global pollution of micro- and nano-plastics (MNPs) calls for monitoring methods. As diverse mixtures of various sizes, morphologies, and chemical compositions in the environment, MNPs are currently quantified based on mass or number concentrations. Here, we show total organic carbon (TOC) as an index for quantifying the pollution of total MNPs in environmental waters. Two parallel water samples are respectively filtered with a carbon-free glass fiber membrane. Then, one membrane with the collected particulate substances is treated by potassium peroxodisulfate oxidation and Fenton digestion in sequence for quantifying the sum of MNPs and particulate black carbon (PBC) as TOCMNP&PBC using a TOC analyzer, another membrane is treated by sulfonation and Fenton digestion for quantifying PBC as TOCPBC, and the TOC of MNPs is calculated by subtracting TOCPBC from TOCMNP&PBC. The feasibility of our method is demonstrated by determination of various MNPs of representative plastic types and sizes (0.5-100 µm) in tap, river, and sea water samples, with low detection limits (∼7 µg C L-1) and high spiked recoveries (83.7-114%). TOC is a powerful index for routine monitoring of MNP pollution.

Digestive Elimination of Coexisting Microplastics for Determination of Particulate Black Carbon in Environmental Waters.

Determination of particulate black carbon (PBC) in the environment is of great importance but faces a new challenge due to the increasing occurrence of coexisting microplastics (MPs), which are an emerging contaminant with properties very similar to those of PBC and cannot be discriminated in the chemical digestion procedure of the reported PBC analysis method. Herein, a comprehensive method has been developed for accurately determining PBC by digestive elimination of the coexisting MPs and other non-black carbon organic matter. Water samples were filtered with a glass fiber membrane (0.3 µm pore size), and the collected substances with the membrane were subjected to sulfonation with chlorosulfonic acid and Fenton digestion in sequence and then to the total organic carbon analyzer for quantification of PBC. Under the optimized conditions, MPs of various sizes and polymer types were efficiently eliminated (>91.0%), whereas various PBC samples were undigested with recoveries over 91.7% except for the relatively low recovery of 65.6% for the PBC prepared at a low pyrolysis temperature of 400 °C. The feasibility of the proposed method was verified by analysis of real water samples with a spike recovery of 88.6-100.2%. We anticipate that this work will pave an avenue for reliable determination of PBC in the presence of MPs.

Heteroaggregation of different surface-modified polystyrene nanoparticles with model natural colloids.

This study investigated heteroaggregation of three surface-functionalized polystyrene nanoparticles (PSNPs), i.e. negatively charged unfunctionalized nanoparticles (Bare-PS) and carboxylated nanoparticles (COOH-PS), and positively charged amino-functionalized nanoparticles (NH2-PS), with two model natural colloids, positively charged hematite and negatively charged kaolin, respectively. Heteroaggregation was conducted at a constant natural colloid concentration and variable NP/colloid concentration ratios. Electrostatic interaction was the main mechanism driving the formation of heteroaggregates. In binary systems containing hematite and Bare-PS/COOH-PS, a charge neutralization - charge inverse mechanism was observed with the increase of PSNP concentration. At NP/hemetite concentration ratios much smaller or larger than the full charge neutralization point, the primary heteroaggregates were stable, while full charge neutralization induced the formation of large secondary heteroaggregates. Large aggregates were not observed in suspensions containing kaolin and NH2-PS, as highly positively charged NH2-PS reversed surface charges of kaolin at extremely low concentrations. Heteroaggregation between PSNPs and natural colloids with the same charge is unfavorable due to strong electrostatic repulsion. In the presence of electrolytes, homoaggregation and heteroaggregation both occurred, and homoaggregation of hematite played a key role when the concentration of PSNPs was low. The presence of Suwannee River natural organic matter (SRNOM) could modify surface charges of nanoparticles, and thus affect heteroaggregation behaviors of the binary suspension. When SRNOM and electrolytes were both present, whether SRNOM inducing or hindering the stability of the binary system was a combined effect of NP/colloid concentration ratios, SRNOM concentrations, electrolyte types and ionic strength. Mechanisms extensively reported in homoaggregation such as steric hindrance and cation bridging effects between SRNOM and Ca2+ also stand for heteroaggregation. These results highlight the critical role of surface modification on the environmental behaviors of NPs, and will underpin our understanding of the fate and transport of NPs in the aquatic environment.

