[Principles of "ecological living water" to purify sewage and its application in black and smelly water treatment of Yangzhou].

To explore the fundamental way of black and odorous water treatment, based on the study of the purification function of natural water body and the analysis of root cause and direct cause of black and odorous water body, we elaborated the scientific connotation and ecological function of "ecological living water", and fully revealed the scientific principle and technical advantages of "ecological living water" for sewage treatment. "Ecological living water" was the original ecological water control in situ. Through the rapid water reaeration, the micro-ecosystem of living water was created. Meanwhile, with the support of micro-speed circulating water, the continuous and efficient purification was realized. The practice of black and odorous water treatment in Yangzhou showed that, 3-5 days after the treatment of "ecological living water", the black and odorous water was completely eliminated and transformed into "green water". 15-20 days after the treatment, water quality reached the national surface water class 3 water standard. Therefore, "ecological living water" may be a kind of sustainable water control technology system with investment of less resources and equipment, fast pollution control speed, low operation cost, good water control effect and both symptoms and treatment.

Bone marrow-derived mesenchymal stem cell transplantation ameliorates oxidative stress and restores intestinal mucosal permeability in chemically induced colitis in mice.

BACKGROUND: Ulcerative colitis (UC) can be viewed as an autoimmune disease. Bone marrow-derived mesenchymal stem cells (MSCs) with its regenerative, cellular multi-lineage and immunomodulatory abilities can influence the repair of damaged tissues in UC. This study investigated the effects of MSCs transplantation on the mice intestinal barrier in response to oxidative stress injury. METHODS: Colitis was induced by daily consecutive administration of 5% dextran sulfate sodium (DSS) solution for 7 days. Male murine MSCs were isolated and transplanted into female mice via injection in the tail vein. Serum and colon specimens were collected at 12 h, 24 h, 3 d, 7 d and 14 d after injection. Serum levels of D-lactate (D-LAC), diamine oxidase (DAO), colonic levels of malondialdehyde (MDA) and superoxide dismutase (SOD) were quantified. The SRY protein of the male sex determinant gene expression and E-cadherin were also ascertained intracellularly. RESULTS: Three days after receiving male MSCs transplantation, SRY protein expression was detected. The quantity increased on successive days. Serum levels of D-LAC and DAO, colonic MDA and SOD normalized in a shorter time period compared to controls (p<0.05). Not surprisingly, histological regeneration of tissue and E-cadherin expression in the colon of MSCs transplanted mice also occurred in a shorter time period than controls. CONCLUSIONS: Transplanted MSCs restored mucosal permeability, and minimized oxidative stress related injury.

[Study of the Raman spectrum of CCl4 Fermi resonance].

Fermi resonance is a very common phenomenon in molecule vibration spectra, especially in polyatomic molecule with complex structure. Fermi resonance appears when a fundamental vibration frequency lies closely to an overtone or combination frequencies. One can observe two peaks coming from Fermi resonance and the energy transfer also occurs between the two peaks. Fermi resonance phenomenon appears in both infrared spectrum and Raman spectrum. The Raman spectrum of CCl4 was measured. The frequency separation between the two peaks in a Fermi resonance doublet was observed and the integrated intensities were calculated with the software of Origin Pro 7.5. Fermi resonance interaction was caused by the CCl4 C-Cl f-symmetrical stretching fundamental nu3 coupling. The combination frequency (nu1 + nu4) of C-Cl a1-symmetrical stretching nu1 and band C-Cl2 f-symmetrical bending nu4 was calculated. Based on Bertran's theory, Fermi coupling coefficient W was calculated and the theoretical value nu3(0) of nu3 was estimated. The present article provided good reference for better understanding of the relationship between molecular vibration frequency and molecular structure.

[Study on clonal proliferation of TCR Vbeta subfamily T cells induced by AML-M(2a) cells in vivo and in vitro].

To observe the expression and clonal expansion of TCR Vbeta subfamily T cells induced by AML-M(2a) cells in vivo and in vitro, complementary determining region 3 (CDR3) of TCR beta with variable region genes was amplified by using RT-PCR in both peripheral blood mononuclear cells (PBMNC) and T cells from mixed lymphocyte and tumor culture (MLTC) from four AML-M(2a) patients. The positive products were further analyzed to identify the clonality of T cells by genescan. The results showed that the similarity distribution of TCR Vbeta subfamily T cells was found Vbeta in PBMNC and MLTC. One or two clonality expansions of T cells could be found in predominant TCR Vbeta subfamily T cells induced by A ML-M2a cells from 3 cases in vivo and in vitro. It was concluded that clonal expansion of TCR Vbeta subfamily T cells stimulated selectively by AML-M(2a) cells may be a specific immune response of patient's T cells to AML-M(2a) cells associated antigen. The clonal proliferation of TCR Vbeta subfamily T cells were affected somewhat by environmental difference in vivo and in vitro.

[The distribution feature of TCR Vbeta repertoire in peripheral blood T cells from patients with Ph(+) and Ph(-) CML].

To investigate the T cell distribution characters of TCR Vbeta repertoire in Ph(+) and Ph(-) CML. The 24 subfamilies of the TCR Vbeta genes were amplified in peripheral blood T cells from 13 patients with CML (Ph+ b3a2, 5 cases; Ph+ b2a2, 5 cases; Ph(-), 3 cases) by RT-PCR, to analyze the usage of Vbeta subfamilies in different CML patients. The results showed that the expression pattern of Vbeta repertoire was different in normal individuals and in patients with CML which only have part of Vbeta subfamily T cells. 4 - 16 (mean 10.2) Vbeta subfamily T cells were detected in the Ph+ b3a2 CML, 8 - 11 (mean 8.8) Vbeta subfamily T cells in the Ph(+) b2a2 CML and 5 - 6 (mean 5.7) in Ph(-) CML. Moreover, the expression of Vbeta subfamily T cells was different among these three types CML.Vbeta10 and Vbeta16 were detected in the all cases with Ph(+) b3a2 and Ph(+) b2a2 CML, whereas Vbeta9 and Vbeta22 could be found in the most cases with Ph(+) b3a2 CML or Vbeta24 and Vbeta8 in Ph(+) b2a2 CML. In patients with Ph(-) CML, Vbeta24 were detected in all samples, and Vbeta9, Vbeta10, Vbeta13 and Vbeta22 were found in the most cases. The results suggest that skew distribution of TCR Vbeta subfamily T cells was existed in peripheral blood of Ph(+) and Ph(-) CML patients. The selected usage of TCR Vbeta is different in various types of CML patients. It may relate to difference of CML cells associated antigen and individual special immunity reaction.