Counting Nanoplastics in Environmental Waters by Single Particle Inductively Coupled Plasma Mass Spectroscopy after Cloud-Point Extraction and In Situ Labeling of Gold Nanoparticles.

The globally raising concern for nanoplastics (NPs) pollution calls for analytical methods for investigating their occurrence, fates, and effects. Counting NPs with sizes down to 50 nm in real environmental waters remains a great challenge. Herein, we developed a full method from sample pretreatment to quantitative detection for NPs in environmental waters. Various NPs of common plastic types and sizes (50-1200 nm) were successfully labeled by in situ growth of gold nanoparticles and counted by single particle inductively coupled plasma mass spectrometry. Sucrose density gradient centrifugation enables the isolation of gold-labeled NPs from homogeneously nucleated Au nanoparticles, enhancing the particle number detection limit to 4.6 × 108 NPs/L for 269 nm spherical polystyrene NPs. For real environmental water samples, the pretreatment of acid digestion with a mixture of 5 mM HNO3 and 40 mM HF eliminates the coexisting inorganic nanoparticles, while the following dual cloud-point extraction efficiently isolates NPs from various matrices and thus improves the Au-labeling efficiency. The high spiked recoveries (72.9%-92.8%) of NPs in different waters demonstrated the applicability of this method in different scenarios.

Sequential Isolation of Microplastics and Nanoplastics in Environmental Waters by Membrane Filtration, Followed by Cloud-Point Extraction.

Respective detection of microplastics (MPs) and nanoplastics (NPs) is of great importance for their different environmental behaviors and toxicities. Using spherical polystyrene (PS) and poly(methyl methacrylate) (PMMA) plastics as models, the efficiency for sequential isolation of MPs and NPs by membrane filtration and cloud-point extraction was evaluated. After filtering through a glass membrane (1 µm pore size), over 90.7% of MPs were trapped on the membrane, whereas above 93.0% of NPs remained in the filtrate. The collected MPs together with the glass membrane were frozen in liquid nitrogen, ground, and suspended in water (1 mL) and subjected to pyrolysis-gas chromatography-mass spectrometry (Py-GC/MS) determination. The NPs in the filtrate were concentrated by cloud-point extraction, heated at 190 °C to degrade the extractant, and then determined by Py-GC/MS. For MPs and NPs spiked in pure water, the method detection limits are in the range of 0.05-1.9 µg/L. The proposed method is applied to analyze four real water samples, with the detection of 1.6-7.6 µg/L PS MPs and 0.6 µg/L PMMA MPs in three samples, and spiked recoveries of 75.0-102% for MPs and 67.8-87.2% for NPs. Our method offers a novel sample pretreatment approach for the respective determination of MPs and NPs.

Analytical methods and environmental processes of nanoplastics.

The degradation of plastic debris may result in the generation of nanoplastics (NPs). Their high specific surface area for the sorption of organic pollutions and toxic heavy metals and possible transfer between organisms at different nutrient levels make the study of NPs an urgent priority. However, there is very limited understanding on the occurrence, distribution, abundant, and fate of NPs in the environment, partially due to the lack of suitable techniques for the separation and identification of NPs from complex environmental matrices. In this review, we first overviewed the state-of-the-art methods for the extraction, separation, identification and quantification of NPs in the environment. Some of them have been successfully applied for the field determination of NPs, while some are borrowed from the detection of microplastics or engineered nanomaterials. Then the possible fate and transport of NPs in the environment are thoroughly described. Although great efforts have been made during the recent years, large knowledge gaps still exist, such as the relatively high detection limit of existing method failing to detect ultralow masses of NPs in the environment, and spherical polystyrene NP models failing to represent the various compositions of NPs with different irregular shapes, which needs further investigation.